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Featured researches published by Luc Marcellin.


Journal of Clinical Oncology | 1996

Loss of heterozygosity of the RB gene is a poor prognostic factor in patients with osteosarcoma.

Olivier Feugeas; Nathalie Guriec; Annie Babin-Boilletot; Luc Marcellin; Patrick Simon; S. R. Babin; Antoine Thyss; Patrick Hofman; Philippe Terrier; Chantal Kalifa; Maud Brunat-Mentigny; Louis-Marie Patricot; Francis Oberling

PURPOSE The usual therapy of osteosarcoma is neoadjuvant chemotherapy, followed by surgery, then by postoperative chemotherapy. There is no prognostic factor to predict, at diagnosis, the histologic response and final outcome. Inactivation of the retinoblastoma-susceptibility gene RB is associated with the pathogenesis of several human cancers. In primary osteosarcomas, loss of heterozygosity (LOH) at the RB locus has been found in greater than 60% of cases. The aim of this study was to determine the potential early prognostic value of LOH of RB gene on the biopsy material at diagnosis. PATIENTS AND METHODS Forty-seven patients with primary osteosarcoma, treated in four French institutions, were studied. LOH was studied by polymerase chain reaction (PCR) of an informative RB DNA polymorphism. RESULTS Assessment of LOH at the RB gene could be completed on 34 heterozygous patients only. LOH was found in 24 cases (70%). The event-free survival (EFS) rate at 60 months is 100% for patients without LOH, 43% for all patients with RB LOH, and 65% for nonmetastatic patients with RB LOH. The difference in EFS is highly significant at P = .008 and P = .024, respectively. Histologic response after preoperative chemotherapy did not show significant correlation with LOH status. CONCLUSION RB gene LOH appears to be an early predictive feature for osteosarcomas that indicates a potential unfavorable outcome. RB LOH study might shortly help to identify high-risk patients earlier. If this is verified, therapy could then be adapted earlier to the individuals real risk of relapse.


Journal of Clinical Oncology | 2005

Prognostic Significance of Allelic Imbalance at the c-kit Gene Locus and c-kit Overexpression by Immunohistochemistry in Pediatric Osteosarcomas

Natacha Entz-Werle; Luc Marcellin; Marie-Pierre Gaub; Eric Guerin; Anne Schneider; Perrine Berard-Marec; Chantal Kalifa; Laurence Brugiere; Hélène Pacquement; Claudine Schmitt; Marie-Dominique Tabone; Corinne Jeanne-Pasquier; Philippe Terrier; Frédérique Dijoud; Pierre Oudet; Patrick Lutz; Annie Babin-Boilletot

PURPOSE Since the recent development of biologic agents targeting oncogenes, increasing attention has been focused on determining the role of tyrosine kinase receptors in the pathogenesis of tumors. Our study was designed to investigate the status of region 4q12, which contains the candidate gene c-kit, and the expression of c-kit by immunohistochemistry (IHC). PATIENTS AND METHODS Paired blood and biopsy specimens of 68 children treated for high-grade primary osteosarcomas were collected. Microsatellite analysis at two genomic sites containing c-kit gene was performed on paired DNA using a sensible fluorescent polymerase chain reaction technology. To confirm the DNA data, we studied c-kit protein expression by IHC in 56 available paraffin-embedded tumor tissues. RESULTS The frequency of allelic imbalance (AI) at locus 4q12 was 39% in the overall population. In agreement with previous studies, we did not detect microsatellite instability, allowing us to hypothesize that this pathway is not implicated. Furthermore, the normal status at locus 4q12 was associated with a significantly better survival in the whole osteosarcoma population (P = .05). IHC overexpression of c-kit was concordant in all cases presenting an AI. However, normal status at locus 4q12 was correlated to an absence of c-kit protein expression in 19 (65.5%) of 29 informative cases. CONCLUSION Allelotyping of locus 4q12, which contains the c-kit gene, could help pediatric osteosarcoma prognostic screening and showed a strong correlation with overexpression of c-kit protein. These results allowed us to hypothesize that, in some cases, a mutation of c-kit gene could lead to a protein overexpression.


European Journal of Medical Genetics | 2014

Next-generation sequencing (NGS) as a fast molecular diagnosis tool for left ventricular noncompaction in an infant with compound mutations in the MYBPC3 gene

Elise Schaefer; Pauline Helms; Luc Marcellin; Philippe Desprez; Philippe Billaud; Valérie Chanavat; Robert Rousson; Gilles Millat

Left ventricular noncompaction (LVNC) is a clinically heterogeneous disorder characterized by a trabecular meshwork and deep intertrabecular myocardial recesses that communicate with the left ventricular cavity. LVNC is classified as a rare genetic cardiomyopathy. Molecular diagnosis is a challenge for the medical community as the condition shares morphologic features of hypertrophic and dilated cardiomyopathies. Several genetic causes of LVNC have been reported, with variable modes of inheritance, including autosomal dominant and X-linked inheritance, but relatively few responsible genes have been identified. In this report, we describe a case of a severe form of LVNC leading to death at 6 months of life. NGS sequencing using a custom design for hypertrophic cardiomyopathy panel allowed us to identify compound heterozygosity in the MYBPC3 gene (p.Lys505del, p.Pro955fs) in 3 days, confirming NGS sequencing as a fast molecular diagnosis tool. Other studies have reported neonatal presentation of cardiomyopathies associated with compound heterozygous or homozygous MYBPC3 mutations. In this family and in families in which parental truncating MYBPC3 mutations are identified, preimplantation or prenatal genetic screening should be considered as these genotypes leads to neonatal mortality and morbidity.


European Journal of Human Genetics | 2005

Trisomy 7 mosaicism, maternal uniparental heterodisomy 7 and Hirschsprung's disease in a child with Silver–Russell syndrome

Elisabeth Flori; Emmanuelle Girodon; Brigitte Samama; François Becmeur; Brigitte Viville; Françoise Girard-Lemaire; Bérénice Doray; Caroline Schluth; Luc Marcellin; Nelly Boehm; Michel Goossens; Veronique Pingault

Prenatal trisomy 7 is usually a cell culture artifact in amniocytes with normal diploid karyotype at birth and normal fetal outcome. In the same way, true prenatal trisomy 7 mosaicism usually results in a normal child except when trisomic cells persist after birth or when trisomy rescue leads to maternal uniparental disomy, which is responsible for 5.5–7% of patients with Silver–Russell syndrome (SRS). We report here on the unusual association of SRS and Hirschsprungs disease (HSCR) in a patient with maternal uniparental heterodisomy 7 and trisomy 7 mosaicism in intestine and skin fibroblasts. HSCR may be fortuitous given its frequency, multifactorial inheritance and genetic heterogeneity. However, the presence of the trisomy 7 mosaicism in intestine as well as in skin fibroblasts suggests that SRS and HSCR might possibly be related. Such an association might result from either an increased dosage of a nonimprinted gene due to trisomy 7 mosaicism in skin fibroblasts (leading to SRS) and in intestine (leading to HSCR), or from an overexpression, through genomic imprinting, of maternally expressed imprinted allele(s) in skin fibroblasts and intestine or from a combination of trisomy 7 mosaicism and genomic imprinting. This report suggests that the SRS phenotype observed in maternal uniparental disomy 7 (mUPD(7)) patients might also result from an undetected low level of trisomy 7 mosaicism. In order to validate this hypothesis, we propose to perform a conventional and molecular cytogenetic analysis in different tissues every time mUPD(7) is displayed.


Breast Cancer Research and Treatment | 1997

Cd44 isoforms with exon v6 and metastasis of primary N0M0 breast carcinomas

Nathalie Guriec; Béatrice Gairard; Luc Marcellin; Astrid Wilk; Hubert Caldéroli; Robert Renaud; Jean Pierre Bergerat; Francis Oberling

New isoforms of CD44 with alternatively spliced exons have recently been described. Expression of exon v6 seems to be of particular interest. It has indeed been associated with poorer outcome of breast cancer patients with node invasion at diagnosis. However, no data were available for patients N0M0 (with neither metastasis nor node invasion at diagnosis). Moreover, previous statistical analyses were realized using immunohistochemical methods to detect CD44v6 expression although several variants with exon v6 have been described. We investigated expression of isoforms containing CD44v6 using an RT-PCR approach and a panel of 25 normal breast specimens, 10 mammary fibroadenomas, 8 cystic samples and 52 primary breast tumors (38 invasive N0M0). Normal breasts, fibroadenomas, and cysts all express the same variant, A (with exon v6 only), while several transcripts are amplified in tumors. Expression of variants other than A correlates with acquisition of a malignant phenotype. Invasive cancers also express additional variants in comparison with in situ carcinomas. Metastasis capacities seem to be associated with transcription of variants other than A but also with no transcription of some of them, variants D (with exons v6 and v10) and L (with exons v6 to v10). Expression of variants D and L correlates with higher percentages of disease-free survival and better outcome. Expression of CD44 splice variants with exon v6, as detected by RT-PCR, might be a useful prognostic factor for breast cancer. However, since the series size is small, our results need to be confirmed by later studies on a larger number of patients.


European Journal of Medical Genetics | 2012

Prenatal diagnosis of a 12q22q23.2 interstitial deletion by array CGH in a malformed fetus.

Valérie Kremer; Françoise Girard; Bernard Gasser; Luc Marcellin; Dominique Christmann; Israel Nisand; Evelyne Schmitt; Sylvie Florent; Elisabeth Flori

We report the prenatal diagnosis of a 12q22q23.2 de novo interstitial deletion performed by array based comparative genomic hybridization (array CGH) in a fetus with cystic hygroma colli, intrauterine growth retardation, microcephaly and micrognathism. Haploinsufficiency for insuline-like growth factor 1 gene (IGF1), which is mapped in the deleted region, is suggested because of its implication in prenatal and postnatal growth and in neuronal maturation. This case demonstrates the contribution of array CGH in prenatal diagnosis for detecting small unbalanced chromosomal abnormalities in malformed fetuses and, subsequently, for genetic counselling.


Journal of Pediatric Surgery | 2003

The urinary bladder: An extremely rare location of pediatric neuroblastoma.

Natacha Entz-Werle; Luc Marcellin; François Becmeur; Didier Eyer; Annie Babin-Boilletot; Patrick Lutz


Archives De Pediatrie | 2003

Néphroblastome en sablier : une cause inhabituelle de compression médullaire

N. Cojean; Natacha Entz-Werle; Didier Eyer; François Becmeur; Pierre Kehrli; Luc Marcellin; Dominique Christmann; Babin A; Patrick Lutz


Archives De Pediatrie | 2003

[Dumbbell nephroblastoma: an uncommon cause of spinal cord compression].

N. Cojean; Natacha Entz-Werle; Didier Eyer; François Becmeur; Pierre Kehrli; Luc Marcellin; Dominique Christmann; Babin A; Patrick Lutz


Pediatric Blood & Cancer | 2004

A very uncommon presentation of bilateral Wilms tumor

Natacha Entz-Werle; Joelle Terzic; Luc Marcellin; Dominique Christmann; Annie Babin-Boilletot; Michel Fishbach; Paul Sauvage; Patrick Lutz

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Pierre Kehrli

University of Strasbourg

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Patrick Lutz

Memorial Hospital of South Bend

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Antoine Thyss

University of Nice Sophia Antipolis

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