M Eugene

Beneficial effects of a low-potassium+ and polyethylene glycol solution on renal function and structure during 48-hour cold storage preservation

Abstract ALTHOUGH the success of the first cold storage solution was attributed to its intracellular composition with low sodium and high potassium,1,2 the importance of impermeant agents has been shown and the potential deleterious effects of high potassium solutions have been suggested.3–5

Ischemia-reperfusion injury is associated with inflammatory cell infiltration: evaluation in a pig kidney autotransplant model.

DAMAGE sustained by the allograft during the process of transplantation has been shown to be influenced by cold ischemia-reperfusion injury, which is one of the principal “antigen-independent” factors. A previous study demonstrated that tissue injury occurs within isografts as compared to allografts, in the absence of alloantigen stimulation. In a rat model, a recent report suggests that molecules involved in T-cell costimulation may play a key role in ischemia-reperfusion in the kidney, independent of any alloimmune stimuli. The aim of this study was to assess the effect of the length of cold ischemic time in an autotransplant pig kidney model on delayed graft function and inflammatory cell infiltration.

Proton NMR spectroscopy as a novel approach to the monitoring of citrate and trimethylamine-N-oxide excretion after kidney preservation

Abstract HIGH resolution protin nuclear magnetic resonance (NMR) spectroscopy can be used for the rapid multicomponent analysis of low molecular weight metabolites in biologic fluids such as plasma and urine. Citrate (Cit) is an organic acid normally found in human urine. Reduced urinary Cit excretion has been described in HgCl 2 and p -aminophenol intoxication. 1 The mechanism is impairment of the Krebs cycle. Trimethylamine-N-oxide (TMAO) is an osmolyte molecule synthesized in the medullar cells of the kidney. 2 In plasma and urine, TMAO is not usually detected. Its excretion may be related to leakage from the renal medulla cells after intense medullar ischemia. The aim of this study was to assess Cit and TMAO excretion after prolonged cold storage (CS) with two standard solutions. These markers are precisely detected by proton NMR spectroscopy.

Evaluation of renal medulla injury after cold preservation and transplantation: noninvasive determination of medullar damage by proton nuclear magnetic resonance spectroscopy of urine and plasma ☆

PRIMARY and early allograft dysfunction remains a challenge for the nephrologist. Usually, the assessment of renal graft dysfunction following transplantation is based on nonspecific measurement of renal function or an invasive method such as transplant biopsy. A rapid diagnosis is necessary because a delay in treating early rejection deteriorates the prognosis of the allograft function. In addition, the antirejection treatment based on a mere clinical diagnosis of rejection could improve delayed graft function caused by acute tubular necrosis or cyclosporine damage through possible side effects of antirejection treatment. In the present study, we wanted to assess whether proton nuclear magnetic resonance (HNMR) spectroscopy can detect ischemic damage and predict acute tubular necrosis after pig kidney preservation in two preservation solutions (Euro-Collins: EC and University of Wisconsin: UW).

Comparison of Euro-Collins and university of wisconsin solutions in the isolated perfused rat liver model: evaluation by proton nuclear magnetic resonance spectroscopy.

PRIMARY dysfunction or nonfunction of liver graft is still an important problem in liver transplantation despite surgical progress. The determination of new markers of graft viability and metabolic activities seems to be an important challenge to assess the early graft function. Proton nuclear magnetic resonance spectroscopy (NMRp) has previously demonstrated its interest in the evaluation of renal allograft dysfunction after ischemia reperfusion in isolated perfused pig kidney. For the kidney, NMRp study determines specific markers of function like TMAO and citrate. The aim of this study was to assess liver graft function in an isolated perfused rat liver model after 24 hours of preservation in Euro-Collins (EC) or University of Wisconsin (UW) solution.