Lutfi Tunc

Stones in anomalous kidneys : Results of treatment by shock wave lithotripsy in 150 patients

Objective:u2002 To determine the efficacy of shock wave lithotripsy (SWL) in anomalous kidneys.

Can serum Inhibin B and FSH levels, testicular histology and volume predict the outcome of testicular sperm extraction in patients with non-obstructive azoospermia?

IntroductionIn our study, we evaluated the diagnostic accuracy of serum follicle stimulating hormone (FSH), Inhibin B, testicular volumes and distribution of testicular sperm extraction (TESE) outcome according to the histological diagnosis in men with non-obstructive azoospermia.Materials and methodsBetween February 2001 and April 2002, 66 men presenting with infertility of at least 1xa0year were found to have non-obstructive azoospermia. Serum FSH and Inhibin B levels, testicular volumes and pathological analysis were reviewed retrospectively using medical records of these patients.ResultsOf 66 patients, 52 were enrolled into the study and sperm extraction was successful in 31 of 52 patients (59.6%). There was no statistically significant difference between the patients who had successful and unsuccessful TESE in terms of mean serum Inhibin B, FSH levels and testicular volumes (Pxa0>xa00.05). The area under ROC analysis for serum Inhibin, serum FSH and testicular volume was 0.557, 0.523 and 0.479, respectively. For Inhibin B, the best cut-off value for discriminating between successful and failed TESE at 90% sensitivity was 6.25 with a very low level of specificity (14%) and diagnostic accuracy that was 53.8.ConclusionBesides the controversies about the direct marker role of serum Inhibin B in determination of spermatogenesis, it does not seem to give a clue about the prediction of sperm presence before TESE. Because of the conflicting results in the literature, the potential role of serum Inhibin B as a marker for prediction of sperm presence in testis is yet to be determined.

Comparison of retrograde intrarenal surgery and mini-percutaneous nephrolithotomy in management of lower-pole renal stones with a diameter of smaller than 15 mm.

The aim of this study was to compare the outcomes of retrograde intrarenal surgery (RIRS) and miniaturized percutaneous nephrolithotomy (mini-PNL) in management of lower-pole renal stones with a diameter smaller than 15xa0mm. Between December 2009 and July 2012, the patients with the diagnosis of lower-pole stones were evaluated by ultrasonography, intravenous pyelography and computed tomography. The records of 73 evaluable patients who underwent mini-PNL (nxa0=xa037) or RIRS (nxa0=xa036) for lower-pole (LP) stones with diameter smaller than 15xa0mm were reviewed retrospectively. Of the 73 patients, 37 underwent mini-PNL and 36 underwent RIRS. The stone-free rates were 89.1 and 88.8xa0% for mini-PNL and RIRS groups, respectively. The mean operation time was 53.7xa0±xa014.5 in the mini-PNL group but 66.4xa0±xa015.8 in the RIRS group (Pxa0=xa00.01). The mean fluoroscopy times and hospitalization times were significantly higher in the mini-PNL group. There was no major complication in any patient. RIRS and mini-PNL are safe and effective methods for treatment of LP calculi with a diameter smaller than 15xa0mm. RIRS is a non-invasive and feasible treatment option, and has also short hospitalization time, low morbidity and complication rate. It may be an alternative of mini-PNL in the treatment LP calculi with smaller than 15xa0mm.

Pneumatic lithotripsy for large ureteral stones: is it the first line treatment?

ObjectiveTo evaluate the effectiveness of pneumatic lithotripsy (PL) with ureteroscopy in the treatment of large ureteral stones.MethodsWe reviewed, retrospectively, the records of 156 patients (122 male, 34 female) who had ureteral calculi larger than 10xa0mm that were treated with PL. Of these patients, 41 (26.3%) were treated primarily with PL and 115 (73.7%) were treated secondarily after unsuccessful extracorporeal shock wave lithotripsy (SWL). The mean stone diameter was 12.87xa0mm (range 10–20.5xa0mm). Results were evaluated 3xa0months after treatment by excretory urography and/or ultrasonography.ResultsThe overall stone-free and fragmentation rates (FRs) were 85.2 and 92.3%, respectively. Corresponding values were 60 and 84% for upper ureteral stones, 79.5 and 89.7% for middle ureter stones and 94.5 and 95.6% for lower ureteral stones, respectively. The main complications were migration of a complete stone or of fragments (7.1%), urosepsis (4.5%) and ureteral perforation (1.3%).ConclusionsAlthough SWL is generally accepted as the first treatment option for ureteral stones because of its non-invasive nature, PL with ureteroscopy seems to be a good alternative with the advantage of higher success rates and quick stone clearance. Especially when we take the importance of quick stone removal into account for larger ureteral stones, which are more likely to have obstruction, impaction, or infection, we believe that PL may be chosen as the first line treatment rather than SWL for stones larger than 10xa0mm.

Value of Ultrasonography and Helical Computed Tomography in the Diagnosis of Stone-Free Patients after Extracorporeal Shock Wave Lithotripsy (USG and Helical CT after SWL)

Aim: To define the value of different radiologic modalities in determining the patients who believed to be stone-free after extracorporeal shock wave lithotripsy (SWL) with plain abdominal X-ray, by evaluating the same patients with ultrasonography (USG) and helical computed tomography (CT). Patients and methods: Between March 2002 and February 2003, 76 patients with urolithiazis who were treated with SWL and considered to be stone-free with plain abdominal X-ray, were evaluated with USG and helical CT. The results were compared for the accuracy of the stone-free diagnosis. Results: Residual stones were detected in 9 (11.8%) with USG and in 17 (22.3%) with CT of 76 patients who were thought to be stone-free with plain abdominal X-ray alone. Conclusions: Although plain abdominal X-ray has been accepted as the first line diagnostic tool in the follow-up after SWL with its cheap and practical use, helical CT was found to be more valuable in diagnosis of residual stone fragments which has not been found in plain abdominal X-ray. If we take these considerations which can change our clinical approach and patient follow-up into account, we believe that the routine use of helical CT can give more accurate information in patient controls after SWL.

POWER DOPPLER ULTRASOUND MAPPING IN NONOBSTRUCTIVE AZOOSPERMIC PATIENTS PRIOR TO TESTICULAR SPERM EXTRACTION

This study was conducted to localize the testicular regions, which have better blood circulation by power Doppler ultrasonography in patients with nonobstructive azoospermia before testicular sperm extraction (TESE), and to investigate whether these vascularized areas have a high sperm retrieval rate or not. We evaluated 110 testes of 55 cases that were diagnosed as nonobstructive azoospermia. The mean age of the study group was 33 years (range 26 to 42). Patients with Y chromosome microdeletions, karyotype and hormonal abnormalities (except elevated FSH levels) were excluded from the study. In all cases, testes were evaluated by power Doppler ultrasonography before testicular sperm extraction. Testis was divided vertically into five equal parts and the area with maximum vascularity was determined subjectively. During testicular sperm extraction, starting from best-perfused areas, biopsies were done. If no motile or sufficient amount of sperm was found, TESE procedure was tried on the contralateral testis. TESE were performed from 82 testes and for the regions that show good and poor vascularity. The sperm finding rate was 38% and 14%, respectively (OR = 3.55)(p = 0.001). Power Doppler ultrasound mapping of the testis in nonobstructive azoospermic cases is a reliable and informative method to assess spermatogenic foci. It is a noninvasive technique that minimizes the unnecessary removal of hormone producing tissue and gives chance to end the TESE earlier than currently practiced procedures.

In vivo Porcine Model for Practicing Retrograde Intrarenal Surgery

Objectives: To examine the feasibility of retrograde intrarenal surgery (RIRS) in a porcine model. Materials and Methods: Female pigs (n = 3) were placed in a dorsal lithotomy position under general anesthesia, and stone material was inserted into the renal pelvis of the pigs. The bladder was entered with a cystoscope, and a 0.038-inch hydrophilic guidewire was passed into the renal pelvis. Following successful placement of the guidewire, a ureteral access sheath (9.5/11.5 Fr) was placed to allow for optimal visualization. A 7.5-Fr flexible ureteroscope (Karl Storz Flex-X2) and a 200-μm laser fiber were used for lithotripsy. When basketing was deemed necessary, zero-tipped nitinol stone baskets were used. Trainees then practiced all these manipulations on the model. Results: Urologists with moderate experience in advanced endourologic surgery were trained using this model. However, there were some surgical difficulties due to the urinary system anatomy of the pig. Intravaginal location of the urethra, bladder neck location of the ureters, tight ureteric orifices, tortuous ureters, longitudinally elongated renal pelvis, narrow infundibulopelvic angle and shallow calices made the passage of the instruments and maneuverability of the flexible ureteroscope more difficult than in a human model. Conclusions: Despite some difficulties, our porcine model was very effective, because all the trainees successfully practiced the RIRS manipulations on this model.

The impact of pelvicaliceal anatomical variation between the stone-bearing and normal contralateral kidney on stone formation in adult patients with lower caliceal stones

OBJECTIVEnWe aimed to investigate the effect of pelvicaliceal anatomical differences on the etiology of lower caliceal stones.nnnMATERIALS AND METHODSnRecords of adult patients between January 1996 and December 2005 with solitary lower caliceal stone were reviewed. After exclusion of patients with hydronephrosis, major renal anatomic anomalies, non-calcium stones, history of recurrent stone disease and previous renal surgery, 78 patients were enrolled into the study. Lower pole infundibulopelvic angle (IPA), infundibulovertebral angle (IVA), infundibular length (IL), width (IW), number of minor calices and cortical thickness of the lower pole together with other caliceal variables obtained from the whole pelvicaliceal anatomy of both stone-bearing and contralateral normal kidneys were measured from intravenous pyelogram of the patients. Total pelvicaliceal volume was also calculated by a previously described formula for both kidneys.nnnRESULTSnThere were statistically significant difference between two kidneys in terms of IW (p < 0.001) and IL (p = 0.002) of the upper calyx, IW (p = 0.001) and IVA (p < 0.001) of the lower calyx), pelvicaliceal volume (p < 0.001), IPA of middle calyx (p = 0.006) and cortical thickness over the lower pole (p < 0.001). However there was no difference between stone-bearing and contralateral normal kidneys in terms of lower pole IPA (p = 0.864) and IL (p = 0.568).nnnCONCLUSIONnPelvicaliceal volume but not lower caliceal properties seem to be a risk factor for stone formation in lower calyx.

Effects of chronic treatment with valproate and oxcarbazepine on testicular development in rats.

PURPOSEnThe aim of this study was to examine the potential effects of valproate (VPA) and oxcarbazepine (OXC) on testicular development in rats.nnnMETHODSnForty-two Wistar rats were randomly divided into three groups of 14 rats each. Each group received the following via gavage over 90 days: group 1, tap water (control group); group 2, VPA (300mg/kg/day); group 3, OXC (100mg/kg/day). After sacrifice, body, testicular and epididymidis weights were measured. Testes were sampled, fixed and processed, and quantitative morphometric analysis of Sertoli cells, spermatocytes and spermatids was performed in stages II, V and XII by histopathological examination. Immunohistochemical staining was performed to transform growth factor beta 1 (TGF-β1) and p53, and the apoptotic index was assessed using the TUNEL method.nnnRESULTSnTestis and relative testis weights were significantly lower in the VPA group compared to the control group (p<0.05). Spermatogonia, pachytene spermatocyte and round spermatocyte numbers decreased in all stages in both the VPA and OXC groups compared to the control group, though this was not statistically significant (p>0.05). Apoptotic cell counts and p53 immunoreaction were significantly high and TGF-β1 expression was significantly lower in the VPA group compared to that of the control group (p<0.05). In the OXC group, p53 immunoreaction and TGF-β1 expression decreased compared to the control group, but this difference did not attain statistical significance (p>0.05).nnnCONCLUSIONSnOur results show that VPA treatment from prepuberty to adulthood significantly negatively affects spermatogenesis, not only by reducing testicular weight, but also by increasing apoptotic death and p53 and decreasing TGF-β1 activation. OXC has a minimal side effect on testicular development.

ESUT expert group on laparoscopy proposes uniform terminology during radical prostatectomy: We need to speak the same language

Department of Urology, SLK Kliniken Heilbronn, University of Heidelberg, Germany; Department of Urology, AMC Amsterdam, University of Amsterdam, The Netherlands; Department of Urology, Centre of Oncology, Kielce, Poland; Department of Urology, Pansadoro Foundation, Rome, Italy; Department of Urology, Luigi Sacco Hospital, University of Milan, Italy; Department of Urology, Medical School Leipzig, University of Leipzig, Germany; Department of Urology, Institute Montsouris, Paris, France; Department of Urology, University of Patras, Greece; Department of Urology, Gazi University of Ankara, Turkey; Department of Urology, Helios Kliniken Mullheim, Germany; Department of Urology, Klinikum Winterthur, Switzerland; Department of Urology, University of Basel, Switzerland; Department of Urology, Institute Jules Bordet – Universite Libre de Bruxelles, Brussels, Belgium