Louis A. Kamentsky
Columbia University
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Featured researches published by Louis A. Kamentsky.
Science | 1965
Louis A. Kamentsky; Myron R. Melamed; Herbert Derman
A new device has been developed for measuring and displaying multiple spectrophotometric properties of biological cells at rates exceeding 500 cells per second. Preliminary observations of human cells from different sites in the body were made at wavelengths of 2537 and 4100 angstroms to estimate cellular nucleic acid per unit volume of individual cells of large populations of cells. Display patterns were obtained which were consistent, and characteristically different for certain of the cell populations studied.
Acta Cytologica | 1997
Louis A. Kamentsky; Douglas E. Burger; Russell J. Gershman; Lee D. Kamentsky
OBJECTIVE To show that laser scanning cytometry (LSCM) can provide data equivalent to flow cytometry (FCM) data and furnish a number of benefits, including cell relocation for visualization and several additional measurement features that may make it more suitable than FCM for pathology laboratories. STUDY DESIGN A laser scanning cytometer, the LSC, was developed. Several instruments, at sites in the United States and Japan during the last two years, provided data characterizing the instrument and its usefulness. RESULTS Data describing the sensitivity, precision, accuracy, utility of added measurement features and cell relocation capabilities of the LSC are presented. The data illustrate the applicability of the LSC to multiparameter DNA ploidy studies, resolution of phases of the cell cycle and cytogenetics. CONCLUSION Because it is microscope based and measures cells on a slide, not in a flow chamber; records the position of each cell on the slide; and has higher resolution, LSCM provides a number of benefits that may make it more suitable than FCM for pathology laboratories.
Methods in Cell Biology | 2001
Louis A. Kamentsky
Publisher Summary Laser scanning cytometry (LSCM) automatically measures laser excited fluorescence at multiple wavelengths and light scatter from cells on slides that have been treated with one or more fluorescent dyes to rapidly determine multiple cellular constituents and other features of the cells. This chapter describes a specific laser scanning cytometer, the LSC that can use these techniques perfected for flow cytometry (FCM) to provide data comparable to FCM. Because it is microscope based and measures cells on the surface of a slide, records position of each cell on the slide, and has higher resolution, it can provide a number of benefits that may make it a more suitable cytometer for certain applications. LSCM is not comparable to confocal microscopy. Because LSCM must uniformly illuminate cells throughout their volume to obtain accurate whole cell constituent measurements, its optical components are designed to be nonconfocal. LSCM uses large field depths, and confocal microscopy emphasizes short field depth to provide detailed images at a narrow depth focal plane through each cell that is imaged. Additionally, LSCM is designed to automatically measure large heterogeneous populations of cells, unlike the detailed single cell analysis, for which confocal microscopy is most useful.
Science | 1967
Louis A. Kamentsky; Myron R. Melamed
A new device can physically separate cells of predetermined optical properties, from large populations of cells in suspension.
Cytometry | 1996
Louis A. Kamentsky
A microscope-based laser scanning cytometer (LSCM) has been developed that automatically measures multiple wavelength fluorescence and light scattering of cells on a microscope slide and generates lists of cytochemical and morphological features for each of thousands of cells in a typical sample. For a sample stained with a DNA stain, among the features generated are the value (DNA content), peak (chromatin condensation), and area (nuclear size), as well as the location of the cell on the slide. When combined with each other, these features give detailed resolution of the mammalian cell cycle, including the separation of mitotic from interphase cells. This is demonstrated under a variety of conditions, including cells that were fixed while in suspension and then adhered to a microscope slide, cytocentrifuge preparations, adherent cells fixed in situ on a microscope slide, on viable adherent cells, and on pathological tissue material. Galleries are shown of images of cells that were identified by the instrument as belonging to specific stages of the cell cycle, based on their biochemical staining, and were automatically relocated for viewing. The images are either epifluorescence images of the cells stained with the DNA fluorochrome or brightfield images of cells from slides that were restained with chromatic dyes.
Laboratory Automation & Information Management | 1997
Mark L. Weissman; Louis A. Kamentsky; Lee D. Kamentsky
A computerized specimen encoder for use with microscope analysis and pathological studies. The slide encoder is attached to the movable microscope stage, whereby X-Y plane movement and location, is correlated to examination of a specimen on an identified slide, with information marking and location being directly correspondingly written on computer storage media, during the examination. The information marking is in the form of computer generated indicia which are placed at a computer image location of the slide at predetermined time intervals. Subsequent use of the computer-stored information, coupled with the slide encoder, in a slide re-examination, permits independent retrieval of such information and location on the slide. The encoder device is provided with a grayscale marker which marks in varying shades of gray, ranging from white to black, the time spent by a slide screener on a particular portion of the specimen and the number of times spent viewing a particular portion of the specimen.
Cytometry | 1997
Louis A. Kamentsky; Lee D. Kamentsky; Jonathan A. Fletcher; Akira Kurose
Multiparameter laser scanning cytometry has been applied to the automatic counting of probe spots and the simultaneous measurement of cellular DNA for fluorescence in situ hybridization (FISH) prepared specimens counterstained with propidium iodide. Relatively low resolution imaging, highly variable probe fluorescence, spectral overlap of probe with counterstain fluorescence, and autofluorescence required the development of an image processing method to detect and isolate FISH probe spots. Inability to properly apportion detected probe spots because of overlapping probe spot images in the same cell required development of a method to eliminate cell data whenever spots in that cell could not be reliably isolated. Laser scanning cytometry incorporating these methods to determine per cell probe spot count and DNA is demonstrated on tissue cultures and peripheral blood cells using different centromeric FISH probes with either FITC or Spectrum Green labeling.
Science | 1969
Myron R. Melamed; Louis A. Kamentsky; Edward A. Boyse
An appropriately modified cell spectrophotometer was used successfully for performing automatic counts of live and dead cells in the cytotoxic test, with trypan blue staining as an indicator of dead cells and light scattering to identify viable cells.
Methods in Cell Biology | 2004
Louis A. Kamentsky; Melvin Henriksen; Elena Holden
Publisher Summary Flow cytometry (FC) has been at the forefront of quantitative cytometric analysis. Recent experimental needs in the life sciences demand a combination of quantitative cytometry and imaging cytometry. This demand has been fulfilled by the development of laser scanning cytometry (LSC). LSC is a combination of quantitative cytometry and imaging cytometry. LSC technology transforms the microscope from a qualitative to a quantitative tool for cell biology. Laser scanning cytometer and two newer, next-generation systems, the automated imaging cytometer (iCyte), and the research imaging cytometer (iCys) are a product line of laser scanning cytometers. The iCys and iCyte systems provide for either interactive (iCys) or walkaway (iCyte) analysis. The chapter gives an overview of obtaining the images; segmentation and feature extraction; and data analysis for these cytometers. The iNovator application development module adds significantly to the capabilities of the iCyte and iCys systems. With the iNovator, the user can (1) employ imaging tools to the segmentation and data analysis process, (2) control the process with visually oriented macros, and (3) perform multiscale scanning and analysis. The user has the ability to define and save numerous types of data files, both numerical and image. A number of applications have been developed for the new iCys and iCyte platforms.
Cancer | 1972
Myron R. Melamed; Adams Lr; F. Traganos; A. Zimring; Louis A. Kamentsky
The green nuclear and red cytoplasmic fluorescence of individual leukocytes in whole blood stained with acridine orange can be measured rapidly and simultaneously at the two wave lengths of maximum emission by use of a flow‐through cytophotometer. Prior studies on normal individuals showing consistent differences in the amplitude of red emission from lymphocytes, monocytes, and granulocytes have been extended here to investigate patients with leukemia, lymphoma, and a variety of other neoplasms. The measurements obtained are presented and discussed. Both nuclear and cytoplasmic fluorescence of leukemic cells was found to vary greatly when compared with normal leukocytes, except in the case of chronic lymphocytic leukemia. Sequential examinations of blood from leukemia patients under treatment show very striking changes due to the treatment, with measurements returning to normal as the leukemia goes into remission. Most patients with lymphomas, carcinomas, and other neoplasms had essentially normal patterns of measurement, but, in a few cases, there were cells with increased amplitude of green fluorescence that could have resulted from hyperdiploid or nucleic acid synthesizing cells in the peripheral blood. It is not known whether these represented tumor cells or immature cells from the marrow.