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Featured researches published by Leopoldo Della Ciana.


Journal of the American Chemical Society | 2009

Ru(bpy)3 Covalently Doped Silica Nanoparticles as Multicenter Tunable Structures for Electrochemiluminescence Amplification

Simone Zanarini; Enrico Rampazzo; Leopoldo Della Ciana; Massimo Marcaccio; Ettore Marzocchi; Marco Montalti; Francesco Paolucci; Luca Prodi

The electrochemiluminescence (ECL) of doped silica nanoparticles (DSNPs), prepared by a reverse microemulsion method that leads to covalent incorporation of the Ru(bpy)(3)(2+), was investigated in acetonitrile and aqueous buffers. The emission was produced for the first time by cation-anion direct annihilation, and the position of ECL maxima indirectly allowed estimation of the E(1/2,IOx) and E(1/2,IRed) potentials for Ru(bpy)(3) inside DSNPs. The weak ECL emission is most likely generated by an intrananoparticle ruthenium unit annihilation rather than by the electron transfer between a reduced and oxidized DSNP due to the very low diffusivities of the nanoparticles. Thiol-terminated DSNPs were self-assembled on gold substrates, forming compact and stable monolayers which mimic probe-target assays with DSNPs as labels. The ECL intensity obtained by such functionalized substrates in aqueous media, using tripropylamine (TPrA) as coreactant, was surprisingly increased with respect to direct electrochemical oxidation because of the ability of oxidized TPrA to diffuse within the DSNPs structure and reach a higher number of emitting units with respect to direct electron tunneling. The experimental results have been explained by proposing a basic physical-chemical model which supports evaluation of the number of redox-active centers per nanoparticle. In the model the contrasting effects of increased luminescence quantum yield and decreased diffusion coefficient with respect to free (i.e., not bound within the silica structure) emitting molecules were taken into account. This allows, in principle, optimizing the ECL emission intensity as a function of DSNP size, doping material, charge, doping level, supporting electrolyte, electrode material, and solvent. Finally, it is worth noting that this study has provided a more than 1000-fold increase of the ECL signal of a chemically and electrochemically stable DSNP compared to that of a single dye, suggesting that use of this kind of nanostructures as luminescent labels represents a very promising system for ultrasensitive bioanalysis.


Analytical Chemistry | 2009

Development of a new device for ultrasensitive electrochemiluminescence microscopy imaging.

Luisa Stella Dolci; Simone Zanarini; Leopoldo Della Ciana; Francesco Paolucci; Aldo Roda

Electrochemiluminescence (ECL) is widely used in biosensors and immunoassays thanks to the high sensitivity and specificity of the electrochemically triggered luminescence signal. So far, no applications have been reported on the use of ECL as a probe for ultrasensitive low-light microscope imaging. This work reports the development of a new transparent electrochemical cell for ECL imaging suitable for single cell analysis. The system is based on the use of a microscope placed in a dark box equipped with a CCD camera and a potentiostat. Transparent conducting glass coated with fluorine-doped tin oxide (FTO) has been used, and a three electrode configuration has been designed. The electrochemical cell was optimized using 8 microm diameter polystyrene beads coated with a Ru(bpy)(3)(2+) complex in order to simulate living cells. The Ru(bpy)(3)(2+) immobilized on the microbeads can be imaged and quantified at a concentration as low as 1 x 10(-19) mol/microm(2). Microscope imaging showed that the ECL signal was detected only in correspondence to the beads present on the electrode surface, and the probe could be accurately localized with a spatial resolution of 0.4 microm. The new ECL imaging device can be used in conjunction with other chemiluminescence-based imaging methods for ultrasensitive multiplex imaging on cells and tissues.


Journal of Physical Chemistry B | 2008

Electrochemistry and electrochemiluminescence of [Ru(II)-tris(bathophenanthroline-disulfonate)]4- in aprotic conditions and aqueous buffers.

Simone Zanarini; Leopoldo Della Ciana; Massimo Marcaccio; Ettore Marzocchi; Francesco Paolucci; Luca Prodi

In this work, the electrochemical and ECL properties of tris[1,10-phenanthrolinediyl-4,7-di(benzenesulfonate)]Ru(II) ([Ru(BPS)3]4-) have been addressed in both strictly aprotic conditions and aqueous buffers. A combined theoretical and experimental approach is presented to focus thermodynamics and kinetic effects of electro-generated species possessing highly negative charge. The complex, prepared as the sodium salt by using a newly developed procedure, was subsequently converted to the tetrabutylammonium salt by ion exchange, thus making it soluble in organic media and allowing, for the first time, its thorough electrochemical investigation in ultra-dry aprotic media. The electrochemically induced luminescence (ECL) of Na 4[Ru(BPS)3] in phosphate buffer, using the co-reactant method (tripropylamine), was investigated as a function of the electrode material and halide addition, and ECL intensities six times higher than that of [Ru(bpy)3]2+ were found. In addition, the ECL behavior of this promising dye for biomolecule recognition was investigated in aprotic media and, for the first time, the direct radical anion-radical cation annihilation ECL was obtained.


Analytical Biochemistry | 2008

Chemiluminescent detection systems of horseradish peroxidase employing nucleophilic acylation catalysts

Ettore Marzocchi; Stefano Grilli; Leopoldo Della Ciana; Luca Prodi; Mara Mirasoli; Aldo Roda


Proteomics | 2009

New iodo‐acetamido cyanines for labeling cysteine thiol residues. A strategy for evaluating plasma proteins and their oxido‐redox status

Maurizio Bruschi; Stefano Grilli; Giovanni Candiano; Serena Fabbroni; Leopoldo Della Ciana; Andrea Petretto; Laura Santucci; Andrea Urbani; Rosanna Gusmano; Francesco Scolari; Gian Marco Ghiggeri


Archive | 2009

Active Particles for Bio-Analytical Applications and Methods for Preparation Thereof

Sara Bonacchi; Ricarrdo Juris; Marco Montalti; Luca Prodi; Enrico Rampazzo; Nelsi Zaccheroni; Leopoldo Della Ciana; Serena Fabbroni; Stafano Grilli; Ettore Marzocchi


Analytical Biochemistry | 2009

3-(10′-Phenothiazinyl)propane-1-sulfonate is a potent enhancer of soybean peroxidase-induced chemiluminescence.

Marina M. Vdovenko; Leopoldo Della Ciana; Ivan Yu. Sakharov


Journal of Physical Chemistry C | 2008

Synthesis and Electrochemiluminescence of a Ru(bpy)3-Labeled Coupling Adduct Produced on a Self-Assembled Monolayer

Simone Zanarini; Enrico Rampazzo; Danilo Bich; Roberto Canteri; Leopoldo Della Ciana; Massimo Marcaccio; Ettore Marzocchi; Marco Montalti; Cristina Panciatichi; Cecilia Pederzolli; Francesco Paolucci; Luca Prodi, ,† and; L. Vanzetti


Journal of Immunological Methods | 2010

Development of ultra-sensitive soybean peroxidase-based CL-ELISA for the determination of human thyroglobulin.

Marina M. Vdovenko; Alexander V. Zubkov; Galina I. Kuznetsova; Leopoldo Della Ciana; Nina S. Kuzmina; Ivan Yu. Sakharov


Journal of Chromatography B | 2011

Analysis of the oxido-redox status of plasma proteins. Technology advances for clinical applications ☆

Maurizio Bruschi; Giovanni Candiano; Leopoldo Della Ciana; Andrea Petretto; Laura Santucci; Marco Prunotto; Roberta Camilla; Rosanna Coppo; Gian Marco Ghiggeri

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Aldo Roda

University of Bologna

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