Rao Zihe
Tsinghua University
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Publication
Featured researches published by Rao Zihe.
Chinese Science Bulletin | 2001
Hongzhen He; Yu Shao; Chen Yuhang; Liu Bingbing; Cao Zhenbo; Jiang Fan; Liu Yi-wei; Tang Hong; Liu Jinyuan; Zhao Nanming; Rao Zihe
In order to obtain phase information for the X-ray diffraction of tabtoxin resistance protein (TTR) crystal using the MAD phasing method, a selenomethionine (Se-Met) derivative of TTR was overexpressed inE. coli strain M15, with pQE-30 plasmid, through IPTG induction in M9 medium containing Se-Met. The product was purified to an estimated homogeneity of greater than 95% according to SDS-PAGE, by a Ni-NTA metal affinity followed by a Mono Q anion exchange column chromatography. The successful substitution of Se-Met for methionine (Met) was confirmed by MALDI-TOF and ESI-Quadrupole Mass Spectrometry analysis. The derivative crystal was obtained using similar conditions as those for the native.
Archive | 2012
Shang Luqing; Xu Mengying; Lou Zhiyong; Wang Yaxin; Zhao Qiang; Zhao Xiangshuai; Yang Cheng; Yin Zheng; Rao Zihe
Archive | 2013
Rao Zihe; Yang Cheng; Liu Dazhi; Wu Tingting; Liu Wei; Fu Sheng; Li Shuang; Wang Xingang; Yu Fei; Liu Xiang
Archive | 2013
Rao Zihe; Yang Cheng; Lou Zhiyong; Guo Yu; Zhou Honggang; Chen Weiqiang; Zhang Tanjie; Tang Yanting; Li Jinnan; Feng Jinlei
Archive | 2013
Yin Zheng; Shang Luqing; Zhao Xiangshuai; Lou Zhiyong; Wang Yaxin; Xu Mengying; Wang Peng; Cui Cancan; Chen Cheng; Zhao Qiang; Zhou Honggang; Yang Cheng; Rao Zihe
Archive | 2013
Cui Qingxin; Lou Zhiyong; Ma Ming; Rao Zihe; Sun Yuna; Xue Fei
Archive | 2013
Rao Zihe; Yang Cheng; Zhou Honggang; Tang Yanting; Sun Yutao; Feng Jinlei; Zhang Tanjie; Li Jinnan; Chen Weiqiang; Wang Peng
Archive | 2013
Rao Zihe; Yang Cheng; Zhou Honggang; Wang Lijun; Zhang Ying; Dong Hui; Wang Zhongling; Chang Zhenying; Yao Weili
Archive | 2013
Rao Zihe; Yang Cheng; Li Jinnan; Zhang Tanjie; Feng Jinlei; Tang Yanting; Peng Chun E; Chen Weiqiang
Archive | 2013
Rao Zihe; Yang Cheng; Bai Cuigai; Chen Yue; Dai Dongfang; Jin Bingde; Zhang Wei; Wang Song; Li Shuang; Wang Xingang
