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Dive into the research topics where Luciana Prado Maia is active.

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Featured researches published by Luciana Prado Maia.


Revista De Saude Publica | 2006

Heterocontrole da fluoretação da água de abastecimento público em Bauru, SP, Brasil

Irene Ramires; Luciana Prado Maia; Daniela dos Santos Rigolizzo; José Roberto Pereira Lauris; Marília Afonso Rabelo Buzalaf

OBJETIVO: Avaliar a fluoretacao da agua de abastecimento publico, comparando os resultados obtidos com dados previos de fluoretacao. METODOS: O estudo foi realizado de marco de 2004 a 2005, na cidade de Bauru, Estado de Sao Paulo. Mensalmente, em datas estabelecidas por sorteio, foram coletadas 60 amostras de agua nos 19 setores de abastecimento, totalizando 737 amostras. A concentracao de fluor presente nas amostras de agua foi determinada em duplicata, utilizando-se o eletrodo ion sensivel (Orion 9609), acoplado ao potenciometro. Uma vez analisadas, as amostras foram classificadas como aceitaveis ou inaceitaveis de acordo com a concentracao de fluor. A analise estatistica empregada foi do tipo descritiva. RESULTADOS: A concentracao media de fluor observada nos diferentes meses de coleta variou entre 0,37 e 1,00 mg/l. Cerca de 85% das amostras foram classificadas como aceitaveis. CONCLUSOES: Comparados com dados de estudos previos realizados na cidade, foi observada uma melhoria nas condicoes de fluoretacao da agua de abastecimento, um ano apos a implantacao do heterocontrole. A implantacao do monitoramento da fluoretacao da agua de abastecimento por sistemas de vigilância deve ser incentivada, sendo fundamental para o controle da carie dentaria.


Caries Research | 2009

Environmental and Individual Factors Associated with Nail Fluoride Concentration

R. Fukushima; Daniela dos Santos Rigolizzo; Luciana Prado Maia; F.C. Sampaio; José Roberto Pereira Lauris; M. A. R. Buzalaf

Nails have been suggested as suitable biomarkers of exposure to F, with the advantage of being easily obtained. The effect of water F concentration, age, gender, nail growth rate and geographical area on the F concentration in the fingernail and toenail clippings were evaluated. Volunteers (n = 300) aged 3–7, 14–20, 30–40 and 50–60 years from five Brazilian communities (A–E) participated. Drinking water and nail samples were collected and F concentration was analyzed with the electrode. A reference mark was made on each nail and growth rates were calculated. Data were analyzed by ANOVA and linear regression (α = 0.05). Mean water F concentrations (± SE, mg/l) were 0.09 ± 0.01, 0.15 ± 0.01, 0.66 ± 0.01, 0.72 ± 0.02, and 1.68 ± 0.08 for A–E, respectively. Mean F concentrations (± SE, mg/kg) ranged between 1.38 ± 0.14 (A, 50–60 years) and 10.20 ± 2.35 (D, 50–60 years) for fingernails, and between 0.92 ± 0.08 (A, 14–20 years) and 7.35 ± 0.80 (E, 50–60 years) for toenails. Among the tested factors, geographical area and water F concentration exerted the most influence on finger- and toenail F concentrations. Subjects of older age groups (30–40 and 50–60 years) from D and E showed higher nail F concentrations than the others. Females presented higher nail F concentration than males. Water F concentration, age, gender and geographical area influenced the F concentration of finger- and toenails, and hence should be taken into account when using this biomarker of exposure to predict risk for dental fluorosis.


Brazilian Dental Journal | 2010

Evaluation of in vitro human gingival fibroblast seeding on acellular dermal matrix

Annelissa Zorzeto Rodrigues; Paulo Tambasco de Oliveira; Arthur B. Novaes; Luciana Prado Maia; Sérgio Luís Scombatti de Souza; Daniela B. Palioto

The acellular dermal matrix (ADM) was introduced in periodontology as a substitute for the autogenous grafts, which became restricted because of the limited source of donors tissue. The aim of this study was to investigate, in vitro, the distribution, proliferation and viability of human gingival fibroblasts seeded onto ADM. ADM was seeded with human gingival fibroblasts for up to 21 days. The following parameters were evaluated: cell distribution, proliferation and viability. Results revealed that, at day 7, fibroblasts were adherent and spread on ADM surface, and were unevenly distributed, forming a discontinuous single cell layer; at day 14, a confluent fibroblastic monolayer lining ADM surface was noticed. At day 21, the cell monolayer exhibited a reduction in cell density. At 7 days, about to 90% of adherent cells on ADM surface were cycling while at 14 and 21 days this proportion was significantly reduced. A high proportion of viable cell was detected on AMD surface both on 14 and 21 days. The results suggest that fibroblast seeding onto ADM for 14 days can allow good conditions for cell adhesion and spreading on the matrix; however, migration inside the matrix was limited.


Clinical Implant Dentistry and Related Research | 2015

Influence of Periodontal Biotype on Buccal Bone Remodeling after Tooth Extraction Using the Flapless Approach with a Xenograft: A Histomorphometric and Fluorescence Study in Small Dogs

Luciana Prado Maia; Danilo Maeda Reino; Arthur B. Novaes; Valdir Antonio Muglia; Mário Taba; Grisi Mf; Sérgio Luís Scombatti de Souza; Daniela B. Palioto

BACKGROUND Several approaches have been used to counteract alveolar bone resorption after tooth extraction. PURPOSE The aim of the present study was to evaluate the influence of gingival thickness and bone grafting on buccal bone remodeling in extraction sockets with thin buccal bone, using a flapless approach. MATERIALS AND METHODS The gingiva of 8 dogs was thinned at one side of the mandible and mandibular premolars were extracted without flaps. The sockets were randomly assigned to the test group (thin gingiva) (TG), the test group with grafting material TG + GM, the control group (normal gingiva) (CG), or the control group with grafting material CG + GM. Ground sections were prepared from 12-week healing biopsies, and histomorphometry and fluorescence analysis were performed. RESULTS In the groups with thin gingiva, numerically greater buccal bone loss was observed, while there were no differences between grafted and nongrafted sites. A numerically higher rate of mineralization was observed for the grafted sites, as compared with the nongrafted sites, at 12 weeks. CONCLUSIONS A thin buccal bone plate leads to higher bone loss in extraction sockets, even with flapless surgery. The gingival thickness or the use of a graft material did not prevent buccal bone resorption in a naturally thin biotype, but modified the mineralization process.


Brazilian Dental Journal | 2012

Treatment of Gingival Recessions in Heavy Smokers Using Two Surgical Techniques: A Controlled Clinical Trial

Danilo Maeda Reino; Arthur B. Novaes; Luciana Prado Maia; Daniela B. Palioto; Márcio Fernando de Moraes Grisi; Mário Taba; Sérgio Luís Scombatti de Souza

Smokers have small root coverage which is associated with bad vascularity of periodontal tissues. This study evaluated a technique that can increase the blood supply to the periodontal tissues compared with a traditional technique. Twenty heavy smokers (10 males and 10 females) with two bilateral Miller class I gingival recessions received coronally positioned flaps in one side (Control group)and extended flap technique in the other side (Test group). Clinical measurements (probing pocket depth, clinical attachment level, bleeding on probing, gingival recession height, gingival recession width, amount of keratinized tissue, and width and height of the papillae adjacent to the recession) were determined at baseline, 3 and 6 months postoperatively. Salivary cotinina samples were taken as an indicator of the nicotine exposure level. No statistically significant differences (p>0.05) were detected for the clinical measurements or smoke exposure. Both techniques promoted low root coverage (Control group: 43.18% and Test group: 44.52%). In conclusion, no difference was found in root coverage between the techniques. Root coverage is possible and uneventful even, if rather low, in heavy smoker patients with low plaque and bleeding indices.


Journal of Periodontology | 2011

In Vitro Evaluation of Acellular Dermal Matrix as a Three-Dimensional Scaffold for Gingival Fibroblasts Seeding

Luciana Prado Maia; Arthur B. Novaes; Sérgio Luís Scombatti de Souza; Márcio Fernando de Moraes Grisi; Mário Taba; Daniela B. Palioto

BACKGROUND Tissue engineering principles could improve the incorporation of acellular dermal matrix (ADM). The aim of this study is to verify if ADM is a suitable three-dimensional matrix for gingival fibroblasts and cancerous cells ingrowth, and also if cultured medium conditioned in ADM affect cellular behavior. METHODS Canine gingival fibroblasts (CGF), human gingival fibroblasts (HGF), and murine melanoma cell line (B16F10) were seeded on ADM for up to 14 days. The following parameters were assessed: morphology and distribution of CGF, HGF, and B16F10; CGF and HGF viability; and the effect of ADM conditioned medium (CM) on CGF viability. RESULTS Epifluorescence revealed that CGF were unevenly distributed on the ADM surface, showing no increase in cell number over the periods of study; HGF formed a monolayer on the ADM surface in a higher number at 14 days (P <0.05); B16F10 exhibited an increase in cell number within 7 days (P <0.05), and were mainly arranged in cell aggregates on the ADM, forming a continuous layer at 14 days. A higher percentage of cells on the ADM surface (P <0.05) compared to inside was observed for all cell types. 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) values indicated higher cell viability in samples cultured with HGF compared to CGF (P = 0.024). A significantly lower cell viability for CGF grown in CM compared to cells grown in non-CM was observed at 48 and 72 hours (P <0.05). CONCLUSIONS ADM is not suitable as a three-dimensional matrix for gingival fibroblasts ingrowth. Gingival fibroblasts and highly proliferative cells as B16F10 can only be superficially located on ADM, and CGF are negatively affected by culture medium conditioned in ADM, reducing its viability.


Journal of Clinical Periodontology | 2015

Influence of periodontal tissue thickness on buccal plate remodelling on immediate implants with xenograft

Luciana Prado Maia; Danilo Maeda Reino; Valdir Antonio Muglia; Adriana Luisa Gonçalves de Almeida; Antonio Nanci; Rima Wazen; Paulo Tambasco de Oliveira; Daniela B. Palioto; Arthur B. Novaes

AIM To evaluate the influence of gingival thickness and bone grafting on buccal bone plate remodelling after immediate implant placement in sockets with thin buccal bone, using a flapless approach. MATERIALS AND METHODS The gingiva of eight dogs was thinned at one side of the mandible, mandibular premolars were extracted without flaps, and four implants were installed on each side at 1.5 mm from the buccal bone. The sites were randomly assigned into: TG (test group) = thin gingiva; TG + GM (TG with grafting material); CG (control group) = normal gingiva; and CG + GM (CG with grafting material). After 12 weeks the dogs were sacrificed and the samples were processed for histological analysis. RESULTS All animals exhibited a thin buccal bone initially. In all the experimental groups the buccal gap was filled with newly formed bone and the buccal bone level was slightly apical to the implant shoulder. There were no statistically significant differences among the groups for the histomorphometric parameters. CONCLUSIONS The thickness of the buccal bone was a fundamental factor in buccal bone plate resorption, even with flapless implantation. The gingival thickness or the addition of a biomaterial in the gap did not influence the results.


Brazilian Dental Journal | 2013

Morphological, Functional and Biochemical Characterization of Canine Gingival Fibroblasts

Camila Bonvicino Pelegrini; Luciana Prado Maia; Sérgio Luís Scombatti de Souza; Mário Taba; Daniela B. Palioto

As dogs are good models for in vivo studies, it is interesting to evaluate the behavior of canine gingival fibroblasts (CGF) in vitro, so that these cells could be seeded on a matrix and later studied in vivo. The aim of this study was to perform a morphological, functional and biochemical analysis of CGF, comparing it with human gingival fibroblasts (HGF), as well as to evaluate the change of their characteristics over several passages. Using gingival fibroblasts from 3 dogs and 3 humans in the subculture (Sub), first (P1), third (P3), fifth (P5) and seventh (P7) passages, the following parameters were assessed: cell morphology, spreading, adhesion, viability and total protein content. The results showed no major differences between the passages in terms of morphology and spreading, and a tendency of greater adhesion and viability for HGF when compared with CGF. The total protein content was significantly higher for HGF. HGF exhibited greater functional and biochemical activity in vitro compared to CGF. Higher numbers at Sub were observed for both CGF and HGF in all evaluated parameters. The differences do not prevent the use of CGF for tissue engineering, but its use seems to be more appropriate in the subculture or first passage.


International Journal of Periodontics & Restorative Dentistry | 2016

Socket Preservation Therapy with Acellular Dermal Matrix and Mineralized Bone Allograft After Tooth Extraction in Humans: A Clinical and Histomorphometric Study.

Patrícia Garani Fernandes; Valdir Antonio Muglia; Danilo Maeda Reino; Luciana Prado Maia; de Moraes Grisi Mf; de Souza Sl; Mário Taba; Daniela B. Palioto; de Almeida Ag; Arthur B. Novaes

The aim of this study was to analyze through clinical and histomorphometric parameters the use of acellular dermal matrix (ADM) with or without mineralized bone allograft (AB) on bone formation in human alveoli after a 6- to 8-month healing period. A total of 19 patients in need of extraction of the maxillary anterior teeth were selected and randomly assigned to the test group (ADM plus AB) or to the control group (ADM only). Clinical and histomorphometric measurements and histologic analysis were recorded 6 to 8 months after ridge preservation procedures. Clinical parameters and amount of mineralized and nonmineralized tissue were measured and analyzed. In the clinical measurements, the test group showed reduced bone loss in the buccopalatal dimension after 6 to 8 months (intragroup analysis P < .01). Histologic findings showed higher percentages of mineralized tissue and lower percentages of nonmineralized tissue in the test group when compared with the control group (P < .05). In this randomized controlled clinical and histomorphometric study in humans, acellular dermal matrix in association with mineralized bone allograft reduced alveolar bone loss in the anterior maxillae both in height and width after a follow-up period of 6 to 8 months.


Clinical Oral Implants Research | 2015

The influence of the periodontal biotype on peri‐implant tissues around immediate implants with and without xenografts. Clinical and micro‐computerized tomographic study in small Beagle dogs

Luciana Prado Maia; Danilo Maeda Reino; Valdir Antonio Muglia; Sérgio Luís Scombatti de Souza; Daniela B. Palioto; Arthur B. Novaes

OBJECTIVE Soft tissues and buccal bone plate remodeling after immediate implantation in sockets with thin buccal bone, using the flapless approach with or without bone graft into the buccal gap, was compared between sites with thin and normal gingiva. MATERIAL AND METHODS Eight dogs had the gingiva of one side of the mandible thinned, the mandibular premolars were extracted without flaps, and 4 implants were installed in each side, positioned 1.5 mm from the buccal bone. The sites were randomly assigned into: TG (test group) = thin gingiva; TG + GM (TG with grafting material); CG (control group) = normal gingiva; and CG + GM (CG with grafting material). Buccal bone thickness (BBT), thickness of keratinized tissue (TKT), alveolar thickness (AT), gingival recession (GR), and probing depth (PD) were clinically evaluated. Within 12 weeks the dogs were sacrificed and the samples were analyzed by micro-computerized tomography. RESULTS A thin BBT was observed in all the dogs. The presurgical procedures reduced TKT in the test group, with minimal changes of the AT. There were no statistically significant differences among the groups for the clinical parameters and the tomographic analysis showed similar linear and tri-dimensional bone reduction in all the groups. CONCLUSION The thickness of the buccal bone was a fundamental factor in buccal bone plate resorption, even with flapless implantation. The decrease in gingival thickness or the addition of a biomaterial in the gap did not influence the results.

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Mário Taba

University of São Paulo

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