Luciana Reichert da Silva Assunção

Bone marrow mesenchymal stem cells stimulated by bFGF up-regulated protein expression in comparison with periodontal fibroblasts in vitro

OBJECTIVE The aim of this study was to evaluate, in vitro, the role of bFGF in the proliferation and expression of collagen type I and fibronectin of dog bone marrow mesenchymal stem cells (dBMMSCs) in comparison with the expression of the same proteins in dog periodontal fibroblasts (dPLFs). DESIGN dBMMSCs from the iliac crest were cultivated in Dulbeccos Modified Eagles Medium (DMEM). Flow cytometry analysis (FCA) was used to characterize dBMMSC. Cells were stimulated with bFGF (1, 5 and 10 ng/mL) after 24 and 48 h. Real time RT-PCR was performed to verify collagen type I and fibronectin expressions. MTT assay was used to confirm cellular proliferation. Statistical analyses were performed (ANOVA and Kruskal-Wallis tests; p<0.05). RESULTS FCA showed 55.98% of CD34+ and 32.67% of CD90+ after bone marrow aspiration; 3.33% of CD34+ and 33.0% of CD90+ before P1. After P2, 10.54% of dBMMSCs expressed CD90, whereas after P3, this number decreased to 1.58%. dPLFs presented 4.04% of CD90+ and 1.05% of CD34+ after P3. MTT evaluation showed increase in dBMSC proliferation with 5 ng/mL bFGF-stimulus after 24-h. Both collagen I and fibronectin expression were very similar between the two cells groups after 24-h stimulation with 1 ng/mL bFGF concentration. Fibronectin and collagen I expressions were higher after 24-h stimulation with 5 ng/mL bFGF. CONCLUSION dBMMSCs (1 ng/mL-bFGF stimulus after 24 h) are very similar to dPLFs as regards morphological and immunostaining characteristics, and collagen and/or fibronectin production. The dBMMSCs presented the highest protein expression rates with 5 ng/mL-bFGF stimulus after 24-h.

Association of parental attitudes at mealtime and snack limits with the prevalence of untreated dental caries among preschool children.

Eating behavior of parents exerts an influence on eating practices among their children, including the consumption of cariogenic products. The aim of this study was to determine the relationship between the snack limits established by parents/caregivers and the prevalence of untreated dental caries (UDC) among their children. A cross-sectional study was conducted with a representative sample of 686 children aged four and five years enrolled at public schools in the city of Curitiba, Brazil, and their parents/caregivers. The children were examined for dental caries and visible plaque. Parents/caregivers answered the Brazilian version of the Parent Mealtime Action Scale (PMAS). Data were analyzed using nonparametric tests and Poison regression analysis. The prevalence of UDC was 45.6%. The Snack Limits subscale of the PMAS was associated with a lower prevalence rate of UDC (PR: 0.83; 95%CI: 0.72-0.96), independently of the other variables. UDC was also associated with a greater prevalence of visible plaque (PR: 1.29; 95%CI: 1.08-1.54), a lower tooth brushing frequency (PR: 1.46; 95%CI: 1.22-1.77) and greater age of the child (PR: 1.31; 95%CI: 1.08-1.59). Snack limits established by parents/caregivers were associated to a lower prevalence rate of UDC among preschool children.

Terapia Endodôntica em Dentes Decíduos por Odontopediatras

Resumen pt: Objetivo: V erificar a conduta de odontopediatras quanto a escolha da terapia endodontica em dentes deciduos, assim como os fatores que influenciam essa ...

Emergency Dental Care of Children and Adolescents in Basic Health Unit and Emergency Care Unit

Objective: To evaluate the factors related to the dental emergency care of children and adolescents in Basic Health Units (UBS) and Emergency Care Units (UPA) affiliated with the public dental network of Curitiba, Brazil. Material and Methods: Cross-sectional study that evaluated 1,012 emergency cases in individuals aged up to 17 years old and with a permanent record in the Unified Health System of Curitiba. Demographic data of individuals and of services were taken from electronic medical records. Statistical analysis was performed using the chi-square test and chi-square test for linear trend (α = 0.05), estimating the prevalence ratio (PR) (95%CI). Results: Of the total of emergency cases, 68.2% and 31.8% were of children and adolescents, respectively. UBS accounted for 89.7% of the cases while UPA represented 10.3%, and 12.2% of the treatments were characterized as first access to the system. Adolescents used UPAs for emergency care more often than children (PR = 1.84; 95%CI: 1.28-2.64). Among children, those with more than 1 year since the last appointment used UPAs for emergency care more often (PR = 1.86; 95%CI: 1.09-3.17). Adolescents for whom emergency care was characterized as first access to the system used UPAs more often (PR = 2.16; 95%CI: 1.04-4.46). The proximity between the UBS of origin and the UPA was associated with greater UPA utilization by both groups. Conclusion: UBSs accounted for most of the dental emergency treatments. Children and adolescents with a poorer connection with UBSs and those who lived near an UPA had a higher prevalence of UPA utilization for emergency care.

Oral Findings in Brazilian Infants Born at Full Term

Objective: To evaluate the oral findings of Brazilian infants born at full term as well as to identify the presence of abnormalities at birth. Material and Methods: Cross-sectional study comprising 190 infants aged up to 2 days old. Data were obtained by applying a structured questionnaire to the mothers as well as by collecting information from the infants’ medical records. Clinical examination was conducted to evaluate the infants’ oral features and it included the relationship of alveolar ridges and shape of the gum pads and the insertion of the lingual and labial frenula. The presence of oral developmental abnormalities was also evaluated. Chi-square and Mann-Whitney U tests were used to analyze the infants’ abnormalities and gender (α = 0.05). Results: Most infants presented round upper gum pads (71.6%) and U-shaped lower one (52.6%). The mean discrepancy between the alveolar ridges was 3.33mm. Interference with tongue movement was found in 12.6% of the newborns. There was 50.0% prevalence of oral cysts. Among them, Epstein pearl was found in 27.4% of the cases, Bohn nodules in 26.3%, and dental lamina cysts in 11.1%. There was no significant difference between the occurrence of abnormalities and the infants’ gender. The normal features of the oral cavity meet the literature data. The herein found congenital developmental abnormalities were oral cysts and ankyloglossia. Conclusion: Different classification forms made it difficult to compare the current study data with those from other studies. Therefore, it suggests the need for standardization in order to evaluate these abnormalities.