Lubica Majtanova

Honeydew honey as a potent antibacterial agent in eradication of multi‐drug resistant Stenotrophomonas maltophilia isolates from cancer patients

Multi‐drug resistance in nosocomial pathogens is a continually evolving and alarming problem in health care units. Since ancient times, honey has been used successfully for the treatment of a broad spectrum of infections with no risk of resistance development.

Increasing trend of resistance to nalidixic acid and emerging ceftriaxone and ciprofloxacin resistance in Salmonella enterica serovar Typhimurium in Slovakia, 2005 to 2009

A 5-year survey from 2005 to 2009 revealed an increasing trend of resistance to nalidixic acid (from 0% in 2005 to 11% in 2009) among 858 clinical isolates of Salmonella Typhimurium. In addition, 10 ceftriaxone-resistant and 3 ciprofloxacin-resistant Salmonella Typhimurium isolates were detected over the period of study.

Postantibiotic effect of some antibiotics on the metabolism of Pseudomonas aeruginosa.

The influence of the postantibiotic effects (PAEs) of ciprofloxacin, pefloxacin, imipenem, meropenem and amikacin in the suprainhibitory concentrations (2 × and 4 × MIC) on the metabolic processes of P. aeruginosa was studied. The synthesis of macromolecules was expressed by influencing of the incorporation rate of [14C] adenine and [14C] leucine. Remarkable affecting of both biosynthetic processes evoked the suprainhibitory concentration 4 × MIC of meropenem by inhibition of the nucleic acids synthesis to 76.1% and proteins synthesis to 61.1% against the control. The suprainhibitory concentration 4 × MIC of both pefloxacin and ciprofloxacin affected the highest suppression of the endogenous respiration to 16.5% and to 20.3%, respectively. The respiration was influenced the least after the effect of meropenem in the both suprainhibitory concentrations tested. According to our knowledge, this is first report about the evaluation of the endogenous respiration after PAE. In this study we demonstrated the inhibitory effects of 4 × MIC concentration of antibiotics studied on the metabolic processes of P. aeruginosa. The results suggest a multiple mechanism for the PAE.

Trends in phage types of Salmonella enterica serovars Enteritidis and Typhimurium isolated in Slovakia from 1995 to 2009

The phage typing of 3900 isolates of Salmonella Enteritidis and 1741 isolates of Salmonella Typhimurium has been carried out in the period 1995-2009. Among Salmonella Enteritidis in individual years, the most prevalent phage type (PT) was 8. The most predominant PTs of Salmonella Typhimurium were DT104 and U302.

Phenotypic and Molecular Characterization of Human Salmonella enterica serovar 4,[5],12:i:- Isolates in Slovakia

Forty-three epidemiologically unrelated emerging Salmonella enterica subsp. enterica serovar 4,[5],12:i:- strains isolated during the period 2009–2010 in Slovakia were characterized by phenotypic and genotypic methods. Thirty-one isolates (72.1%) expressed resistance to ampicillin, streptomycin, sulfizoxazole, and tetracycline [R-type ASSuT]. The majority of the strains belonged to both definitive phage types DT193 (30.2%) and U311 (27.9%). Other phage types identified were U302 (6.9%), DT18 (4.7%), and DT194 (2.3%). Twelve strains (27.9%) were not typeable. Pulsed-field gel electrophoresis analysis identified three closely related major banding profiles (X1, X1a, and X2), suggesting the similarity and close epidemiological relationship between S. enterica serovar 4,[5],12:i:- strains. In two isolates with R-type ASSuT, phage type NT and in one isolate with R-type ACROSSuSxTTTMPNA, phage type DT193 class 1 integrons were found encoding blaPSE-1 and dfrA, aadA1, respectively.