M. Cathryn Ryan

Prevalence of Anaerobic Ammonium-Oxidizing Bacteria in Contaminated Groundwater

Anaerobic ammonium-oxidizing (anammox) bacteria perform an important step in the global nitrogen cycle: anaerobic oxidation of ammonium and reduction of nitrite to form dinitrogen gas (N(2)). Anammox organisms appear to be widely distributed in natural and artificial environments. However, their roles in groundwater ammonium attenuation remain unclear and only limited biomarker-based data confirmed their presence prior to this study. We used complementary molecular and isotope-based methods to assess anammox diversity and activity occurring at three ammonium-contaminated groundwater sites: quantitative PCR, denaturing gradient gel electrophoresis, sequencing of 16S rRNA genes, and (15)N-tracer incubations. Here we show that anammox performing organisms were abundant bacterial community members. Although all sites were dominated by Candidatus Brocadia-like sequences, the community at one site was particularly diverse, possessing four of five known genera of anammox bacteria. Isotope data showed that anammox produced up to 18 and 36% of N(2) at these sites. By combining molecular and isotopic results we have demonstrated the diversity, abundance, and activity of these autotrophic bacteria. Our results provide strong evidence for their important biogeochemical role in attenuating groundwater ammonium contamination.

Differential depth distribution of microbial function and putative symbionts through sediment-hosted aquifers in the deep terrestrial subsurface

An enormous diversity of previously unknown bacteria and archaea has been discovered recently, yet their functional capacities and distributions in the terrestrial subsurface remain uncertain. Here, we continually sampled a CO2-driven geyser (Colorado Plateau, Utah, USA) over its 5-day eruption cycle to test the hypothesis that stratified, sandstone-hosted aquifers sampled over three phases of the eruption cycle have microbial communities that differ both in membership and function. Genome-resolved metagenomics, single-cell genomics and geochemical analyses confirmed this hypothesis and linked microorganisms to groundwater compositions from different depths. Autotrophic Candidatus “Altiarchaeum sp.” and phylogenetically deep-branching nanoarchaea dominate the deepest groundwater. A nanoarchaeon with limited metabolic capacity is inferred to be a potential symbiont of the Ca. “Altiarchaeum”. Candidate Phyla Radiation bacteria are also present in the deepest groundwater and they are relatively abundant in water from intermediate depths. During the recovery phase of the geyser, microaerophilic Fe- and S-oxidizers have high in situ genome replication rates. Autotrophic Sulfurimonas sustained by aerobic sulfide oxidation and with the capacity for N2 fixation dominate the shallow aquifer. Overall, 104 different phylum-level lineages are present in water from these subsurface environments, with uncultivated archaea and bacteria partitioned to the deeper subsurface.Analysis of a CO2-driven geyser over a complete eruption cycle showed temporal changes in microbial community composition and function, associated with eruption phase and aquifer water depth, and revealed a putative archaeal symbiosis.

Can CO2 trigger a thermal geyser eruption

Geyser eruptions are produced by a complex and poorly understood set of subsurface processes and conditions. They typically have an abundant supply of water, relatively permeable and competent subsurface material, a conduit to the surface, a driving mechanism (commonly believed to be the initiation of gas lift pumping by steam formation in the conduit), and a trigger. Here we present time series of dissolved CO 2 concentrations in near-surface discharge waters of a thermal geyser in Yellowstone National Park (northwestern United States) that vary systematically over several eruption cycles. Chemical geothermometry, combined with a temperature profile in a nearby well, suggests that the geyser water ascends from non-boiling conditions (∼153–171 °C at a depth of 57–65 m). When the time series of near-surface measured CO 2 concentrations are extrapolated to these subsurface conditions assuming dominantly adiabatic cooling, the additional gas pressure from dissolved CO 2 is large enough to cause the total dissolved gas pressure to exceed bubbling pressure, inducing bubble formation. We postulate that CO 2 is a necessary component to triggering eruptions in the geyser studied. Furthermore, unlike steam, CO 2 bubbles do not completely re-condense during cooling in the geyser conduit, hence providing better sustenance for gas lift pumping than pure H 2 O boiling.

Use of δ 15 N and δ 18 O Values for Nitrate Source Identification under Irrigated Crops: A Cautionary Vadose Zone Tale

Source nitrogen (N) identification of leachate or groundwater nitrate is complicated by N source mixing and N and oxygen (O) isotope fractionation caused by microbial N transformations. This experiment examined the δN and δO values in leachate collected over 1 yr at 55 cm below raspberry ( L.) plots receiving either synthetic fertilizer (FT) or poultry manure (MT). The large ranges of δN (FT: -2.4 to +8.7‰, MT: +1.6 to +9.6‰) and δO (FT: -9.9 to -0.3‰, MT: -10.9 to +1.7‰) values in leachate collected under crop rows prohibited the reliable identification of the applied N sources on individual sampling dates. However, the mass-weighted average δN (FT: +3.2‰, MT: +7.3‰) values in leachate were significantly different and can be explained by accounting for the estimated contributions of nitrate and δN values of the various N sources, including applied fertilizer (-0.7‰) or manure (+7.9‰), nitrate-rich irrigation water (+9.0‰), and nitrate from soil N mineralization and nitrification (FT: +3.7‰, MT: +4.6‰; the seasonal timing of which is unknown). This study illustrates the importance of characterizing all major N sources and considering the seasonal variation of these sources and of N cycling processes, as they contribute to the δN values of leachate.

Relative importance of P and N in macrophyte and epilithic algae biomass in a wastewater-impacted oligotrophic river.

The role of nutrient loading on biomass growth in wastewater-impacted rivers is important in order to effectively optimize wastewater treatment to avoid excessive biomass growth in the receiving water body. This paper directly relates wastewater treatment plant (WWTP) effluent nutrients (including ammonia (NH3-N), nitrate (NO3-N) and total phosphorus (TP)) to the temporal and spatial distribution of epilithic algae and macrophyte biomass in an oligotrophic river. Annual macrophyte biomass, epilithic algae data and WWTP effluent nutrient data from 1980 to 2012 were statistically analysed. Because discharge can affect aquatic biomass growth, locally weighted scatterplot smoothing (LOWESS) was used to remove the influence of river discharge from the aquatic biomass (macrophytes and algae) data before further analysis was conducted. The results from LOWESS indicated that aquatic biomass did not increase beyond site-specific threshold discharge values in the river. The LOWESS-estimated biomass residuals showed a variable response to different nutrients. Macrophyte biomass residuals showed a decreasing trend concurrent with enhanced nutrient removal at the WWTP and decreased effluent P loading, whereas epilithic algae biomass residuals showed greater response to enhanced N removal. Correlation analysis between effluent nutrient concentrations and the biomass residuals (both epilithic algae and macrophytes) suggested that aquatic biomass is nitrogen limited, especially by NH3-N, at most sampling sites. The response of aquatic biomass residuals to effluent nutrient concentrations did not change with increasing distance to the WWTP but was different for P and N, allowing for additional conclusions about nutrient limitation in specific river reaches. The data further showed that the mixing process between the effluent and the river has an influence on the spatial distribution of biomass growth.

Changes in Water Quality Characteristics and Pollutant Sources Along a Major River Basin in Canada

Temporal and spatial variations of water quality along the Bow River (Alberta, Canada) were investigated using monthly water quality data (chloride, sulphate, nitrate, sodium, and conductivity) collected from 2004 to 2011. Non-point and point (notably three wastewater treatment plants) pollutant loads were characterized along the river. The river was divided into three reaches, namely, the Upper river reach, the Calgary reach, and the Downstream river reach, based on the distribution of point pollutant sources and geographic conditions. A mass balance approach and statistical analyses were employed to analyze water quality. The results demonstrated that the point sources, Calgary’s three wastewater treatment plants (WWTPs), are largely responsible for the observed spatial and temporal trends in the investigated quality parameters. However, the contribution of non-point sources appears to vary along the river, which might be related to the flow pathways taken by non-point pollutants discharging into the river and the geochemical characteristics of the groundwater within the alluvial aquifer that is hydraulically connected to the river. Apart from the identified point and non-point sources, the effects of other processes such as biological reactions need to be further ascertained and quantified for a better assessment of pollutant loads, in particular nutrients. Further understanding of these issues will allow a more accurate quantification of pollutant loads and consequently, better knowledge for formulating reliable water quality management strategies.

Nuclear Waste Disposal: A Cautionary Tale for Shale Gas Development

Nuclear energy and shale gas development each began with the promise of cheap, abundant energy and prospects for national energy independence. Nuclear energy was touted as “too cheap to meter,” and shale gas promised jobs and other economic benefits during a recession.

A Field-Scale Approach to Estimate Nitrate Loading to Groundwater

The quantification of groundwater NO loading associated with a specific field or set of management practices so that groundwater quality improvements can be objectively assessed is a major challenge. The magnitude and timing of NO export from a single agricultural field under raspberry ( L.) production were investigated by combining high-resolution groundwater NO concentration profiles (sampled using passive diffusion samplers) with Darcys flux estimation at the fields down-gradient edge (based on field-measured hydraulic gradients and laboratory-estimated hydraulic conductivity). Annual recharge estimated using Darcys law (1002 mm) was similar to that obtained using two other approaches. The similarity in the rate of Cl applied to the field and the estimated export flux over the 1-yr monitoring period (51 vs. 56 kg Cl ha) suggested the mass flux estimation approach was robust. An estimated 80 kg NO-N ha was exported from the agricultural field over the 1-yr monitoring period. The greatest monthly groundwater mass flux exported was observed in February and March (∼11 kg NO-N ha), and was associated with NO leached from the soil zone during the onset of precipitation in the previous autumn. Provided the groundwater recharged from the field of interest can be isolated within a vertical profile, this approach is an effective method for obtaining spatially integrated estimates of the magnitude and timing of NO loading to groundwater.

Groundwater contribution keeps trophic status low in Sylvan Lake, Alberta, Canada

Water well drilling records combined with geologic observation around Sylvan Lake suggest a permeable, fractured groundwater-bearing channel sandstone extends along the northeast margin of the lake, and is surrounded by a matrix of shale with small sandy interbeds. Chloride and water isotope mass-balance approaches found groundwater fluxes were 37 to 43% of total annual lake water inputs, and were consistent with Darcy flux estimates for the channel sandstone. The groundwater contribution to the lake water budget contributes to an estimated lake-water residence time ranging from 22 to 30 years, which is much lower than other lakes in the region and lakes of similar size. Groundwater through-flow in the hydraulically connected channel sandstone unit of the Paskapoo Formation appears to play a critical role in maintaining the relatively low total dissolved solids (and trophic status) of Sylvan Lake, as compared to other lakes in south-central Alberta. Due to the hydraulic connection of the sandstone channel with Sylvan Lake, land use along the north side of the lake (above the sandstone channel unit) should be carefully considered to mitigate groundwater (and consequently lake water) quality impacts.