Luiz Augusto de Souza

Tratamento de hérnia umbilical em bovinos

Bovinos jovens sao frequentemente acometidos por hernias umbilicais. Neste trabalho, avaliou-se a ocorrencia e estabeleceu-se o tratamento cirurgico para hernias umbilicais em bovinos jovens, empregando 78 bezerros distribuidos em seis grupos. Em GI e GIV, na laparorrafia, utilizou-se sutura jaquetao; em GII e GV, jaquetao modificado e, em GIII e GVI, sutura separada simples com pontos de relaxamento. Em GI, GII e GIII utilizou-se fio de algodao e, em GIV, GV e GVI, de nailon. Dados nao parametricos, envolvendo a recuperacao dos animais e sua independencia ou associacoes com o padrao de sutura foram analisados pelo teste do Qui-quadrado e Exato de Fisher, fazendo-se uso do programa computacional Statistical Analysis System, a 5% (p < 0,05). Os demais resultados foram analisados descritivamente. A ocorrencia de hernia umbilical em bovinos foi expressiva e a herniorrafia, empregando-se fio de algodao, apresentou maiores complicacoes pos-operatorias; porem a maioria dos animais recuperou-se. O fio e o padrao de sutura, manejo pos-operatorio, resposta individual e o tamanho do anel herniario influenciam na reabilitacao dos animais e na reducao das complicacoes pos-operatorias.

Método imunomagnético associado ao meio MesenCult® na obtenção de células mononucleares da medula óssea de coelhos negativas para o anticorpo monoclonal CD45

The objective of this study was to describe guidelines for the isolation of bone marrow mononuclear cells from rabbits, followed by cell purification by negative depletion with CD45 monoclonal antibody, and further expansion in MesenCult® medium. Ten adult male New Zealand White rabbits, age average of 1.0±0.2 years and weighting 3.5±0.24kg, were used to obtain a standardized method. The mononuclear cells of the bone marrow were isolated with Ficoll-paque® density gradient centrifugation, and the cell purification and acquisition was completed by negative depletion with CD45 monoclonal antibody in immunomagnetic base. The cell population obtained was expanded in MesenCult® medium. Through isolation with Ficoll-paque® density gradient was possible to obtain an average yield of 7.31x106 cells/mL. After purification and acquisiton of potential mesenchymal stem cells by the immunomagnetic base, there was a yield decrease to 2.28x106 cells/mL; however the expansion process was increased in cell culture. The results indicated that cells obtained from the mononuclear fraction of bone marrow and cultivated in vitro were capable to generate adherent cells 24 hours after culture, with predominance of fibroblastoid cells suggestive of mesenchymal stem cells. It can be concluded that mesenchymal stem cells can be achieved with purified rabbit bone marrow mononuclear cells through the immunomagnetic method, as the MesenCult® medium provides a suitable environment for a quick in vitro expansion, and the number of passages exerts negative influence on the morphological characteristics.