Lukas Capek

Influence of the orientation of the Osstell transducer during measurement of dental implant stability using resonance frequency analysis: a numerical approach.

Stability of dental implants is measured by means of the Osstell device using a method of resonance frequency analysis. The aim of the study was to evaluate the role of direction-dependence of the Osstell transducer. For this purpose, a set of parametrical finite element analyses has been used. When rotating the transducer around the vertical axis the crucial phenomenon is the change in behavior of the system, which occurs in positions between 30 degrees and 80 degrees to the long axis of the alveolar crest. It seems from the presented results that, when measuring approximately perpendicularly to this long axis, the deviation from the ideal perpendicular position must not exceed 30 degrees. In this case, the first resonance frequency is recorded. When measuring in the approximately parallel position to the long axis of the alveolar crest, the second resonance frequency is recorded. The deviation from the ideal parallel position must not exceed 10 degrees. These values are not comparable with the previous ones.

Evaluation of acetabular cup initial fixation by using resonance frequency principle

The clinical practice shows that the loosening of acetabular cups is more frequent than stem loosening. With standard cups, the incidence of dislocation failure is highest in the first year after arthroplasty implantation. The aim of the study was to quantitatively evaluate the implant-bone stability of a cementless acetabular cup prosthesis by using a device based on resonance frequency analysis. The evaluation of this device was done by finite element analysis and in vitro experiments. It was shown that not all the resonance frequencies can be measured by our device. The resonance frequencies vary within the range of 500–3000 Hz. The proposed power spectrum measurement gives the information about the absolute stiffness of the press-fit implant.

Different inhibition of acetylcholinesterase in selected parts of the rat brain following intoxication with VX and Russian VX

Differences between acetylcholinesterase (AChE) inhibition in the brain structures following VX and RVX exposure are not known as well as information on the possible correlation of biochemical and histochemical methods detecting AChE activity. Therefore, inhibition of AChE in different brain parts detected by histochemical and biochemical techniques was compared in rats intoxicated with VX and RVX. AChE activities in defined brain regions 30 min after treating rats with VX and Russian VX intramuscularly (1.0 × LD50) were determined by using biochemical and histochemical methods. AChE inhibition was less expressed for RVX, in comparison with VX. Frontal cortex and pontomedullar areas containing ncl. reticularis has been found as the most sensitive areas for the action of VX. For RVX, these structures were determined to be frontal cortex, dorsal septum, and hippocampus, respectively. Histochemical and biochemical results were in good correlation (Rxy = 0.8337). Determination of AChE activity in defined brain structures was a more sensitive parameter for VX or RVX exposure than the determination of AChE activity in the whole-brain homogenate. This activity represents a “mean” of the activities in different structures. Thus, AChE activity is the main parameter investigated in studies searching for target sites following nerve-agent poisoning contributing to better understanding of toxicodynamics of nerve agents.

Combined approach to demonstrate acetylcholinesterase activity changes in the rat brain following tabun intoxication and its treatment

Reactivation effects of K203 and currently available oximes (obidoxime, HI-6) in combination with atropine on acetylcholinesterase activities in the brain parts of rats poisoned with tabun were studied. The activity was determined by quantitative histochemical and biochemical methods correlating between them very well. The tabun-induced changes in acetylcholinsterase activity as well as in reactivation potency of reactivators used were different in various parts of the brain. Pontomedullar area seems to be important for observed changes following tabun intoxication and its treatment. From the oximes studied, the reactivation effect of K203 was comparable with obidoxime; HI-6 was ineffective. Combination of bio- and histochemical methods allow fine differentiation among the action of different oximes following tabun poisoning.

Impact Force, Polar Gap and Modal Parameters Predict Acetabular Cup Fixation: A Study on a Composite Bone

The balanced initial fixation of an implant makes up a crucial condition for its long-term survival. However, the quantification of initial fixation is no easy task and, to date, only qualitative assessments can be made. Although the concept of measuring fixation by means of vibration analysis is already widely used in dental implantology, the rigorous application of this method for the assessment of the fixation of femoral and acetabular components remains a challenge. Moreover, most studies on this subject have tended to focus solely on the femoral stem even though acetabular cup fixation is also important and even more difficult with respect to qualitative measurement. This study describes a comprehensive experiment aimed at assessing acetabular cup fixation. Fixation is expressed in terms of the impact force and polar gap variables, which are correlated with the modal properties of the acetabular implant during the various insertion stages. The predictive capabilities of modal frequencies and frequency functions were investigated by means of surrogate models based on the Gaussian process and functional principal component analysis. The prediction accuracy of the proposed models was in the range 82–94%. The results indicate that natural frequencies, reduced frequency, impact force and polar gap features provide great potential in terms of the prediction of implant fixation.

A method of scar evaluation using non-contact 3D scanner

After the skin is injured, the healing process usually leaves a flat scar. Keloid is a special type of scar that develops on the wound and has a pink or purple colour. Keloids are irregularly shaped and tend to enlarge progressively (Cole 2012). Keloids (Figure 1) typically appear following surgery or injury, but they can also appear spontaneously or as a result of inflammation, such as acne (Cole 2012). Keloid scars can be up to 15 times more common in people with darker skin types than in Caucasian population (Taylor et al. 2007). This study was done as a next step of the research on the impact of tissue adhesives on scar creation (Mitbauerová et al. 2012). Tissue adhesives can be applied in various areas and knowledge of their mechanical properties could help in their broader usage. The aim of this part of the study was to develop an effective method on how to measure the comparable properties of resulting scars, which were closed with tissue adhesive or suture. Based on this research, the scar assessment process could be improved as is expected so that the volume of a keloid scar should decrease with successful treatment (Taylor et al. 2007). Experiments consist of measuring patients with keloid scars via NextEngine 3D Scanner and analysing resulting data in GeoMagic 3D software.

Experimental and numerical analysis of a V-Y advancement flap on human skin ex vivo

D. Remache*, J. Pauchot, J. Chambert, L. Capek and E. Jacquet Department of Applied Mechanics, University of Franche-Comte, Femto-st Institute – UMR 6174 CNRS, 24 rue de l’ Epitaphe, 25000 Besançon, France; Orthopaedic, Traumatology, Plastic Reconstructive and Hand Surgery Unit, EA 4268I4S IFR133 INSERM, University Hospital of Besançon, Place Saint-Jacques, F-25030 Besançon, France; Department of Mechanical Engineering, Technical University of Liberec, Studenska 2, 46117 Liberec 1, Czech Republic

A comparison of tabun-inhibited rat brain acetylcholinesterase reactivation by three oximes (HI-6, obidoxime, and K048) in vivo detected by biochemical and histochemical techniques.

Tabun belongs to the most toxic nerve agents. Its mechanism of action is based on acetylcholinesterase (AChE) inhibition at the peripheral and central nervous systems. Therapeutic countermeasures comprise administration of atropine with cholinesterase reactivators able to reactivate the inhibited enzyme. Reactivation of AChE is determined mostly biochemically without specification of different brain structures. Histochemical determination allows a fine search for different structures but is performed mostly without quantitative evaluation. In rats intoxicated with tabun and treated with a combination of atropine and HI-6, obidoxime, or new oxime K048, AChE activities in different brain structures were determined using biochemical and quantitative histochemical methods. Inhibition of AChE following untreated tabun intoxication was different in the various brain structures, having the highest degree in the frontal cortex and reticular formation and lowest in the basal ganglia and substantia nigra. Treatment resulted in an increase of AChE activity detected by both methods. The highest increase was observed in the frontal cortex. This reactivation was increased in the order HI-6 < K048 < obidoxime; however, this order was not uniform for all brain parts studied. A correlation between AChE activity detected by histochemical and biochemical methods was demonstrated. The results suggest that for the mechanism of action of the nerve agent tabun, reactivation in various parts of the brain is not of the same physiological importance. AChE activity in the pontomedullar area and frontal cortex seems to be the most important for the therapeutic effect of the reactivators. HI-6 was not a good reactivator for the treatment of tabun intoxication.

Failure of sternal wires depends on the number of turns and plastic deformation: combined experimental and computational approach

OBJECTIVES The number of turns at the end of a wire closure is not described or discussed in any cardiosurgical guidelines. The hands-on experience of the surgeon plays a significant role. The aim of this work was to clarify the relationship between the number of turns of the suture and the resulting strength of the sternal fixation. METHODS The study was performed in 2 independent steps. The first step was a finite element simulation, where the stress and strain distribution of the sternal fixation was observed. The second step included the experimental set-up and the statistical evaluation of the results. RESULTS Our study showed that the failure force rose linearly as the number of turns increased. The lowest average measured force was 370 N (3 turns); the highest was 430 N (7 turns). The failure modes were either untwisting of the wires or rupture of the closure, which is controlled by the number of turns. As the number of turns increases, superficial cracks can occur. CONCLUSIONS Based on our results, the 5-turn option is the best solution for the closure. The failure force is still double the value reported in the literature, so there is a high safety margin for failure. The failure mode is untwisting; hence, no unexpected fracture can occur, and there is still an elastic core in the cross-section of the wire.

How does the surface treatment change the cytocompatibility of implants made by selective laser melting

ABSTRACT Introduction: The study investigates the potential for producing medical components via Selective Laser Melting technology (SLM). The material tested consisted of the biocompatible titanium alloy Ti6Al4V. The research involved the testing of laboratory specimens produced using SLM technology both in vitro and for surface roughness. The aim of the research was to clarify whether SLM technology affects the cytocompatibility of implants and, thus, whether SLM implants provide suitable candidates for medical use following zero or minimum post-fabrication treatment. Areas covered: The specimens were tested with an osteoblast cell line and, subsequently, two post-treatment processes were compared: non-treated (as-fabricated) and glass-blasted. Interactions with MG-63 cells were evaluated by means of metabolic MTT assay and microscope techniques (scanning electron microscopy, fluorescence microscopy). Surface roughness was observed on both the non-treated and glass-blasted SLM specimens. Expert Commentary: The research concluded that the glass-blasting of SLM Ti6Al4V significantly reduces surface roughness. The arithmetic mean roughness Ra was calculated at 3.4 µm for the glass-blasted and 13.3 µm for the non-treated surfaces. However, the results of in vitro testing revealed that the non-treated surface was better suited to cell growth.