M. Giammarco

Effects of selenium source and level of supplementation on the performance and meat quality of lambs.

Objective of this study was to evaluate the performance, the quality and oxidative stability of meat, the total Se and specific selenoamino-acids content of muscle of lambs that were fed diets supplemented from different Se sources and at different levels. Forty-eight Apennine lambs 30day old (12.78±0.94kg) received, during a 63day period, a total mixed ration (TMR) which was either Se unsupplemented (Control group - background only- 0.13mg/kg Se) or supplemented with Na selenite (0.30mg/kg Se as sodium selenite) or selenium enriched yeast (0.30mg/kg and 0.45mg/kg Se as Se-yeast). Growth performance, feed to gain ratio, carcass and meat quality (pH, drip and cooking losses, colour, GSH-Px activity and chemical analysis) did not show any difference between the treatments. Meat colour and oxidative stability during 9 days of refrigerated storage were unaffected by dietary supplementation, suggesting that, at the levels of Se used in this experiment, dietary Se, even from an organic source, had limited potential for reducing lipid oxidation. Selenium supplementation raised the Se content in muscle (P<0.001) with the greatest increase when Se-yeast was fed. Although selenite increased total Se, it did not influence total or specific selenoamino-acids in this tissue. On the contrary, Se-yeast supplementation led to an increase in muscle Se-methionine content. We conclude that Se supplementation can increase significantly muscle Se levels and produce, particularly when Se-yeast is fed, a source of Se enriched meat as Se-methionine.

Effects of loading methods on rabbit welfare and meat quality

The effects of different loading methods on the welfare, carcass characteristics and meat quality traits of hybrid commercial rabbits were investigated. 384 male rabbits, 82 days old, were transported from the farm to the slaughterhouse. At the farm, 192 rabbits were loaded onto the truck smoothly (S) and 192 rabbits were loaded roughly (R). The S loading method consisted of carefully placing each rabbit into the transport crates. In the R method, the loading was hurriedly and carelessly executed by the transport operator, throwing each animal into the crates fixed on the truck. Live weight before and after transport as well as slaughter data were recorded for each rabbit, and a subset of 80 carcasses were evaluated for meat quality. Blood samples from 80 rabbits were analysed for haematological and biochemical parameters. A significant neutrophilia (P<0.001), lymphocytopaenia (P<0.001) and an increase in serum aspartate aminotransferase (AST) (P<0.01), alanine aminotransferase (ALT) (P<0.001) and creatine kinase (CK) activities (P<0.001) were recorded in all rabbits after transport, independent of the loading method. A twofold increase in serum corticosterone concentration (6.23 vs. 14.88 ng/mL; P=0.001) was observed in all rabbits following transport. Results suggest that the stress parameters analysed were more influenced by transport and handling itself rather than by the different loading methods. The results showed that there was no adverse effect of loading method on carcass traits. Furthermore, the stress condition evidenced by haematological and biochemical parameters prior to slaughter did not affect meat quality.

Effects of the rearing season on carcass and meat quality of suckling Apennine light lambs.

The aim of this study was to evaluate the effects of the rearing season (autumn vs winter) on the carcass and meat quality of light lambs, obtained according to the traditional farming system usual in central Italy. Eighty carcasses from 60 d+/-3 old unweaned Apennine single birth male lambs, permanently reared indoor, half in autumn (receiving milk from ewes permanently pastured) and half in winter (which dams did never acceded to pasture), were weighed, classified according to the EU classification system for light lambs, and their Longissimus lumborum meat was analysed for pH, colour, drip and cooking losses, proximate composition and fatty acids profile of intramuscular fat. Most of the carcasses fully responded to the 1st quality EU requirements and no effects of the rearing season was evident on carcass characteristics. On the contrary, L. lumborum of lambs born and reared in autumn, receiving milk from ewes permanently pastured, evidenced a lower Lightness L* (P=0.02), a higher Chroma C* (P=0.01), with a higher fat content (P=0.04) than lambs reared in winter, which dams were permanently stall-fed. Moreover the intramuscular fat of the former was characterized by a greater PUFA concentration (P=0.01), a more favourable n-6/n-3 ratio (P<0.001), and a higher CLA content (P<0.001) than the latter, as a result of the difference related to the sheep traditional feeding system.

Effects of extruded linseed dietary supplementation on milk yield, milk quality and lipid metabolism of dairy cows

Abstract Twenty Italian Friesian dairy cows were used in an experimental trial to study the effects of extruded linseed dietary supplementation on milk production, milk quality and fatty acid (FA) percentages of milk fat and total plasma lipids and plasma phospholipids. Control cows were fed a corn silage based total mixed ration (TMR) while treated animals also received 700g/head/d of extruded linseed supplementation. Feed intake was similar between groups. Milk yields was tendentially greater for cows fed extruded linseed. Milk urea content (P<0.05) were reduced by treatment. Results showed a significant increase n-3 FA concentration (particularly alpha linolenic acid) and a significant reduction of n-6/n-3 FA ratio in milk fat, total plasma lipids and plasma phospholipids (P<0.001); moreover a reduction trend (P<0.1) of arachidonic acid concentrations was observed in milk fat, total plasma lipids and plasma phospholipids. At last, treatment enhanced milk fat conjugated linoleic acid (CLA) percentage (P<0.05).

Effects of Se supplementation on growth rate and blood parameters in lambs

Abstract Forty-eight Appenninica lambs 30 d old received during a 63 d period the same diet (dry unifeed) supplied 4 different mineral premixes differing for their Se sources and levels to obtain the following 4 treatments (on an as fed basis): Control group (T1) - 0mg/kg Se; T2 – 0.30mg/kg Se as sodium selenite; T3 – 0.30mg/kg and T4 – 0.45mg/kg Se as Se yeast (Saccharomyces cerevisiae). Biweekly all the animals were weighed and feed consumption recorded. Moreover, at start (t0) and successively each two weeks (t14, t28, t42, t56), all the animals were blood sampled in order to evaluate plasma and whole blood Se concentration and blood glutathione peroxidase (GSH-Px) and creatine phosphokinase (CPK) activities. Daily weight gain (averaging 166±6g/d) never differed between treatments. Se supplementation increased Se concentration in blood in relation to dietary level (p<0.05) and, compared to selenite, Se yeast seemed more effective to obtain higher concentration in whole blood (P<0.05) but not in plasma. Blood GSH-Px activity was higher in lambs receiving Se supplementation but was not influenced by Se dietary level or source. No effects could be find on blood CPK activity.

Feeding dehydrated alfalfa increases polyunsaturated fatty acids concentration in Marchigiana beef muscle

Riassunto L’alimentazione con erba medica disidratata eleva il contenuto di acidi grassi polinsaturi delle carni di vitelloni di razza Marchigiana. La ricerca è stata eseguita su 20 vitelloni di razza Marchigiana dai 18 ai 21 mesi. Gli animali sono stati distinti in 2 gruppi sperimentali (controllo e trattato) di 10 capi ciascuno ed alimentati con paglia, fieno e mangime fornito a volontà. I vitelloni del gruppo trattato hanno ricevuto un mangime contenente il 20% di medica disidratata mentre quelli del controllo hanno assunto un mangime di analoghe caratteristiche analitiche ma sprovvisto di foraggi disidratati. I risultati mostrano come l’alimentazione con erba medica disidratata innalzi significativamente il contenuto di acidi grassi polinsaturi (P<0,05) ed in particolare di acido linoleico (C18:3n-3; P<0,001) ed arachidonico (C20:4n-6; P<0,01).

Analgesic effects of intravenous flunixin and intrafunicular lidocaine or their combination for castration of lambs

Objective To analyse the effectiveness of intrafunicular lidocaine and intravenous flunixin for reducing pain and signs of stress in lambs undergoing surgical castration. Design Randomised controlled trial. Setting One university teaching hospital in Italy. Participants 30 healthy male lambs, 9–12 weeks old. Intervention Allocation to five groups: a control group (C), undergoing general anaesthesia but not castration; a surgery group (S), undergoing orchiectomy without analgesic treatment; a surgery-lidocaine group (SL), undergoing orchiectomy and receiving intrafunicular 2 per cent lidocaine solution; a surgery-flunixin group (SF), undergoing orchiectomy and receiving intravenous flunixin; a surgery-flunixin-lidocaine group (SFL), undergoing orchiectomy and receiving both intrafunicular lidocaine and intravenous flunixin. Main outcome measures Nociception and stress were assessed through intraoperative indicators, serum cortisol concentration, glycaemia, behaviour, immune response and clinical evaluation of the heart rate (HR), respiratory rate and rectal temperature after surgery. Results Groups S and SL showed increased values of intraoperative HR, mean arterial pressure and postoperative cortisol concentration. In group SFL, cortisol values were similar to those of group C. No other difference could be detected. Conclusions The combination of intravenous flunixin and intrafunicular lidocaine reduced the pain and discomfort of lambs castrated under general anaesthesia. Intrafunicular lidocaine alone did not prevent pain or discomfort associated with castration. Trial registration number 30/2012/CEISA/COM.

Effects of a single injection of Flunixin meglumine or Carprofen postpartum on haematological parameters, productive performance and fertility of dairy cattle

The objective of the present research was to evaluate the effects of a single injection of Flunixin meglumine (FM) or Carprofen postpartum on haematological indicators, productive performance and fertility of Italian Friesian dairy cattle. In total, 60 cows, including 42 multiparous and 18 primiparous, were assigned to one of the following three treatments: (1) FM (2.2 mg i.m./kg of bodyweight (BW); Meflosyl 50 mg/mL), (2) Carprofen (CA; 1.4 mg s.c./kg of BW; Rymadil 50 mg/mL) or (3) saline (control) at 2.0 mL s.c./45.5 kg of BW. All treatments were administrated by a single injection within 12 h after calving. Individual milk yield was daily recorded during the trial and composition was determined at 7, 14, 21, 28, 35 and 60 days in milk (DIM). BWs were recorded at –21 ± 5 days before calving, and 1, 7, 14, 21, 28, 35 and 60 days after calving before the morning milking. Individual blood samples were collected from each animal for haemato-biochemical evaluation 3 weeks before calving (T0) and then repeated at the following times: within 12 h after calving, immediately before the administration of the anti-inflammatory drug (FM or CA; T1), ~36 h after calving (T2), 4 days after calving (T3) and 11 days after calving (T4). At each sampling time, the rectal temperature and the heart rate were monitored. Body condition score was determined after each sampling time and at 35 and 60 DIM. FM and CA treatments did not influence rectal temperature and heart rate during the first 11 DIM; no differences in overall milk yield, milk composition and dry matter intake were found. BW and body condition score were not affected by treatments throughout the study. Treatments did not affect serum metabolite concentrations of glucose, non-esterified fatty acids, β-hydroxybutyrate, serum urea nitrogen, total protein and albumin. Control cows showed a higher culling rate (P < 0.05) than did the FM- and CA-treated cows (25% vs 15% vs 5% respectively). FM-treated multiparous cows had a significantly (P < 0.05) lower incidence of retained placenta than did control and CA-treated cows (2.3% vs 9.3% vs 14% respectively). Furthermore, a greater percentage of cows pregnant (35% vs 10%) at the first insemination (P < 0.001) in non-steroidal anti-inflammatory drug groups than in control was found. Our findings evidenced that a single injection of FM or CA to non-febrile cows immediately after parturition could positively affect the metabolic adaptation of the cows at the onset of lactation and this aspect can positively influence reproductive performances and the culling rate.