Lizyana Vieira

Nociceptive and Neuronal Evaluation of the Sciatic Nerve of Wistar Rats Subjected to Compression Injury and Treated with Resistive Exercise

Background. To investigate the climb stairs resistance exercise on nociception and axonal regeneration in the sciatic nerve of rats. Methods. 24 Wistar rats were divided: control group (CG—no injury), exercise group (EG—no injury with physical exercise), lesion group (LG—injury, but without exercise), and treated group (LEG—injury and physical exercise). LG and LEG were subjected to sciatic nerve compression with hemostat. From the 3rd day after injury began treatment with exercise, and after 22 days occurs the removal of a nerve fragment for morphological analysis. Results. Regarding allodynia, CG obtained values less than EG (p = 0.012) and larger than LG and LEG (p < 0.001). Histological results showed that CG and EG had normal appearance, as LG and LEG showed up with large amounts of inflammatory infiltration, degeneration and disruption of nerve fibers, and reduction of the myelin sheath; however LEG presented some regenerated fibers. From the morphometric data there were significant differences, for nerve fiber diameter, comparing CG with LG and LEG and comparing axon diameter and the thickness of the myelin of the CG to others. Conclusion. Climb stairs resistance exercise was not effective to speed up the regenerative process of axons.

Ganho de extensibilidade dos músculos isquiotibiais comparando o alongamento estático associado ou não à crioterapia

INTRODUCTION: Cryotherapy is indicated to decrease the discomfort during the sessions of stretching and provide better results in the gain of extensibility. OBJECTIVE: Studys objective was to verify the gain extensibility of ham-string muscles, comparing the static stretching with static stretching associated with cryotherapy. MATERIALS AND METHODS: Twenty subjects participated of the study, aged between 18 and 25 years, divided equally in two groups, considering that both performed stretching and stretching associated with cryotherapy. It has been used ice packs on the back of the thigh for 15 minutes. Then, it has been realized the application of static stretching of hamstrings, in two sets of 30 seconds each. RESULTS: It has been found significant differences in the extensibility of hamstring muscles in both groups, but there were no significant differences in the extensibility of hamstring muscles when compared static stretching with static stretching associated with cryotherapy. CONCLUSION: There was an immediate increase of the extensibility of hamstring muscles. However, the use of cryotherapy was not effective for gain of extensibility.

Effects of three protocols of hamstring muscle stretching and paravertebral lumbar

Introduction the muscle stretching is widely used to gain extensibility and flexibility, it is important to know the duration of these effects, after return to usual activity level. Thus, the aim of this study was to analyze the effect of three protocols of hamstring and paravertebral lumbar muscles stretching, and joint flexibility and muscle extensibility after six weeks. Methods participants were 40 volunteers, with limited hamstring extensibility, randomized into three groups: active stretching static (n = 14), proprioceptive neuromuscular facilitation (n = 14) and kinesiostretching (n = 12). The protocol was divided into 3 stages: the 1st control (six weeks), the 2nd application of stretch (six weeks) and the 3rd follow-up (eight weeks). The project was approved by the Ethics Committee on Human Research Unioeste, under protocol number 25536/2008. Four evaluations were conducted with board coupled to a system and goniometry and Well´s bench, distributed at the beginning and end of each step. Data were analyzed with repeated measures ANOVA, and one-way, with a significance level of 5%. Results there was no significant difference for the three groups in the control stage. There were significant differences in the three protocols in the stage of stretching. After follow-up stage, there was significant difference in the ratings to the board goniometry, and there was no difference in the Well’s Bench. Conclusion the three techniques promoted significant gain in extensibility and flexibility, extensibility was not maintained after the follow-up stage, and the flexibility of the posterior chain continued gains.

Análise do tecido muscular de ratos Wistar submetidos ao modelo de compressão do nervo isquiático e à crioterapia

RESUMO Objetivo: Avaliar os efeitos da compressao nervosa do isquiatico direito e da crioterapia no tecido muscular. Metodos: Foram utilizados 42 ratos Wistar machos, subdivididos nos Grupos Controle, Lesao 3, Lesao 8 e Lesao 15, submetidos a compressao nervosa e eutanasiados, respectivamente, no 3°, 8° e 15° dias pos-operatorio; Lesao Crioterapia 3, tratado com crioterapia, por imersao durante 20 minutos, por 1 dia, e eutanasiados no 3° dia pos-operatorio; e Lesao Crioterapia 8 e Lesao Crioterapia 15, tratados durante 6 [...]ABSTRACT Objective: To evaluate the effects of right sciatic nerve compression and cryotherapy on muscle tissue. Methods: We used 42 male Wistar rats, subdivided in the following Groups Control, Injury 3, Injury 8 and Injury 15 submitted to nerve compression and euthanized in the 3rd, 8th and 15th day after surgery. The Cryotherapy Injury 3 was entailed treatment with cryotherapy by immersion of the animal in recipient for 20 minutes during 1 day, then animals were euthanized at the 3rd day after surgery, and the Cryotherapy Injury 8 and the Cryotherapy Injury 15 was treated for 6 days, and euthanized at the 8th and 15th day after surgery. Functional evaluation was performed by the grasping strength of the right pelvic limb. The right tibialis anterior muscles were evaluated for mass, smaller diameter and cross-sectional area. In the Cryotherapy Injury 8 and the Cryotherapy Injury 15 groups, the hydroxyproline was dosed in the right soles. Results: In the compression there was a significant difference in the Injury Groups compared with the Control Group (p<0.05). In the smaller diameter, the compression in Control Group was higher than Injury 8 (p=0.0094), Injury 15 (p=0.002) and Cryotherapy Injury 15 (p<0.001) groups. The comparison between groups with euthanasia in the same post-operative period, a significant difference (p=0.0363) was seen in day 8th after surgery, and this result in Cryotherapy Injury Group was greater than Injury Group. In the fiber area, Control Group was also higher than the Injury 8 (p=0.0018), the Injury 15 (p<0.001) and the Cryotherapy Injury 15 (p<0.001). In hydroxyproline, no significant difference was seen between groups. Conclusion: Nerve damage resulted in decreased muscle strength and trophism, the cryotherapy delayed hypotrophy, but this effect did not persist after cessation of treatment.

Evaluation of nociception and edema in experimental sciatic nerve compression model in Wistar rats treated with cryotherapy

JUSTIFICATIVA E OBJETIVOS: Considerando a carencia de estudos a respeito da crioterapia como tratamento de lesao nervosa periferica, este estudo teve como objetivo avaliar a nocicepcao e o edema em modelo experimental de compressao do nervo isquiatico em ratos Wistar, tratados com crioterapia. METODOS: Foram utilizados 42 ratos separados aleatoriamente nos grupos: G1 - controle, eutanasiado no 15o de pos-operatorio; G2, G3 e G4 - submetidos a compressao do nervo isquiatico, eutanasiados no 3o, 8o e 15o de pos-operatorio, respectivamente; G5, G6 e G7 - submetidos a compressao do nervo isquiatico e tratados com crioterapia, eutanasiados no 3o, 8o e 15o de pos-operatorio, respectivamente. As avaliacoes da nocicepcao no local da lesao e na regiao plantar, e a verificacao do edema aconteceram nos momentos pre-lesao, 2o dia de pos-operatorio e no dia correspondente a eutanasia de cada grupo lesionado. A analise estatistica foi pela ANOVA mista, com nivel de significância de 5%. RESULTADOS: No local da lesao houve diminuicao do limiar doloroso nos grupos lesionados, entretanto, apresentou aumento do mesmo nos grupos relacionados as avaliacoes do 8o e 15o de pos-operatorio. Ja a nocicepcao realizada na regiao plantar foi semelhante a anterior, porem o limar nao aumentou. Com relacao ao edema, houve diferenca significativa na avaliacao realizada no 3o e no 2o dias de pos-operatorio correspondentes ao G1, G4 e G7, que apresentaram valores maiores comparadas com a pre-lesao. CONCLUSAO: A crioterapia nao auxiliou no aumento do limiar nociceptivo e reducao de edema.