Lukasz M. Karbowski

Digitized quantitative electroencephalographic patterns applied as magnetic fields inhibit melanoma cell proliferation in culture

Weak (1 μT) physiologically patterned magnetic fields produce changes in behavioral, physiological, and cellular activity. In the present experiments 12 temporal samples of the electroencephalographic anomaly and normal activity of a person (SLH) whose proximity reliably affected the brain activity of others were extracted from QEEG data, digitized, and presented as equivalent magnetic field patterns to B16 mouse melanoma cells. Only two of the patterns, both originating from the primary source (right temporal lobe) of the EEG anomaly reduced the cell growth by one-third compared to the other patterns extracted from his QEEG or sham field exposures. In previous experiments these EEG transients were also associated with marked increases in photon emissions from the right side of SLHs head. The results suggest that the intrinsic complexity of electroencephalographic patterns of some people, when amplified appropriately and applied as computer-generated magnetic fields in the three spatial planes, could diminish cancer cell growth.

Temporally-Patterned Magnetic Fields Induce Complete Fragmentation in Planaria

A tandem sequence composed of weak temporally-patterned magnetic fields was discovered that produced 100% dissolution of planarian in their home environment. After five consecutive days of 6.5 hr exposure to a frequency-modulated magnetic field (0.1 to 2 µT), immediately followed by an additional 6.5 hr exposure on the fifth day, to another complex field (0.5 to 5 µT) with exponentially increasing spectral power 100% of planarian dissolved within 24 hr. Reversal of the sequence of the fields or presentation of only one pattern for the same duration did not produce this effect. Direct video evidence showed expansion (by visual estimation ∼twice normal volume) of the planarian following the first field pattern followed by size reduction (estimated ∼1/2 of normal volume) and death upon activation of the second pattern. The contortions displayed by the planarian during the last field exposure suggest effects on contractile proteins and alterations in the cell membrane’s permeability to water.

Inverse relationship between photon flux densities and nanotesla magnetic fields over cell aggregates: Quantitative evidence for energetic conservation

The quantitative relationship between local changes in magnetic fields and photon emissions within ∼2 mm of aggregates of 105–106 cells was explored experimentally. The vertical component of the earths magnetic field as measured by different magnetometers was ∼15 nT higher when plates of cells removed from incubation were measured compared to plates containing only medium. Additional experiments indicated an inverse relationship over the first ∼45 min between changes in photon counts (∼10−12W·m−2) following removal from incubation and similar changes in magnetic field intensity. Calculations indicated that the energy within the aqueous volume containing the cells was equivalent for that associated with the flux densities of the magnetic fields and the photon emissions. For every approximately 1 nT increase in magnetic field intensity value there was a decrease of ∼2 photons (equivalent of 10−18J). These results complement correlation studies and suggest there may be a conservation of energy between expression as magnetic fields that are subtracted or added to the adjacent geomagnetic field and reciprocal changes in photon emissions when aggregates of cells within a specific volume of medium (water) adapt to new environments.

Novel Cosic resonance (standing wave) solutions for components of the JAK–STAT cellular signaling pathway: A convergence of spectral density profiles

Cosic discovered that spectral analyses of a protein sequence after each constituent amino acid had been transformed into an appropriate pseudopotential predicted a resonant energy between interacting molecules. Several experimental studies have verified the predicted peak wavelength of photons within the visible or near‐visible light band for specific molecules. Here, this concept has been applied to a classic signaling pathway, JAK–STAT, traditionally composed of nine sequential protein interactions. The weighted linear average of the spectral power density (SPD) profiles of each of the eight “precursor” proteins displayed remarkable congruence with the SPD profile of the terminal molecule (CASP‐9) in the pathway. These results suggest that classic and complex signaling pathways in cells can also be expressed as combinations of resonance energies.

Post-seizure drug treatment in young rats determines clear incremental losses of frontal cortical and hippocampal neurons: The resultant damage is similar to very old brains

Loss of neurons occurs with aging and following lithium/pilocarpine-induced epileptic seizures. In the present study, the numbers of neurons within the layers from sample areas of the four lobes of the neocortices and the hippocampus were counted by light microscopy in brains of rats that had been administered lithium or pilocarpine and then injected immediately or shortly after seizure onset with either acepromazine, ketamine, or prazosin. The mean numbers of neocortical and hippocampal neurons were lowest in rats treated with acepromazine or prazosin 1h after seizure onset, while those of rats immediately treated with ketamine displayed the least decrements and were most similar to normal rats. The largest loss of neurons occurred within the CA1 field and layers 5 and 6 of the frontal cortices. The mean numbers of neurons within the cortices in rats whose treatments had been delayed for 1h were similar to those of normal rats over 700 days of age. These results support the hypothesis that neuronal loss from cumulative effects of seizure-induced brain damage simulates aging.

Synergistic interactions between temporal coupling of complex light and magnetic pulses upon melanoma cell proliferation and planarian regeneration

ABSTRACT Synergisms between a physiologically patterned magnetic field that is known to enhance planarian growth and suppress proliferation of malignant cells in culture and three light emitting diode (LED) generated visible wavelengths (blue, green, red) upon planarian regeneration and melanoma cell numbers were discerned. Five days of hourly exposures to either a physiologically patterned (2.5–5.0 μT) magnetic field, one of three wavelengths (3 kLux) or both treatments simultaneously indicated that red light (680 nm), blue light (470 nm) or the magnetic field significantly facilitated regeneration of planarian compared to sham field exposed planarian. Presentation of both light and magnetic field conditions enhanced the effect. Whereas the blue and red light diminished the growth of malignant (melanoma) cells, the effect was not as large as that produced by the magnetic field. Only the paired presentation of the blue light and magnetic field enhanced the suppression. On the other hand, the changes following green light (540 nm) exposure did not differ from the control condition and green light presented with the magnetic field eliminated its effects for both the planarian and melanoma cells. These results indicate specific colors affect positive adaptation that is similar to weak, physiologically patterned frequency modulated (8–24 Hz) magnetic fields and that the two forms of energy can synergistically summate or cancel.

Combined therapeutic use of oral alitretinoin and narrowband ultraviolet-B therapy in the treatment of Hailey-Hailey disease

Hailey-Hailey disease (HHD) is a chronic familial bullous disease characterized by recurrent blisters and erosions typically at friction-prone areas of the body accompanied by acantholysis upon histologic examination. There are a number of therapies used in the management of HHD. Its symptoms have been effectively treated with antimicrobial therapies, corticosteroids and other agents such as cyclosporine and prednisone. However, such treatments are not always effective. Therefore, there is a need for new treatments for the management of HHD. In this report, a patient with long-standing HHD responsive only to high levels of prednisone is described. After the successful tapering and cessation of oral prednisone the patient began a new combination therapy of complementary doses of oral alitretinoin, and narrowband UVB therapy, which yielded a favorable response within 2-3 weeks. After 6 weeks, a mono-therapy of daily (30 mg) oral alitretinoin was sufficient to maintain successful near-complete remission of the disease.

Experimental Evidence of Classical Conditioning and Microscopic Engrams in an Electroconductive Material

Synthetic experimental substrates are indispensable tools which can allow researchers to model biological processes non-invasively in three-dimensional space. In this study, we investigated the capacities of an electroconductive material whose properties converge upon those of the brain. An electrically conductive material composed of carbohydrates, proteins, fats, ions, water, and trace amounts of other organic compounds and minerals was classically conditioned as inferred by electrophysiological measurements. Spectral densities evoked during the display of a conditioned stimulus (CS) probe were strongly congruent with those displayed during the conditioned-unconditioned stimulus pairing (CS-UCS). The neutral stimulus consisted of the pulsed light from a LED. The unconditioned stimulus was an alternating current. Interstimulus intervals >130 ms did not result in conditioned responses. Microscopic analysis of the chemically-fixed substratum revealed 10–200 μm wide ‘vessel structures’ within samples exposed to a stimulus. Greater complexity (increased fractal dimensions) was clearly discernable by light microscopy for stained sections of fixed samples that had been conditioned compared to various controls. The denser pixels indicated greater concentration of stain and increased canalization. Implications for learning and memory formation are discussed.

Differentiation of Malignant Compared to Non-Malignant Cells by TheirBio-Photon Emissions May Only Require a Specific Filter around 500 nm

Emphasis upon early detection of malignant cellular growths rather than imaging could allow earlier intervention. Photon emissions from malignant cells even when they constitute a very small proportion of the normal organ has been shown to require a technical understanding of the spectral power density profiles that can be predicted by Cosic’s Molecular Resonance Recognition equation. Here we demonstrate experimentally a simpler more robust detection method involving specific filters of photon emissions from cells in culture. Photons from human pancreatic malignant cancer cells displayed conspicuously suppressed spikes of photons within a narrow band (500 nm) but not at 370 nm, 420 nm, 620 nm, 790 nm, or 950 nm increments compared to non-malignant human embryonic kidney cells. Given the recent demonstration that malignant cells can “store” photons within a specific wavelength when pulsed at the same pattern as a yoked magnetic field and re-emit the photons in this wavelength tens of minutes later, diminishment of power within specific 10 nm increments of visible wavelength spectra may serve as an early detection of imminent malignancy.

Spectral Power Densities and Whole Body Photon Emissions from Human Subjects Sitting in Hyper-darkness

The human body emits a continuous field of photons that may exhibit holographic-like properties. If this concept is applicable then the appropriate technology and quantitative methods would have the capacity to detect anomalous sources anywhere within the volume of the body. To discern the feasibility of this concept we tested the capacity of four photomultiplier units to discriminate the presence or absence of a human being within a hyperdark (10-12 W·m-2) small room specifically constructed for this purpose. Only 100 s of measurements of photon emissions (50 Hz sampling, 20 ms bins) were required to obtain 100% accurate discrimination. Spectral Power Densities (SPD) for the photon counts when human subjects were present or not present were sufficiently complex to allow potential discernment of different health states. Preliminary data have already suggested that this particular method has the potential to function as a sensi