Liesbet Cauwenberg
Katholieke Universiteit Leuven
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Publication
Featured researches published by Liesbet Cauwenberg.
The EMBO Journal | 1998
Sonia Colombo; Pingsheng Ma; Liesbet Cauwenberg; Joris Winderickx; Marion Crauwels; Aloys Teunissen; David Nauwelaers; Johannes H. de Winde; Marie-Françoise Gorwa; Didier Colavizza; Johan M. Thevelein
Adenylate cyclase activity in Saccharomyces cerevisiae is dependent on Ras proteins. Both addition of glucose to glucose‐deprived (derepressed) cells and intracellular acidification trigger an increase in the cAMP level in vivo. We show that intracellular acidification, but not glucose, causes an increase in the GTP/GDP ratio on the Ras proteins independent of Cdc25 and Sdc25. Deletion of the GTPase‐activating proteins Ira1 and Ira2, or expression of the RAS2val19 allele, causes an enhanced GTP/GDP basal ratio and abolishes the intracellular acidification‐induced increase. In the ira1Δ ira2Δ strain, intracellular acidification still triggers a cAMP increase. Glucose also did not cause an increase in the GTP/GDP ratio in a strain with reduced feedback inhibition of cAMP synthesis. Further investigation indicated that feedback inhibition by cAPK on cAMP synthesis acts independently of changes in the GTP/GDP ratio on Ras. Stimulation by glucose was dependent on the Gα‐protein Gpa2, whose deletion confers the typical phenotype associated with a reduced cAMP level: higher heat resistance, a higher level of trehalose and glycogen and elevated expression of STRE‐controlled genes. However, the typical fluctuation in these characteristics during diauxic growth on glucose was still present. Overexpression of Ras2val19 inhibited both the acidification‐ and glucose‐induced cAMP increase even in a protein kinase A‐attenuated strain. Our results suggest that intracellular acidification stimulates cAMP synthesis in vivo at least through activation of the Ras proteins, while glucose acts through the Gpa2 protein. Interaction of Ras2val19 with adenylate cyclase apparently prevents its activation by both agonists.
Enzyme and Microbial Technology | 2000
Johan M. Thevelein; Liesbet Cauwenberg; Sonia Colombo; Johannes H. de Winde; Monica Donation; Françoise Dumortier; L. Kraakman; Katleen Lemaire; Pingsheng Ma; David Nauwelaers; Filip Rolland; Aloys Teunissen; Patrick Van Dijck; Matthias Versele; Stefaan Wera; Joris Winderickx
Fems Yeast Research | 2001
Filip Rolland; Valeria Wanke; Liesbet Cauwenberg; Pingsheng Ma; Eckhard Boles; Marco Vanoni; Johannes H. de Winde; Johan M. Thevelein; Joris Winderickx
Fems Yeast Research | 2001
Filip Rolland; Valeria Wanke; Liesbet Cauwenberg; Pingsheng Ma; Eckhard Boles; Marco Vanoni; J Dewinde; Johan M. Thevelein; Joris Winderickx
Archive | 2002
Johan M. Thevelein; Katleen Lemaire; Matthias Versele; M. Donaton; Filip Rolland; Liesbet Cauwenberg; Inge Holsbeeks; Frank Giots; R Gelade; Ole Lagatie; Joris Winderickx; Patrick Van Dijck; Stefaan Wera
Yeast | 2001
Liesbet Cauwenberg; Joris Winderickx; Johan M. Thevelein
Yeast | 2001
Joris Winderickx; Filip Rolland; Liesbet Cauwenberg; Katleen Lemaire; M. Donaton; Johan M. Thevelein
Archive | 2000
Liesbet Cauwenberg; Joris Winderickx; Johan M. Thevelein
Archive | 2000
Johan M. Thevelein; Katleen Lemaire; Matthias Versele; M. Donaton; Filip Rolland; Liesbet Cauwenberg; Inge Holsbeeks; Joris Winderickx; Patrick Van Dijck; Stefaan Wera
Archive | 2000
Johan M. Thevelein; Katleen Lemaire; Matthias Versele; M. Donaton; Filip Rolland; Liesbet Cauwenberg; Inge Holsbeeks; Joris Winderickx; Patrick Van Dijck; Stefaan Wera