Liliane Suguisawa

Growth and carcass traits associated with GH1/Alu I and POU1F1/Hinf I gene polymorphisms in Zebu and crossbred beef cattle

The objectives of the present study were to estimate the allele and genotype frequencies of the GH1/Alu I and POU1F1/Hinf I polymorphisms in beef cattle belonging to different genetic groups and to determine the effects of these polymorphisms on growth and carcass traits in cattle submitted to feedlot management, an intensive production model. Genotyping was performed on 384 animals, including 79 Nellore, 30 Canchim (5/8 Charolais + 3/8 Zebu), 30 Simmental x Nellore crossbred and 245 Angus x Nellore crossbred cattle. Body weight, weight gain, dressing percentage, Longissimus dorsi area and backfat thickness were fitted using the General Linear Model (GLM) procedure of the SAS program and the least square means of the genotypes were compared using the F test. The results showed significant associations between the LL genotype of the GH1/Alu I polymorphism and higher weight gain and body weight at slaughter (p < 0.05). The POU1F1/Hinf I polymorphism did not have any effect on the growth and carcass traits analyzed.

Growth hormone 1 gene (GH1) polymorphisms as possible markers of the production potential of beef cattle using the Brazilian Canchim breed as a model

The growth hormone 1 gene (GH1) is a candidate gene for body weight and weight gain in cattle since it plays a fundamental role in growth regulation. We investigated the GH1 gene AluI and DdeI restriction enzyme polymorphisms, located 149 bp apart in the cattle genome, as possible markers of the production potential of Canchim crossbreed cattle, a 5/8 Charolais (Bos taurus) and 3/8 Nelore (Bos indicus) breed developed in Brazil, by evaluating the birth weight, weaning weight, yearling weight and plasma insulin-like growth factor-1 (IGF-1) concentration of 7 month to 10 months old Canchim calves (n = 204) of known genealogy and which had been genotyped for the AluI and DdeI markers. Our results showed significant effect (p < 0.05) between the homozygous DdeI+/DdeI+ polymorphism and the estimated breeding value for weaning weight (ESB-WW), while the AluI leucine homozygous (L/L) and leucine/valine (L/V) heterozygous polymorphisms showed no significant effect on the traits studied. The restriction sites of the two enzymes led to the formation of haplotypes which also exerted a significant effect (p < 0.05) on the ESB-WW, with the largest difference being 8.5 kg in favor of the homozygous L plus DdeI+/L plus DdeI+ genotype over the heterozygous L plus DdeI-/V plus DdeI+ genotype.

Correlações simples entre as medidas de ultra-som e a composição da carcaça de bovinos jovens

The objective of this study was to evaluate correlations between ultrasonography measurements and carcass traits of 115 steers (Nellore, ½ Angus-Nellore, ½ Simmental-Nellore, and Canchim) averaging 329 kg of initial body weight and two different finishing frame sizes (small and large). After 120 days in a feedlot, body weight, rib eye area (RA), and fat thickness (FT) were measured by ultrasound. Following slaughter, carcass RA and FT measurements and weights of hindquarter, forequarter, and commercial cuts were all taken; body composition was also determined. Yields of carcass, retail cuts, and hindquarter as well as ratios of ultrasound RA per 100 kg of body weight and ultrasound RA per 100 kg of carcass weight were calculated. Data showed higher muscle deposition in ½ Simental-Nellore and Canchim and greater fat deposition in Nellore while ½ Angus-Nellore steers combined both characteristics. According to the results, RA was a good indicator of the proportion of muscle present in the carcass and selection for increasing carcass RA may decrease FT because of the negative correlation between FT and hindquarter percentage and RA. Frame size did not affect carcass composition possibly due to the small variation between both groups. Because correlations of measured and ultrasound RA and FT with carcass components yielded similar results, it was possible to validate ultrasound measurements to predict carcass composition of young bulls.

Ultra-sonografia para predição da composição da carcaça de bovinos jovens

This study was conducted to evaluate the use of ultrasound measurements to predict carcass composition of 115 steers (Nellore, ½ Angus x Nellore, ½ Simental x Nellore, and Canchim) with different frame sizes (small and large) and average initial body weight of 329 kg. After 120 days in a feedlot, animals were weighed followed by ultrasound measurements of rib eye area (RA) and fat thickness (FT). Animals were slaughtered and measurements of carcass RA and FT, weights of hindquarter, forequarter, and commercial cuts, and determination of body composition were done. In addition, yields of carcass, commercial cuts, and hindquarter were calculated and no significant differences between frame sizes were found for these variables. Ultrasound measurements were not precise predicting carcass muscle content and yields of commercial cuts in this trial. However, determination coefficients from regression equations of carcass composition using ultrasound were similar and sometimes higher (e.g. bone content) than those from regression equations of carcass composition, in which measurements were taken after slaughter. Therefore, ultrasound measurements can be used to predict carcass composition of beef cattle for some variables.

Nutritional plans for boars

The objective of the present study was to evaluate nutritional plans for boars. Four hundred animals of 67 to 135 days of age and initial weight of 27.75±1.61 kg were distributed in a randomized block design with seven nutritional plans for boars (9.0-8.0; 9.0-9.0; 10.0-9.0; 10.0-10.0; 11.0-10.0; 11.0-11.0 and 12.0-11.0 g/kg of digestible lysine from 67 to 107 days and from 108 to 135 days, respectively) with four repetitions and a control plan for barrows (11.0-10.0 g/kg of digestible lysine) with eight repetitions and ten animals each. Uncastrated male swine presented better feed conversion; however they showed a lower marbling degree in relation to barrows, regardless of the nutritional plan. The nutritional plan that corresponds to the sequence of 11.0-10.0 g/kg of digestible lysine from the 67 to the 107 days and from the 108 to the 135 days, respectively, meets the nutritional needs of boars.

Efeitos da transformação de uma variável com distribuição normal em sua inversa sobre os parâmetros de sua distribuição usando técnicas de Monte Carlo

Four simulation studies were conducted to verify the distribution of the inverse of variables with normal distribution, relatively to variances, averages, truncation points and sample sizes. The variables simulated were GMD, with normal distribution and representing average daily gain, and DIAS defined as a multiple of the inverse of GMD and representing days to reach a fixed body weight. The SAS® (1990) system was used, for simulation of the data, and for subsequent analysis of the results in all studies. The standard deviations simulated for GMD significantly affected DIAS sampling averages. The regression analyses showed a reduction on the mean and in the standard deviation of DIAS as a function of the increase in the average of GMD. Including a truncation point at about 10 to 25% of the mean value reduced the mean of GMD and increased the mean of DIAS when the coefficient of variation of GMD was above 25%. Size of the groups did not significantly affect averages of GMD or DIAS. Standard deviation and CV of GMD increased with the increase on group size. Due to the dependence between the average and the standard deviation and the variation observed in the standard deviations of DIAS as a function of group size, the use of DIAS as selection criteria may reduce the accuracy of the genetic evaluation. Therefore, in order to substitute GMD by DIAS, it is necessary the use of a method of analysis robust enough to eliminate the heterogeneity of variance.

Efeito de valores discrepantes em grupos de contemporâneos na predição dos valores genéticos

Nine populations, with five replications each, were simulated for average daily gain (GMD1) assuming normal distribution, average 100, and different group sizes as well as standard deviations. Each replication was divided into three different sets of contemporary (CG) and progeny groups. The first set (I) was formed by 1,000 contemporary groups, 100 observations each and 10 progenies/sire. The second set (II) included 2,500 CG, 40 observations and 4 progenies/sire. The third set (III) consisted of 5,000 CG, 20 observations and two progenies/sire. The number of records per group and sire and the number of sires per group varied within the same set. In each population, GMD1 was transformed by DIAS1=100/GMD1. The contribution of CG to sire breeding value predictions were calculated for GMD1 (Cx) and DIAS1 (Cy). The maximum and the average of DIAS1 effects on Cy absolute value were significant, but the R2 were low (maximum of 16%). The minimum value of DIAS1 has not influenced Cy. The maximum and the minimum of GMD1 on Cx were significant, but the R2 were very low (maximum 2%). The GMD1 average has not affected Cx. The effect of the minimum value of DIAS1 was important to determine the CG effect on breeding value of sires with progeny in the set. A very low value of GMD1 for an animal corresponds to a high value of DIAS1 and affects the values of the other animals and the CG average. This effect has an impact on the evaluation of their sires and will be an important source of error in the genetic evaluation of the herd. Thus, the replacement of GMD1 by DIAS1 as selection criteria for growth is not recommended because it will reduce the possibility of genetic gain.