Luiz Eduardo Dias

Availability of phosphorus in a Brazilian Oxisol cultivated with eucalyptus after nine years as influenced by phosphorus‐fertilizer source, rate, and placement

Abstract This work evaluated the effect of different placement and rates of two phosphorus (P) fertilizers on P‐availability by three methods of extraction, nine years after application to a Brazilian Oxisol cultivated with Eucalyptus camaldulensis. The treatments were applied to 24x18 m plots and 96 seedlings of E. camaldulensis were planted (3.0x1.5 m) in each plot. Single superphosphate (SSP) and rock phosphate (RP) were tested in three rates (100, 200, and 400 kg ha‐1 of P2O5). Each fertilizer was either (1) surface‐applied in bands (0.6 m either side of the rows of trees) and incorporated before planting or (2) incorporated into furrows (0.2 m deep in the tree rows) before planting. As additional treatments, the combination of RP (96 kg ha‐1 of P2O5 applied in broadcast, or bands, or in furrows) + SSP (54 kg ha‐1 of P2O5 localized in the planting hole before planting) were tested. Twelve soil subsamples from two layers (0–15 and 25–40 cm) were taken from each plot (from the planting rows or between the planting rows) and were analyzed for pH in water (1:2.5), available P by Mehlich‐1, Bray‐1 and anionic resin, exchangeable Ca, and Al by 1 mol L‐1 Kcl. For both methods of fertilizers placement, the highest values of available P were observed in the surface soil and in the planting row, and were strongly related to fertilizer rate. Samples taken between the planting rows did not exhibit treatment effects on available P. The higher values of available P obtained with Mehlich‐1 and the lower eucalyptus plant uptake efficiency of fertilizer‐P from banded RP confirms the fact that this extractant can overestimate the availability of P in soils receiving RP. The use of anion exchange resin in this situation to estimate available P is supported. The results obtained with the localized application of RP indicate root system activity (P and Ca uptake and acidification of rhizosphere) as a factor in increasing fertilizer dissolution rates.

Adsorption and Desorption of Arsenate in Different Soils and Gold Mining Substrates of Minas Gerais State, Brazil

BackgroundArsenic (As) availability in natural environment is related to the element’s adsorption and desorption processes in soils. Total As is better related to available As in temperate soils than in tropical soils. In tropical soils, total As is not very significant in terms of availability, therefore justifying the necessity for studies into As dynamics. Knowledge of As dynamics in soil as well as development of new analytical methodologies involving tropical soils are insufficient and necessary for future mitigation projects.ObjectiveThe objectives of this study were: (1) To adjust methodologies which may assist in understanding arsenate dynamics in tropical soils and substrates; (2) To evaluate the adsorption and desorption of arsenate in soils and substrate samples, and to find a minimum value of arsenate available in soil which is lethal to sorghum plants.Material and MethodsSamples of three soils from Minas Gerais State (YL, RYL, and CS) and two sulfide substrates of gold mining (B1 and B2) were used in the assays. All the material was physically and chemically characterized. Remaining As (As-rem) and remaining P (P-rem) of each material, along with MACP and MACAs (using the Langmuir isotherms), were obtained. After agitation to obtain MACP and MACAs, arsenate was extracted by anionic resin and Mehlich-III to evaluate arsenate desorption of the material retained on the filter paper. Subsequently, arsenate desorption curves for the different materials were obtained, and arsenate availability was determined through a bioassay with sorghum plants. Samples of soils and substrate B1 were incubated with six levels of As doses. Plants were grown under greenhouse conditions for 30 days. The plants were then harvested, dried and weighed. Available As in the soils and substrate was determined by Mehlich-III.Results and DiscussionsAs-rem level decreased from YL (sandy) to RYL (clayey) soil samples, which always showed lower values than P-rem. Among the soils and substrates evaluated, RYL showed the highest MACAs and MACP, followed by CS, YL and Bl. The results were in accordance with the values observed for As-rem and P-rem and confirm the idea that the ability of the assayed materials to remove As from the soil/substrate solution is higher than the ability to remove P. On the other hand, the binding energy (a) between soil/substrate and As is weaker than the binding energy of P. Given the fact that the studied soils present a real ability to remove As from the solution, only a small part of As would be unavailable considering MACAs as a reference. As-Mehlich-III values were higher than As-resin for substrate Bl. Mehlich-III seemed to be more appropriate to extract labile forms of arsenate in substrate B1 as well as in the soils. Available As by Mehlich-III (26.9 mg/dm3) was considered a reference of As LCL to sorghum plants. CC50 was sensitive to the buffering capacity of each soil, showing values varying from 1.34 mg/dm3 As (clay soil with lower As-rem) to 12.31 mg/dm3 As (sandy soil with higher As-rem).ConclusionsThe adaptation of the As-rem and MACAs methodologies was satisfactory and of great value in the study of adsorption, desorption and As availability for soils and mining substrate. Mehlich-III was also satisfactory to estimate available As and was sensitive to soil buffering capacity. Nevertheless, resin can also be used as an alternative. MACAs varied among soils and was higher than MACp. However, As showed higher lability than P. Using Mehlich-III, we determined the value corresponding to CC50 that showed a good reference of toxicity to available As.OutlookThe environmental implications of the As behavior are quite serious. Beside the fact that arsenate is removed very fast from the soil solution, an anthropogenic input of the element, being part of the soil quantity factor, may remain in a reversible form for a long time. As may therefore return to the soil solution and becomes available to plants, animals and the entire environment. Considering that CC50 is the maximum contents of available As the environment can tolerate to allow some vegetal biomass production, the maximum capacity of As immobilization in each soil is reduced when compared to the soils’ MACAs values. Therefore, the maximum and safe values of reference to be used in the evaluation of incidental discharge of the element in soils must be reduced.

Arsenic toxicity in Acacia mangium willd. and mimosa Caesalpiniaefolia benth. seedlings

Acacia mangium and Mimosa caesalpiniaefolia are fast-growing woody fabaceous species that might be suitable for phytoremediation of arsenic (As)-contaminated sites. To date, few studies on their tolerance to As toxicity have been published. Therefore, this study assessed As toxicity symptoms in A. mangium and M. caesalpiniaefolia seedlings under As stress in a greenhouse. Seedlings of Acacia mangium and M. caesalpiniaefolia were grown for 120 d in an Oxisol-sand mixture with 0, 50, 100, 200, and 400 mg kg-1 As, in four replications in four randomized blocks. The plants were assessed for visible toxicity symptoms, dry matter production, shoot/root ratio, root anatomy and As uptake. Analyses of variance and regression showed that the growth of A. mangium and M. caesalpiniaefolia was severely hindered by As, with a reduction in dry matter production of more than 80 % at the highest As rate. The root/shoot ratio increased with increasing As rates. At a rate of 400 mg kg-1 As, whitish chlorosis appeared on Mimosa caesalpiniaefolia seedlings. The root anatomy of both species was altered, resulting in cell collapse, death of root buds and accumulation of phenolic compounds. Arsenic concentration was several times greater in roots than in shoots, with more than 150 and 350 mg kg-1 in M. caesalpiniaefolia and A. mangium roots, respectively. These species could be suitable for phytostabilization of As-contaminated sites, but growth-stimulating measures should be used.

POTENTIAL OF THREE LEGUME SPECIES FOR PHYTOREMEDIATION OF ARSENIC CONTAMINATED SOILS 1

Phytoremediation strategies utilize plants to decontaminate or immobilize soil pollutants. Among soil pollutants the metalloid arsenic (As) is the one of primary concern. Elevated soil As results from anthropogenic activities such as use of pesticides (herbicides and fungicides), use of certain fertilizers, metal mining, iron and steel production, coal combustion, and from co- production during natural gas extraction. This study evaluated the potential of pigeon pea (Cajanus cajan), wand riverhemp (Sesbania virgata), and lead tree (Leucaena leucocephala) for phytoremediation of soils polluted by As. Soil samples were placed in plastic pots, incubated with different As doses (0, 50, 100 and 200 mg dm -3 ) and then sown with seeds of these three species. Ninety days after sowing, the plants were evaluated for height, collar diameter and dry matter of young, intermediate and basal leaves, stems and roots. Arsenic concentration was determined in different aged leaves, stems and roots to establish the translocation index (TI) between plant roots and aerial plant components. The evaluated species showed distinctly different characteristics with respect to As tolerance, since the lead tree and wand riverhemp were significantly more tolerant than pigeon pea. High As levels found in wand riverhemp roots suggest the existence of an effective mechanism of accumulation and compartmentalization in order to reduce As translocation to aboveground tissues. Pigeon pea is a sensitive species and could serve as a potential bioindicator plant, whereas the other two species have potential for phytoremediation programs in As polluted areas. However, further studies are needed with longer exposure times in actual field conditions to reach definitive conclusions on the relative phytoremediation potentials on these species.

Induction of a Geochemical Barrier for As, Fe and S Immobilization in a Sulfide Substrate

Acid mine drainage (AMD) is an environmental concern due to the risk of element mobilization, including toxic elements, and inclusion in the food chain. In this study, three cover layers were tested to minimize As, Fe and S mobilization from a substrate from former gold mining, containing pyrite and arsenopyrite. For this purpose, different layers (capillary break, sealant and cover layer) above the substrate and the induction of a geochemical barrier (GB) were used to provide suitable conditions for adsorption and co-precipitation of the mobilized As. Thirteen treatments were established to evaluate the leaching of As, Fe and S from a substrate in lysimeters. The pH, As, Fe, S, Na, and K concentrations and total volume of the leachates were determined. Mineralogical analyses were realized in the substrate at the end of the experimental period. Lowest amounts of As, Fe and S (average values of 5.47, 48.59 and 132.89 g/lysimeter) were leached in the treatments that received Na and K to induce GB formation. Mineralogical analyses indicated jarosite formation in the control treatment and in treatments that received Na and K salts. However, the jarosite amounts in these treatments were higher than in the control, suggesting that these salts accelerated the GB formation. High amounts of As, Fe and S (average values of 11.7, 103.94 and 201.13 g/lysimeter) were observed in the leachate from treatments without capillary break layer. The formation of geochemical barrier and the use of different layers over the sulfide substrate proved to be efficient techniques to decrease As, Fe and S mobilization and mitigate the impact of acid mine drainage.