Helder Henrique Costa Pinheiro

High prevalence of G12P[8] rotavirus strains in Rio Branco, Acre, Western Amazon, in the post‐rotavirus vaccine introduction period

The present study aimed to provide a molecular characterization of circulating rotavirus (RVA) strains in Rio Branco, Acre, in the post‐rotavirus vaccination period, particularly with regard to the emerging, increasingly prevalent G12P[8] genotype. A total of 488 fecal specimens from diarrheic and non‐diarrheic children were obtained between January and December 2012. RVA detection was initially performed using enzyme‐linked immunosorbent assay (ELISA) method, followed by reverse‐transcription polymerase chain reaction (RT‐PCR) using specific primers. RVA was detected in 18.3% (44/241) of the children with acute diarrhea and in 1.2% (3/247) of the non‐diarrheic children (Pu2009<u20090.001), with overall RVA‐positivity of 9.6% (47/488). The most common genotype was G2P[4] with 43.2% (19/44) of the diarrheic cases, followed by G12P[8] (27.3%, 12/44), G3P[6] (18.2%, 8/44), G3P[8] (4.5%, 2/44), and G12P[6] (2.3%, 1/44). G12 samples belonged to lineage III and were from children aged 4–52 months. All of these children had acute diarrhea associated with fever (83.3%, 10/12) and vomiting (66.7%, 8/12). Most of the cases occurred in August (58.3%, 7/12), 75% (9/12) of which having received the full vaccination scheme with Rotarix™. For the first time G12 was reported at relative high prevalence in Brazil. Our findings warrant further monitoring studies on the molecular characterization of circulating RVA strains after rotavirus vaccine introduction in Brazil and elsewhere, since the occurrence of either unusual our emerging genotypes may pose a challenge to vaccination strategies. J. Med. Virol. 88:782–789, 2016.

Terapia Endodôntica em Dentes Decíduos por Odontopediatras

Resumen pt: Objetivo: V erificar a conduta de odontopediatras quanto a escolha da terapia endodontica em dentes deciduos, assim como os fatores que influenciam essa ...

Prevalence of human papillomavirus (HPV) in Belém, Pará State, Brazil, in the oral cavity of individuals without clinically diagnosable injuries

This cross-sectional study aimed to determine HPV prevalence in the oral cavity of individuals without clinically diagnosable lesions and to identify the respective HPV types. A total of 166 samples were analyzed from patients 18 years or older in the State of Pará, Brazil. Samples were collected by sterile brush scraping in the oral cavity. HPV detection used polymerase chain reaction (PCR). Infected samples were typed as HPV 6, 11, 16, 18, 31, 33, 35, 52, and 58. HPV was present in 40 samples (24.1%). Three samples (7.5%) were positive for HPV 6, five (12.5%) for HPV 18, and one (2.5%) for HPV 58.Trata-se de um estudo transversal com o objetivo de verificar a prevalencia do HPV na cavidade oral de individuos sem lesoes clinicamente diagnosticaveis e quais sao os tipos encontrados neles. Foram analisadas 166 amostras em pacientes maiores de 18 anos de idade, residentes no Estado do Para, Brasil. As amostras foram coletadas por meio de raspado com escova esteril na cavidade oral. Para a deteccao da presenca do HPV, foi utilizada a tecnica da reacao em cadeia da polimerase (PCR). As amostras infectadas pelo HPV foram tipadas para HPV 6, 11, 16, 18, 31, 33, 35, 52 e 58. Os resultados encontrados indicaram a presenca de HPV em 40 amostras (24,1%). Tres amostras (7,5%) foram positivas para HPV 6; cinco (12,5%), para HPV 18; e uma (2,5%), para HPV 58.

Prevalência do papilomavírus humano (HPV) em Belém, Pará, Brasil, na cavidade oral de indivíduos sem lesões clinicamente diagnosticáveis

This cross-sectional study aimed to determine HPV prevalence in the oral cavity of individuals without clinically diagnosable lesions and to identify the respective HPV types. A total of 166 samples were analyzed from patients 18 years or older in the State of Pará, Brazil. Samples were collected by sterile brush scraping in the oral cavity. HPV detection used polymerase chain reaction (PCR). Infected samples were typed as HPV 6, 11, 16, 18, 31, 33, 35, 52, and 58. HPV was present in 40 samples (24.1%). Three samples (7.5%) were positive for HPV 6, five (12.5%) for HPV 18, and one (2.5%) for HPV 58.Trata-se de um estudo transversal com o objetivo de verificar a prevalencia do HPV na cavidade oral de individuos sem lesoes clinicamente diagnosticaveis e quais sao os tipos encontrados neles. Foram analisadas 166 amostras em pacientes maiores de 18 anos de idade, residentes no Estado do Para, Brasil. As amostras foram coletadas por meio de raspado com escova esteril na cavidade oral. Para a deteccao da presenca do HPV, foi utilizada a tecnica da reacao em cadeia da polimerase (PCR). As amostras infectadas pelo HPV foram tipadas para HPV 6, 11, 16, 18, 31, 33, 35, 52 e 58. Os resultados encontrados indicaram a presenca de HPV em 40 amostras (24,1%). Tres amostras (7,5%) foram positivas para HPV 6; cinco (12,5%), para HPV 18; e uma (2,5%), para HPV 58.

O modelo de vigilância da água e a divulgação de indicadores de concentração de fluoreto

Water surveillance is an essential strategy to ensure safety and high quality standards for human consumption. Among the parameters, fluoride stands out. The objective of this study was to describe the surveillance framework of water quality and present a proposal of indicators and dissemination of information to the population regarding the fluoride concentration indicators. Based on main scientific evidences, it is explained the importance of maintaining the parameter under control in the supplies systems and it is presented a concise description of the water surveillance framework in Brazil, showing that the design does not offer indicators for longitudinally monitoring the parameter or visibility to all those who have right to information. Next, the operational and technical foundations of Public Water Supply Fluoridation Surveillance System are presented, a proposal to improve the framework. Indicators to the dissemination of the information are described, arguing that the Country has resources and conditions to overcome the precariousness of information on fluoride concentration and on the coverage of water fluoridation in all units of national territory.

La prevalencia del virus del papiloma humano (VPH) en Belém, Pará, Brasil, en la cavidad oral de los individuos sin lesiones clínicamente diagnosticables

This cross-sectional study aimed to determine HPV prevalence in the oral cavity of individuals without clinically diagnosable lesions and to identify the respective HPV types. A total of 166 samples were analyzed from patients 18 years or older in the State of Pará, Brazil. Samples were collected by sterile brush scraping in the oral cavity. HPV detection used polymerase chain reaction (PCR). Infected samples were typed as HPV 6, 11, 16, 18, 31, 33, 35, 52, and 58. HPV was present in 40 samples (24.1%). Three samples (7.5%) were positive for HPV 6, five (12.5%) for HPV 18, and one (2.5%) for HPV 58.Trata-se de um estudo transversal com o objetivo de verificar a prevalencia do HPV na cavidade oral de individuos sem lesoes clinicamente diagnosticaveis e quais sao os tipos encontrados neles. Foram analisadas 166 amostras em pacientes maiores de 18 anos de idade, residentes no Estado do Para, Brasil. As amostras foram coletadas por meio de raspado com escova esteril na cavidade oral. Para a deteccao da presenca do HPV, foi utilizada a tecnica da reacao em cadeia da polimerase (PCR). As amostras infectadas pelo HPV foram tipadas para HPV 6, 11, 16, 18, 31, 33, 35, 52 e 58. Os resultados encontrados indicaram a presenca de HPV em 40 amostras (24,1%). Tres amostras (7,5%) foram positivas para HPV 6; cinco (12,5%), para HPV 18; e uma (2,5%), para HPV 58.

Perfil dos pacientes com necessidades especiais de uma clínica de odontopediatria - doi:10.5020/18061230.2011.p136

Objective: To assess the characteristics of special needs patients assisted at the Clinic of Pediatric Dentistry, Faculty of Dentistry, Federal University of Para. Methods: A descriptive nobservational study conducted from March 2007 to December 2009, assessing 137 records of which were extracted the following data: gender, age, origin, current and past medical nhistory, type of special needs and major oral diseases. We applied descriptive statistics, one-dimensional frequency table and prepared graphs of the results. Results: We found 77 n(56.2%) male patients, with mean age of 8.1 years; 118 (86.2%) living in the state capital of Para, 21 (15.3%) with respiratory problems, 37 (27.3%) with some allergy to medications and 54 (39.4%) making use of controlled drugs. Regarding the type of special need, we observed that 29 (28.3%) had neurologic and psychomotor delay and the primary oral disease found was dental caries, affecting 84 (61.3%) subjects. Conclusion: The assessed profile of patients with special needs is characterized by patients residing in the state capital of Para, with predominance of male children, with neurologic and psychomotor delay, with a mean age of 8.1 years, presenting mainly caries