Lina Herlina

The Conservation Efforts of Wood Apple, An Identity Plant of Rembang Regency, Based on Ethnobotany Study

Feronia limonia (L.) Swingle better known as wood apple is an identity plant of Rembang Regency, Central Java Province, Indonesia. However, the population is very limited. In order to identify the conservation efforts, this ethnobotany study was aimed to determine traditional knowledge on the existence, economic value, utility, breeding technique and conservation of wood apple. The survey was conducted on a total of 102 local people from 10 sub-districts which were determined by proportionally stratified random sampling. Data were collected by questionnaires and individual interviews and then were analyzed descriptively. The results showed that all of respondents knew the figure of wood apple, but only 79.50% understood its status as an identity plant. Most of respondents (96%) stated that the fruits have low economic value, 80.39% respondents have utilized ripe fruit as ingredients of beverages and stems as firewood. This utilization was only equal to 29.72% compared to the potential usability based on research results. It was concluded that the traditional knowledge about the existence, economic value, and breeding technique were good and on the contrary, the knowledge about the plant’s usability was bad. The results will form a basic knowledge for in-depth studies to provide a government policy of Rembang Regency in order to develop an efficient strategy of management and conservation of wood apple as plant identity

Optimasi Konsentrasi 2,4-D, Ba, dan Lama Penyinaran untuk Memacu Regenerasi Tunas dari Kalus Kedelai

Alternatif untuk mengatasi kualitas kedelai yang rendah yaitu perbaikan sifat genetik. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi 2,4-Dichlorophenoxyacetic Acid (2,4-D) dan Benzyl Adenine (BA) serta lama penyinaran dan interaksinya terhadap regenerasi tunas dari kalus kedelai, dan untuk menentukan interaksi faktor-faktor yang paling optimal dalam regenerasi tunas dari kalus kedelai. Konsentrasi 2,4-D dan BA masing-masing terdiri dari 4 taraf (0 ppm; 3 ppm; 6 ppm; 9 ppm) dan 2 taraf lama penyinaran (24 jam dan 0 jam). Analisis menggunakan ANAVA tiga arah dan uji lanjut Duncan. Parameter yang diamati adalah waktu muncul tunas, panjang tunas, jumlah tunas, dan persentase kalus membentuk tunas. Hasil menunjukkan bahwa konsentrasi BA dan lama penyinaran mempengaruhi regenerasi tunas dari kalus kedelai, sedangkan konsentrasi 2,4-D tidak berpengaruh signifikan terhadap regenerasi tunas. Konsentrasi BA yang paling optimal adalah 3 ppm dan lama penyinaran yang optimal adalah kondisi 0 jam. Interaksi konsentrasi BA, 2,4-D dan lama penyinaran berpengaruh terhadap regenerasi tunas terutama pada banyaknya jumlah tunas yang dihasilkan. Perlakuan BA 3 ppm + 2,4-D 6 ppm + 24 jam adalah perlakuan yang optimal dalam meregenerasi tunas dengan jumlah kalus yang banyak. Berdasarkan hasil penelitian disarankan untuk meregenerasi kalus menggunakan ZPT BA dan 2,4-D dan Lama penyinaran. Genetic trait improvement is a way to overcome the low quality soybean. The aim of this research is to determine the effect of 2,4-Dichlorophenoxyacetic Acid (2,4-D) and Benzyl Adenine (BA) concentration and long irradiation and their interaction on bud regeneration from callus soybean, and to determine the interaction of the factors that most optimal in bud regeneration from callus soybean. 2,4-D and BA concentration each consisting of 4 levels (0 ppm; 3 ppm; 6 ppm; 9 ppm) and 2 levels long irradiation (24 hours and 0 hour). Analysis using a three -way ANAVA and Duncan test further. Parameters measured were the time appears bud, bud length, number of buds, and the percentage of callus forming buds. The result showed that BA concentration and long irradiation affects the regeneration of shoots from callus soybean, whereas the concentration of 2,4-D had no significant effect on bud regeneration. The most optimal concentration of BA is 3 ppm and optimal long irradiation is the condition 0 hours. BA concentration, 2,4-D concentration and long irradiation interaction effect on the regeneration of buds mainly on the number of buds produced. Treatment BA 3 ppm + 2,4-D 6 ppm + 24 hours is the optimal treatment in regenerating buds the number of callus that many. Based on the research results suggested to regenerate callus using ZPT BA and 2,4-D and long irradiation.