Liu Shuying

The Application of HPLC-ESI-MS or FRAP in Studying the Variation of Ginsenosides and Their Anti-oxidation after the Combination of Ginseng with Zingiber officinale Rosc or Radix Paeoniae Rubra

To study the content variation of ginsenosides after the combination of ginseng with Zingiber officinale Rosc or Radix Paeoniae Rubra,a high performance liquid chromatography-electrospray ionization spectrometric(HPLC-ESI-MS) method was used to analyze and compare the ginsenosides in the decoction.The experimental results show that the contents of ginsenosides decreased along with the increased amount of Zingiber officinale Rosc,while the stripping of ginsenosides was promoted by a small amount of Radix Paeoniae Rubra.In addition,the total antioxidant activity of n-butanol and water extracts from the single and compatible decoctions of ginseng was also determined by ferric reducing antioxidant power(FRAP) method.With ascorbic acid(500 μmol/L) as control,the anti-oxidation of compatible decoction of ginseng with Zingiber officinale Rosc/Radix Paeoniae Rubra was higher than that of the single decoction of ginseng;and the FRAP values of n-butanol extracts from the compatible decoction of ginseng with Zingiber officinale Rosc or Radix Paeoniae Rubra were 1562.29 and 2969.78 μmol/L,respectively,greater than the sum of single decoction of ginseng with Zingiber officinale Rosc(1260.27 μmol/L) /Radix Paeoniae Rubra(2502.07 μmol/L),indicating that the antioxidant activity of n-butanol extract from compatible decoction was better.

Applications of Modern Mass Spectrometry and Its Coupling Technologies in Schisandrae Fruits Chemical Research

Abstract The applications of mass spectrometry (MS) and its coupling technologies (gas chromatography-MS, high-performance liquid chromatography-MS, capillary electrophoresis-MS, etc.) on the chemical constituents of lignans, essential oils, organic acids, and triterpenoids in ( Schisandra chinensis and Schisandra sphenanthera ) fruits are reviewed and their potential applications are summarized. The aim of this study is to provide a basis for rapid screening of chemical constituents from Chinese medicine, identification of medicinal material varieties, and quality control methods.

Gas phase ion-molecule reactions of buckminsterfullerene C 60 with some small organic compounds in mass spectrometer

In chemical ionization mass spectrometry (CIMS) gas phase C-60(+) or C-60 can react with fragment ions from three chloromethane and four multichloroethane molecular ions via ion-molecule reactions. A dozen of gas-phase adduct ions of C-60 are observed, and most of them contain chlorine atoms. The results of the comparison and analysis show that the relative intensities of adductions are not directly proportional to the corresponding fragment ions in the MS of reagents,which implies that some fragment ions containing radicals are more reactive with C-60(+) or C-60. This indicates that the alkene-like C-60(+) or C-60 can act as a radical sponge in addition reactions.