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Dive into the research topics where Luciano Takeshi Kishi is active.

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Featured researches published by Luciano Takeshi Kishi.


Revista Brasileira De Fruticultura | 2008

Evaluation of Xylella fastidiosa genetic diversity by fAFPL markers

Luciano Takeshi Kishi; Ester Wickert; Eliana Gertrudes de Macedo Lemos

The first phytopathogenic bacterium with its DNA entirely sequenced is being detected and isolated from different host plants in several geographic regions. Although it causes diseases in cultures of economic importance, such as citrus, coffee, and grapevine little is known about the genetic relationships among different strains. Actually, all strains are grouped as a single species, Xylella fastidiosa, despite colonizing different hosts, developing symptoms, and different physiological and microbiological observed conditions. The existence of genetic diversity among X. fastidiosa strains was detected by different methodological techniques, since cultural to molecular methods. However, little is know about the phylogenetic relationships developed by Brazilian strains obtained from coffee and citrus plants. In order to evaluate it, fAFLP markers were used to verify genetic diversity and phylogenetic relationships developed by Brazilian and strange strains. fAFLP is an efficient technique, with high reproducibility that is currently used for bacterial typing and classification. The obtained results showed that Brazilian strains present genetic diversity and that the strains from this study were grouped distinctly according host and geographical origin like citrus-coffee, temecula-grapevine-mulberry and plum-elm.


The Scientific World Journal | 2012

Genetic Diversity and Population Differentiation of Guignardia mangiferae from “Tahiti” Acid Lime

Ester Wickert; Eliana Gertrudes de Macedo Lemos; Luciano Takeshi Kishi; Andressa de Souza; Antonio de Goes

Among the citrus plants, “Tahiti” acid lime is known as a host of G. mangiferae fungi. This species is considered endophytic for citrus plants and is easily isolated from asymptomatic fruits and leaves. G. mangiferae is genetically related and sometimes confused with G. citricarpa which causes Citrus Black Spot (CBS). “Tahiti” acid lime is one of the few species that means to be resistant to this disease because it does not present symptoms. Despite the fact that it is commonly found in citric plants, little is known about the populations of G. mangiferae associated with these plants. Hence, the objective of this work was to gain insights about the genetic diversity of the G. mangiferae populations that colonize “Tahiti” acid limes by sequencing cistron ITS1-5.8S-ITS2. It was verified that “Tahiti” acid lime plants are hosts of G. mangiferae and also of G. citricarpa, without presenting symptoms of CBS. Populations of G. mangiferae present low-to-moderate genetic diversity and show little-to-moderate levels of population differentiation. As gene flow was detected among the studied populations and they share haplotypes, it is possible that all populations, from citrus plants and also from the other known hosts of this fungus, belong to one great panmictic population.


BMC Microbiology | 2016

Reclassification of the taxonomic status of SEMIA3007 isolated in Mexico B-11A Mex as Rhizobium leguminosarum bv. viceae by bioinformatic tools

Luciano Takeshi Kishi; Camila C. Fernandes; Wellington Pine Omori; João Carlos Campanharo; Eliana Gertrudes de Macedo Lemos

BackgroundEvidence based on genomic sequences is extremely important to confirm the phylogenetic relationships within the Rhizobium group. SEMIA3007 was analyzed within the Mesorhizobium groups to define the underlying causes of taxonomic identification. We previously used biochemical tests and phenotypic taxonomic methods to identify bacteria, which can lead to erroneous classification. An improved understanding of bacterial strains such as the Mesorhizobium genus would increase our knowledge of classification and evolution of these species.ResultsIn this study, we sequenced the complete genome of SEMIA3007 and compared it with five other Mesorhizobium and two Rhizobium genomes. The genomes of isolated SEMIA3007 showed several orthologs with M. huakuii, M. erdmanii and M. loti. We identified SEMIA3007 as a Mesorhizobium by comparing the 16S rRNA gene and the complete genome.ConclusionOur ortholog, 16S rRNA gene and average nucleotide identity values (ANI) analysis all demonstrate SEMIA3007 is not Rhizobium leguminosarum bv. viceae. The results of the phylogenetic analysis clearly show SEMIA3007 is part of the Mesorhizobium group and suggest a reclassification is warranted.


Brazilian Journal of Microbiology | 2018

Bacterial communities in mining soils and surrounding areas under regeneration process in a former ore mine

Camila C. Fernandes; Luciano Takeshi Kishi; Erica Mendes Lopes; Wellington Pine Omori; Jackson Antônio Marcondes de Souza; Lucia Maria Carareto Alves; Eliana Gertrudes de Macedo Lemos

Human activities on the Earths surface change the landscape of natural ecosystems. Mining practices are one of the most severe human activities, drastically altering the chemical, physical and biological properties of the soil environment. Bacterial communities in soil play an important role in the maintenance of ecological relationships. This work shows bacterial diversity, metabolic repertoire and physiological behavior in five ecosystems samples with different levels of impact. These ecosystems belong to a historical area in Iron Quadrangle, Minas Gerais, Brazil, which suffered mining activities until its total depletion without recovery since today. The results revealed Proteobacteria as the most predominant phylum followed by Acidobacteria, Verrucomicrobia, Planctomycetes, and Bacteroidetes. Soils that have not undergone anthropological actions exhibit an increase ability to degrade carbon sources. The richest soil with the high diversity was found in ecosystems that have suffered anthropogenic action. Our study shows profile of diversity inferring metabolic profile, which may elucidate the mechanisms underlying changes in community structure in situ mining sites in Brazil. Our data comes from contributing to know the bacterial diversity, relationship between these bacteria and can explore strategies for natural bioremediation in mining areas or adjacent areas under regeneration process in iron mining areas.


Archive | 2017

Soil Microbiome and Their Effects on Nutrient Management for Plants

Rosangela Naomi Inui Kishi; Renato Fernandes Galdiano Júnior; Silvana Pompéia Val-Moraes; Luciano Takeshi Kishi

The soil microbiome is a diverse system composed of microorganisms with different functions. Microorganisms known as plant growth-promoting microorganisms (PGPMs) can help plants with nutrient uptake and consequently with crop yields. From this class of microorganisms, we can isolate nitrogen-fixing bacteria (NFB), phosphorus-solubilizing microorganisms (PSMs), and the microbes that are able to produce phytohormones. The use of these microorganisms in improving nutrient uptake by plants has been acceptable because of reduced costs and the safety of application for humans and the environment. It is for this reason that inoculant products have been developed. During the process of inoculant development, it is possible to use molecular biology techniques, such as 16S rRNA gene sequencing. This technique helps with the identification of potential microorganisms adapted for different conditions and crops. Moreover, these microorganisms can be used in degradable areas or as pathogen controls. It is also important to consider the siderophore, which is a biological molecule produced by various bacteria, and which has an immense application in agriculture. Another important symbiosis that occurs is realized by mycorrhizas, which are essential for transferring nutrients and water from the soil to plants.


Genome Announcements | 2017

Draft Genome Sequence of Bradyrhizobium elkanii TnphoA 33, a Producer of Polyhydroxyalkanoates

Erica Mendes Lopes; Luciano Takeshi Kishi; Camila C. Fernandes; Fernanda Larozza Paganelli; Lucia Maria Carareto Alves; Eliana Gertrudes de Macedo Lemos; Jackson Antônio Marcondes de Souza

ABSTRACT The genus Bradyrhizobium comprises bacteria with the ability to form nitrogen-fixing symbioses with legumes. They are of great interest in agriculture, as well as for the production of biopolymers such as polyhydroxyalkanoates. Here, we report the draft genome assembly of Bradyrhizobium elkanii TnphoA 33 comprising 9 Mb, 1,124 contigs, and 9,418 open reading frames.


Genome Announcements | 2017

Draft Genome Sequence of a Chitinophaga Strain Isolated from a Lignocellulose Biomass-Degrading Consortium.

Luciano Takeshi Kishi; Erica Mendes Lopes; Camila C. Fernandes; Gabriela Cabral Fernandes; Laís Postai Sacco; Lucia Maria Carareto Alves; Eliana Gertrudes de Macedo Lemos

ABSTRACT Chitinophaga comprises microorganisms capable of degrading plant-derived carbohydrates, serving as a source of new tools for the characterization and degradation of plant biomass. Here, we report the draft genome assembly of a Chitinophaga strain with 8.2 Mbp and 7,173 open reading frames (ORFs), isolated from a bacterial consortium that is able to degrade lignocellulose.


The Scientific World Journal | 2012

Erratum to “Genetic Diversity and Population Differentiation of Guignardia mangiferae from “Tahiti” Acid Lime”

Ester Wickert; Eliana Gertrudes de Macedo Lemos; Luciano Takeshi Kishi; Andressa de Souza; Antonio de Goes


ARPN journal of engineering and applied sciences | 2012

PHOSPHORUS SOLUBILIZING AND IAA PRODUCTION ACTIVITIES IN PLANT GROWTH PROMOTING RHIZOBACTERIA FROM BRAZILIAN SOILS UNDER SUGARCANE CULTIVATION

Rosangela Naomi Inui-Kishi; Luciano Takeshi Kishi; Simone Cristina Picchi; José Carlos Barbosa; Maria Teresa Olivério Lemos; Jackson Marcondes; Eliana Gertrudes de Macedo Lemos


Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology | 2016

Liming in the sugarcane burnt system and the green harvest practice affect soil bacterial community in northeastern São Paulo, Brazil

Silvana Pompéia Val-Moraes; Helena Suleiman de Macedo; Luciano Takeshi Kishi; Rodrigo Matheus Pereira; Acacio Aparecido Navarrete; Lucas William Mendes; Eduardo Barretto de Figueiredo; Newton La Scala; Siu Mui Tsai; Eliana Gertrudes de Macedo Lemos; Lucia Maria Carareto Alves

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Ester Wickert

Universidade Federal do Rio Grande do Sul

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Rodrigo Matheus Pereira

Universidade Federal da Grande Dourados

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Siu Mui Tsai

University of São Paulo

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