Luisa Massaccesi

Long-term distribution, mobility and plant availability of compost-derived heavy metals in a landfill covering soil

The application of municipal waste compost (MWC) and other organic materials may serve to enhance soil fertility of earthen materials and mine spoils used in land reclamation activities, particularly in the recovery of degraded areas left by exhausted quarries, mines and landfill sites among others. The long-term distribution, mobility and phytoavailability of heavy metals in such anthropogenic soils were studied by collecting soil samples at different depths over a 10 y chronosequence subsequent to amendment of the top layer of a landfill covering soil with a single dose of mechanically-separated MWC. Amendment resulted in a significant enhancement of the metal loadings in the amended topsoils particularly for Cu, Zn and Pb, which were also the predominant metals in the compost utilised. Although metals were predominantly retained in the compost amended soil horizon, with time their vertical distribution resulted in a moderate enrichment of the underlying mineral horizons, not directly influenced by compost amendment. This enrichment generally resulted from the leaching of soluble organo-metal complexes and subsequent adsorption to mineral horizons. However, in the course of the 10-y experimental period, metal concentrations in the underlying horizons generally returned to background concentrations suggesting a potential loss of metals from the soil system. Analysis of the tissues of plants growing spontaneously on the landfill site suggests that metal phytoavailability was limited and generally species-dependent.

Co-treatment of fruit and vegetable waste in sludge digesters. An analysis of the relationship among bio-methane generation, process stability and digestate phytotoxicity.

The co-digestion of a variable amount of fruit and vegetable waste in a waste mixed sludge digester was investigated using a pilot scale apparatus. The organic loading rate (OLR) was increased from 1.46 kg VS/m(3) day to 2.8 kg VS/m(3) day. The hydraulic retention time was reduced from 14 days to about 10 days. Specific bio-methane production increased from about 90 NL/kg VS to the maximum value of about 430 NL/kg VS when OLR was increased from 1.46 kg VS/m(3) day to 2.1 kg VS/m(3) day. A higher OLR caused an excessive reduction in the hydraulic retention time, enhancing microorganism wash out. Process stability evaluated by the total volatile fatty acids concentration (mg/l) to the alkalinity buffer capacity (eq. mg/l CaCO3) ratio (i.e. FOS/TAC) criterion was <0.1 indicating high stability for OLR <2.46 kg VS/m(3 )day. For higher OLR, FOS/TAC increased rapidly. Residual phytotoxicty of the digestate evaluated by the germination index (GI) (%) was quite constant for OLR<2.46 kg VS/m(3)day, which is lower than the 60% limit, indicating an acceptable toxicity level for crops. For OLR>2.46 kg VS/m(3) day, GI decreased rapidly. This corresponding trend between FOS/TAC and GI was further investigated by the definition of the GI ratio (GIR) parameter. Comparison between GIR and FOS/TAC suggests that GI could be a suitable criterion for evaluating process stability.

Hybrid solid anaerobic digestion batch: biomethane production and mass recovery from the organic fraction of solid waste

An experimental apparatus was constructed to perform hybrid solid anaerobic digestion batch processing of the organic fraction of municipal solid waste. The preliminary process was carried out with a high total solids concentration of about 33% w w−1 and with an initial organic load of about 340 kg VS kg−1. The fresh organic fraction to inoculum ratio used to enhance the anaerobic process start-up was 0.910 kg VS kg VS−1. The process was conducted by spreading the percolate on top of the mixture. The percolate was stored in a separate section of the apparatus with a mean hydraulic retention time of about 1 day. During the process, acetate, butyrate and propionate in the percolate reached concentrations ranging from 3000 to 11 000 mg L−1. In spite of these high concentrations, the biomethane produced from both the solid and the percolate was quite high, at about 210 NL kg VS−1. The digestate obtained at the end of the run showed rather good features for being classified as an organic fertilizer according to Italian law. However, a residual phytotoxicity level was detected by a standardized test showing a germination index of about 50%.

Holm oak (Quercus ilex L.) rhizosphere affects limestone-derived soil under a multi-centennial forest

Background and AimsThe activity of roots and associated microorganisms plays a key-role in soil formation and evolution, but we lack of knowledge on the quality and extent of the “rhizosphere effect” in the different soil horizons. The aim of this study was to assess the interactions between rhizosphere processes and genetic horizons in a forest (Quercus ilex L.) soil developed from limestone. Specifically, we tested (a) if the rhizosphere effect was significant in all the horizons of the soil profiles, and (b) if the intensity of the rhizosphere effect was associated to structure, composition and activity of the microbial community.MethodsBulk and rhizosphere soils were characterized by physical, mineralogical, chemical and biological (microbial activity and community structure) analyses.ResultsThroughout the soil profile, the rhizosphere processes affected properties like particle-size distribution and soil structure, mineralogy, pH, and organic C and total P content. Conversely, amounts of exchangeable Ca, Mg and K, iron oxides, available P, and total nitrogen showed no significant change. As for the microbial community, its structure and metabolic activity differed between rhizosphere and bulk only in the core of the solum (2Bwb and 3Bwb horizons).ConclusionsThe main processes controlling the intensity of the rhizosphere effect on the soil horizons were root activity, soil faunal perturbation and slope dynamics. While root activity impacted the whole soil profile, although to a lesser extent at depth, the influence of fauna and slope was confined atop the profile. It follows that long-term changes due to root activity and associated microbial community were more strongly expressed in the core of the solum, not at the surface, of this limestone-derived soil.

Long Term Amendment with Fresh and Composted Solid Olive Mill Waste on Olive Grove Affects Carbon Sequestration by Prunings, Fruits, and Soil

The soil amendment with organic wastes represents a way to increase the soil fertility and the organic carbon (C) stored in the agro-ecosystems. Among the organic waste materials produced by agricultural and industrial activities, olive mill wastes derived from the olive oil extraction process may represent a suitable soil amendment. The aim of the study was to evaluate the effect of fresh (SOMW) or composted mixture of SOMW and shredded olive tree prunings (C-SOMW+P) on the vegetative and productive activities of olive trees, on the C stored in the tree non-permanent structures (prunings and fruits) and in the soil. The plots treated with SOMW or C-SOMW+P showed higher vegetative and productive activities than the untreated plots, and this was attributed to the higher total N and availability of P and K supplied by the amendments. Consequently, treatments increased the C sequestered in the tree non-permanent structures than in the control trees. However, no significant different effect between SOMW and C-SOMW+P treatments was found for the C stored in prunings and fruits, whereas it was evident a stronger influence of C-SOMW+P than SOMW on soil C sequestration. Indeed, about 50% the C supplied by the treatment with C-SOMW+P was sequestered in the olive grove system, with more than 90% of the sequestered C stored into the soil. The low amount of C sequestered in the soil following the addition of SOMW was attributed to its richness of moisture and easily degradable compounds that triggered the mineralization processes controlled by the soil microbial community. Although the 8 years of amendment produced a higher fruit yields than the control, no difference occurred between the characteristics and the oil content of the olive fruits. Only the total phenol content for the oil obtained from the SOMW-treated plots was significantly higher. The other considered fruit characteristics did not show significant differences.

Co-treatment of fruit and vegetable waste in sludge digesters: Chemical and spectroscopic investigation by fluorescence and Fourier transform infrared spectroscopy.

In a previous work co-digestion of food waste and sewage sludge was performed in a pilot apparatus reproducing operating conditions of an existing full scale digester and processing waste mixed sludge (WMS) and fruit and vegetable waste (FVW) at different organic loading rates. An analysis of the relationship among bio-methane generation, process stability and digestate phytotoxicity was conducted. In this paper we considered humification parameters and spectroscopic analysis. Humification parameters indicated a higher not humified fraction (NH) and a lower degree of humification (DH) of FVW with respect to WMS (NH=19.22 and 5.10%; DH=36.65 and 61.94% for FVW and WMS, respectively) associated with their different chemical compositions and with the stabilization process previously undergone by sludge. FVW additions seemed to be favourable from an agronomical point of view since a lower percentage of organic carbon was lost. Fourier transform infrared spectra suggested consumption of aliphatics associated with rising in bio-methane generation followed by accumulation of aliphatics and carboxylic acids when the biogas production dropped. The trend of peaks ratios can be used as an indicator of the process efficiency. Fluorescence intensity of peak B associated with tryptophan-like substances and peak D associated with humic-like substances observed on tridimensional Excitation Emission Matrix maps increased up to sample corresponding to the highest rate of biogas production. Overall spectroscopic results provided evidence of different chemical pathways of anaerobic digestion associated with increasing amount of FVW which led to different levels of biogas production.

Factors Involved in Uptake of Lead by Some Edible Crops Grown in Agricultural Soils of Central Italy

This study was organized to investigate the bioavailability of Pb in soil, as affected by soil type and crop species. The four most representative forms of Pb in soil (exchangeable, bound to carbonates, bound to the Fe-Mn oxides, and bound to organic matter) were considered, and their respective contribution to the uptake of this metal was determined. Four edible crops (lettuce, radish, tomato, and Italian ryegrass) were grown in four soil types, both nonspiked and spiked with 200 mg Pb kg−1. Chemical analyses were carried out to determine the amount of Pb sorbed from the soil and stored in the different plant organs. The bioaccumulation factor (BAF) and the translocation factor were derived and used to compare plant species. Results showed that all the test plants sorbed 10 to 30 times more Pb from spiked soils, as compared with nonspiked soils. The BAF and translocation factor showed that, excluding Italian ryegrass, all the crops sorbed less Pb from the soils containing Ca carbonate. On the basis of BAF, lettuce, tomato, and Italian ryegrass could be classified as crops with high Pb uptake ability in all soils. Concerning the distribution of Pb within the plant, all species showed the tendency to store this metal mainly in roots. The translocation to above-ground organs was the highest with lettuce, tomato (with reference to non edible above-ground organs) and Italian ryegrass, whereas radish showed the lowest translocation ability, together with the lowest uptake ability.

Multi-approach characterization of organic sediment produced by an anaerobic digestion plant fed with pig slurry and stored for a long term in a lagoon

This study combined different approaches to characterize organic sediments produced by an anaerobic digestion plant feed with pig slurry, and accumulated for many years in a lagoon. The results of all analyses identified a certain homogeneity of the sediments. As a consequence of the pig diet, the sediment contained an high concentration of Zn (about 4gkg-1) and Cu (about 1.2gkg-1), which were mostly associated to the particles with a size ranging from 2 to 53μm. The sediment was made of large amount of organic matter, mostly cellulose and recalcitrant molecules, and 30-40% mineral fraction. XANES and XES spectroscopies indicated the presence of zinc phosphate (38%), zinc sulfide (32%), zinc carbonate (19%), and zinc oxide (11%). The presence in the sediment of forms characterized by a very scarce solubility, as also confirmed by the Zn and Cu chemical speciation, indicated a low bioavailability of these metals. However, although their low mobility, the high concentrations of Zn and Cu allowed to consider the sediment not suitable to use as a fertiliser due to the potential risk of metal interaction with the food chain.

Organic carbon pools and storage in the soil of olive groves of different age: Soil organic carbon in olive groves

SUMMARY: Compared with annual crop cultivation, tree groves might represent a relevant land‐use system to improve C sequestration, but few data are available to support this hypothesis. To evaluate the potential of olive tree (Olea europaea L., 1753) cultivation to store soil organic C (SOC), we assessed (i) the distribution of organic C in active (water‐extractable and particulate organic C, WEOC and POC, respectively), intermediate (organic matter associated with stable sand‐size aggregates and silt‐ and clay‐size aggregates, SSAs and SCAs, respectively) and passive (organic matter resistant to oxidation, rSOM) pools, (ii) the phenol content of the C pools, (iii) the humic‐C distribution of the intermediate C pool and (iv) the stocks of SOC pools in two olive groves of different age (7 years (OG7) and 30 years (OG30)) compared with a nearby site with cereal crops (arable soil, AS). In OG30 the organic C stock of the olive grove was no different from that of the AS, but the distribution of SOC pools changed with the age of the olive groves. The WEOC and POC increased in the Ap horizon of OGs, probably because of the herbaceous cover and distribution of chipped prunings on the soil. There were fewer SSAs in OG7 than AS, possibly because of pedoturbations from deep tillage before the olive trees were established, but they increased in OG30. The increase in SSAs and SCAs in the Bw and BC horizons of OG30 was associated with humic‐C and unextractable‐C and a smaller phenol content than AS. This suggested that the olive tree roots had a positive role through rhizodeposition and root turnover, which favoured the stabilization of organic matter into aggregates at depth. In contrast to the active and intermediate C pools, the passive C pool did not vary following the change in land use from arable to olive grove. HIGHLIGHTS: Effects of land‐use change from arable to olive grove on soil organic C pools and stocks. Soil organic C stock increased from 7‐ to 30‐year‐old olive orchard. Olive tree cultivation affected active and intermediate C pools, but not the passive C pool. After 30 years, the olive grove stored an amount of SOC similar to that of the arable system.