Liming Zhang
Tianjin University of Science and Technology
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Featured researches published by Liming Zhang.
Brazilian Archives of Biology and Technology | 2011
Yugao Wang; Liming Zhang; Xinglin Li; Wenyuan Gao
The starches obtained from two different yam residues, which were treated with alkali(starch-A) or enzyme (starch-E), were studied and compared with yam starch isolated using ordinary method (starch-O) for morphological, crystalline pattern, thermal, and pasting properties. The results revealed that the amylose content of three starches ranged from 19.47 to 22.17%. The granule surfaces of starch-A and starch-E were as smooth as that of starch-O. The crystalline pattern of the three starches was a C-type. The transition temperatures (To, Tp and Tc) varied from 70.11 to 73.64, 79.23 to 81.74, and 84.30 to 86.65 oC, respectively. The starch-E showed the highest Δ Hgel, followed by the starch-A, while it was lowest for the starch-O. According to the viscosity measurement, starch-O had the lowest pasting temperature, highest peak viscosity and breakdown viscosity, which were contrary to those of starch-E.
Journal of Agricultural and Food Chemistry | 2016
Peiyu Qiu; Shuli Man; Jing Li; Jing Liu; Liming Zhang; Peng Yu; Wenyuan Gao
Curcumin is the major active component of turmeric and widely used as a spice and coloring agent in food. However, its safety evaluation has been little investigated. To evaluate the 90-day subchronic toxicity of curcumin in rats, its general observation, clinical biochemistry, pathology, and metabolomics were evaluated. The results showed that curcumin induced liver injury through the generation of the overexpression of reactive oxygen species (ROS) and pro-inflammatory cytokines IL-6 and the decreases of the levels of antioxidant enzyme SOD and detoxified enzyme GST. Meanwhile, for the self-protection of rats, curcumin treatment activated the transcription of Nrf-2 and elevated the expression of HO-1 to reduce tissue damage. Furthermore, curcumin significantly increased key mRNA levels of HK2, PKM2, LDHA, CES, Cpt1, Cpt2, FASN, and ATP5b and decreased levels of GLUT2 and ACC1 to enhance glycolysis and inhibit lipid metabolism and TCA cycle. Therefore, overdose or long-term intake of curcumin could initiate the unbalanced state of bodies through oxidative stress, inflammation, and metabolic disorders, which induces liver injury. Intermittent administration of curcumin is necessary in our daily lives.
Acta Physiologiae Plantarum | 2014
Hui Liu; Juan Wang; Wenyuan Gao; Qin Wang; Liming Zhang; Shuli Man
In this study, adventitious roots of Panax quinquefolium L. have been successfully established. The highest induction rate of roots was obtained in MS medium containing 3xa0mgxa0L−1 IBA after 4xa0weeks of culture. The culture conditions of adventitious root were optimized and evaluated with response surface methodology. The best culture conditions for root growth seemed to be 0.75 salt strength MS medium, 4.70xa0gxa0L−1 inoculum size and 40xa0days of culture. The active component contents of adventitious roots were compared with those of native roots. The total saponins content was found to be 16.28xa0mgxa0g−1 in native root and 4.64xa0mgxa0g−1 in adventitious root. The polysaccharide content of the adventitious root was 1.5 times higher than that in the native P. quinquefolium (30.54 vs. 20.28xa0mgxa0g−1).
Acta Physiologiae Plantarum | 2013
Songbo Guo; Shuli Man; Wenyuan Gao; Hui Liu; Liming Zhang; Peigen Xiao
In this study, callus and cell suspension were induced from seedlings of licorice (G. uralensis). In addition, it was revealed that the appropriate concentration of sucrose could promote the callus growth and increase the content of polysaccharide. The methyl jasmonate (MJ) and phenylalanine (PHE) could enhance the callus growth and content of flavonoids for G. uralensis. For producing more flavonoids and polysaccharide, two-stage cultivation was performed. In the first step, 30xa0gxa0L−1 sucrose was fed into a 5-L balloon-type bubble bioreactor on 8th day of culture to enhance cell production and metabolite production. In a two-stage cultivation process, PHE (2xa0mM) and MJ (5xa0mgxa0L−1) were added into a 5-L balloon-type bubble bioreactor after 10 days of culture. Using a fed-batch cultivation strategy (30xa0gxa0L−1 sucrose was fed into a 5-L balloon-type bubble bioreactor on 8th day), polysaccharide production was enhanced to 1.19xa0gxa0L−1, which was 2.12-fold greater than that in batch cultivation. The flavonoids yield (55.42xa0mgxa0L−1) which was about 22xa0% higher than that in batch cultivation was obtained on 21stxa0day. In a two-stage cultivation process, the polysaccharide content was increased by 1.14- and 2.12-fold compared with fed-batch cultivation and batch cultivation on 15th day. Meanwhile, total flavonoids yield (132.36xa0mgxa0L−1) on 15th day, was increased by 2.26- and 2.67-fold compared with fed-batch cultivation and batch cultivation. In conclusion, two-stage cultivation process combined with the sucrose and elicitor treatment could promote both the callus growth and the secondary metabolites accumulation.
Analytical and Bioanalytical Chemistry | 2013
Shuli Man; Songbo Guo; Wenyuan Gao; Juan Wang; Liming Zhang; Xinglin Li
AbstractGlycyrrhiza uralensis has long been used as a flavoring and sweetening agent in food products. In the last ten years, suspensions of Glycyrhiza cells have been successfully established. However, there is no report of full metabolic profiling research on these cells. To identify their composition we used HPLC–DAD coupled with ESI(+/−)–MSn to compare the constituents of cultured Glycyrhiza (CG) cells with those the native cells (NG). We identified 60 compounds including flavonoids, phenols, and triterpenoids. Among these compounds, 42 occurred both in NG and CG, nine were present in NG only and nine were present in CG alone. The number of the triterpenoid aglycones without glycones in CG was smaller than that in NG. The number of flavanone, isoflavone, isoflavan, and benzenoid compounds was also smaller in CG than that in NG, whereas the number of pterocarpans was much higher. Although differences existed between CG and NG, the extract of CG was similar to that of NG. With the development of cell suspension culture-based biotransformation, cell culture of Glycyrrhiza has the potential to be more profitable than field cultivation in some areas.n
Environmental Toxicology | 2017
Shuli Man; Peiyu Qiu; Jing Li; Liming Zhang; Wenyuan Gao
Rhizoma Paridis saponins (RPS) is a traditional Chinese medicine (TCM) from the plant Paris polyphylla var. yunnanensis (Fr.) Hand.‐Mazz. Despite its potentially clinical utility such as anticancer and anti‐inflammation, it has slight side effects and toxicity as previous report. In this work, 90‐day administration of RPS induced liver injury. 1H‐NMR‐ and GC/MS‐based metabonomic analyses in conjunction with histopathological examinations, blood biochemistry and hepatic phase I and II enzymes assays were performed to evaluate the toxic mechanisms of RPS induced in rats. As a result, oral administration of RPS possessed certain liver toxicity in SD rats. 1H‐NMR and GC/MS data indicated that RPS inhibited the oxidation of fatty acids, glycolysis, and TCA cycle pathway, and disturbed glycine, serine, and threonine metabolism. Low expression of TG, T‐CHO, and LDL‐C and high levels of ALT and AST indicated that chronic exposure to RPS caused hepatocyte damage, synthesis dysfunction, and transportation failure of lipoproteins. In addition, RPS downregulated the mRNA levels of CYP1A2, CYP2E1, and UGTs. In conclusion, we used metabonomics approach to study the toxicity of RPS for the first time. This research demonstrated that metabonomics method was a promising tool to study and diagnose TCM‐induced toxicity.
RSC Advances | 2016
Jing Li; Shuli Man; Peiyu Qiu; Wei Fan; Liming Zhang; Wenyuan Gao
The aim of the study is to evaluate the safety of long-term use of Rhizoma paridis saponins (RPS). After 90 day administration of RPS in rats, it induced liver and lung injury through the over-expression of reactive oxygen species (ROS) and pro-inflammatory cytokines, and by down-regulating the levels of antioxidant and detoxification enzymes. Meanwhile, RPS treatment also activated the self-protective transcription of Nrf2 and elevation of GSH and HO-1 expression to inhibit worsening tissue conditions in the rats. After 30 days recovery, the abnormalities in liver and lungs disappeared, accompanied by the return of phase II enzyme, pro-inflammatory cytokine, and nuclear factor levels to normal. In conclusion, 350 mg kg−1 d−1 of RPS induced toxicity and detoxicity reactions involving NF-κB and Nrf2 signaling. Our work provides useful data for the correct administration of RPS and minimizing the danger of toxic herbal product use.
Research on Chemical Intermediates | 2015
Juan Wang; Wenyuan Gao; Qin Wang; Shuli Man; Liming Zhang
In this study, based on the time profiles of growth and specific oxygen uptake rate in Panax ginseng adventitious root, step-wise aeration treatment was conducted using a 5-L balloon-type bubble bioreactor to enhance root density and metabolite production. The results showed that step-wise aeration of 0.3–0.5–0.3xa0vvm was found to be optimal for the accumulation of biomass, total saponins content, and polysaccharide content. Such optimization of the aeration volume will be beneficial for the large-scale production of biomass and bioactive compounds of ginseng adventitious root.
African Journal of Biotechnology | 2011
Xinglin Li; Dandan Wang; Jie Gao; Tianjian Xiao; Xiaojuan Wang; Liming Zhang; Dong Liu; Limei Li; Fuping Lu; Wenyuan Gao
To find out a simpler method that can directly transfer the aim gene into plant genomes, the purple medic seeds irradiated by 60 Co with 0.375 Gy were transformed by linear DNA containing a β-glucuronidase (GUS) gene (as an aim gene), a betaine aldehyde dehydrogenase (BADH) gene (as a selectable marker) and two pairs of both CaMV35S promoter and Nos terminator. Subsequently, the seeds were planted and grown in perlite media watered with NaCl solution as a kind of selective compound. The results showed that, positive frequency of PCR identification by the GUS gene or the BADH gene was higher than 53.2 and 89.5% in T 0 and T 1 generations, while GUS staining rate was higher than 50%; whereas five T 1 plants assayed by southern hybridization all showed positive reaction. In conclusion, by this method, transgenic plants may be easily obtained with the antibiotic markers for free; moreover, the plant regeneration-system must not be erected by directly transforming the seeds. Key words : DNA transformation, irradiated seeds, purple medic, salt screening.
Thermochimica Acta | 2009
Liming Zhang; Weiguang Xie; Xi Zhao; Ying Liu; Wenyuan Gao