Lou Zhiyong

Crystallization and preliminary X-ray crystallographic analysis of human geranylgeranyl pyrophosphate synthase

Abstract Human geranylgeranyl pyrophosphate synthase (GGPS) is an enzyme that catalyzes the synthesis of geranylgeranyl pyrophosphate (GGPP) from farnesyldiphosphate and isopentenyldiphosphate. Reconbinant human GGPS was crystallized by the hanging-drop vapor diffusion method. Crystals were grown at 18°C using PEG 4000 as precipitant. Diffraction data were obtained to a resolution of 2.8xa0A from a single frozen crystal belonging to space group P1, with unit-cell parameters: a = 68.9xa0A, b = 107.7xa0A, c = 137.4xa0A, a = 99.6°, β = 97.6°, γ = 97.8°. * Supported by the National High-Tech Project (Grant No. 2002BA711A12), the National Basic Research Development Programs of China Project (Grant No. G1999075602) and National Natural Science Foundation of China (Grant No. 30221003)