M. Sierra

Determination of passive viscoelastic response of the abdominal muscle and related constitutive modeling: Stress-relaxation behavior

In this paper, the authors investigate the passive viscoelastic properties of rabbit abdominal wall. In vitro strain relaxation tests were performed in the oblique muscle (in two perpendicular directions), the rectus abdominis and the linea alba in the longitudinal direction. Based on experimental data, a model for the viscoelastic mechanical properties of this tissue is presented here. In particular, we used a 3D non-linear viscoelastic model to fit data sets obtained from tissue of the rabbit abdominal wall. Uniaxial relaxation tests were carried out for samples obtained from the abdominal wall. The experimental results clearly demonstrate the anisotropy and nonlinearity of the abdominal tissue. The stress relaxation was higher in the transverse direction (closer to muscle fibers) with an average value of the final stress ratio of 48%, than in the longitudinal direction with around 56% of this ratio for the oblique muscle. These tests, at several stretch levels, presented a different behavior depending on the region where the tissue sample was located. There was no dependence between the stress relaxation ratio and the stretch level for the oblique muscles in their longitudinal or transverse directions (p>0.01). In contrast, for rectus abdominis and linea alba a dependence between the stress relaxation ratio and stretch level was found. Our study revealed an increase in the stress relaxation ratio for the rectus abdominis (p<0.01) and a decrease for the linea alba with higher stretch levels (p<0.01). Overall good predictions ε<0.115 were obtained with the model proposed for the oblique muscle (no dependence on the stretch level) and to reproduce the non-linear viscoelastic response of rectus abdominis and linea alba.

Active behavior of abdominal wall muscles: Experimental results and numerical model formulation

In the present study a computational finite element technique is proposed to simulate the mechanical response of muscles in the abdominal wall. This technique considers the active behavior of the tissue taking into account both collagen and muscle fiber directions. In an attempt to obtain the computational response as close as possible to real muscles, the parameters needed to adjust the mathematical formulation were determined from in vitro experimental tests. Experiments were conducted on male New Zealand White rabbits (2047±34g) and the active properties of three different muscles: Rectus Abdominis, External Oblique and multi-layered samples formed by three muscles (External Oblique, Internal Oblique, and Transversus Abdominis) were characterized. The parameters obtained for each muscle were incorporated into a finite strain formulation to simulate active behavior of muscles incorporating the anisotropy of the tissue. The results show the potential of the model to predict the anisotropic behavior of the tissue associated to fibers and how this influences on the strain, stress and generated force during an isometric contraction.

Predicting muscle fatigue: a response surface approximation based on proper generalized decomposition technique

A novel technique is proposed to predict force reduction in skeletal muscle due to fatigue under the influence of electrical stimulus parameters and muscle physiological characteristics. Twelve New Zealand white rabbits were divided in four groups (

Effect of Cryopreserved Amniotic Membrane on the Mechanical Properties of Skeletal Muscle after Strabismus Surgery in Rabbits

n=3

On Using Model Populations to Determine Mechanical Properties of Skeletal Muscle. Application to Concentric Contraction Simulation

n=3) to obtain the active force evolution of in vitro Extensor Digitorum Longus muscles for an hour of repeated contractions under different electrical stimulation patterns. Left and right muscles were tested, and a total of 24 samples were used to construct a response surface based in the proper generalized decomposition. After the response surface development, one additional rabbit was used to check the predictive potential of the technique. This multidimensional surface takes into account not only the decay of the maximum repeated peak force, but also the shape evolution of each contraction, muscle weight, electrical input signal and stimulation protocol. This new approach of the fatigue simulation challenge allows to predict, inside the multispace surface generated, the muscle response considering other stimulation patterns, different tissue weight, etc.

腹壁筋の活性挙動:実験結果と数値モデルの定式化【Powered by NICT】

A response surface was built to predict the lipid peroxidation level, generated in an iron-ascorbate in vitro model, of any organ, which is correlated with the oxidative stress injury in biological membranes. Oxidative stress studies are numerous, usually performed on laboratory animals. However, ethical concerns require validated methods to reduce the use of laboratory animals. The response surface described here is a validated method to replace animals. Tissue samples of rabbit liver, kidney, heart, skeletal muscle and brain were oxidized with different concentrations of FeCl3 (0.1 to 8mM) and ascorbate (0.1mM), during different periods of time (0 to 90min) at 37°C. Experimental data obtained, with lipid content and antioxidant activity of each organ, allowed constructing a multidimensional surface capable of predicting, by interpolation, the lipid peroxidation level of any organ defined by its antioxidant activity and fat content, when exposed to different oxidant conditions. To check the predictive potential of the technique, two more experiments were carried out. First, in vitro oxidation data from lung tissue were collected. Second, the antioxidant capacity of kidney homogenates was modified by adding melatonin. Then, the response surface generated could predict lipid peroxidation levels produced in these new situations. The potential of this technique could be reinforced using collaborative databases to reduce the number of animals in experimental procedures.

Caracterización de las propiedades mecánicas del tejido muscular. Una aproximación numérica utilizando poblaciones de modelos

ABSTRACT Purpose: To study the functional recovery of the superior rectus muscle (SRM) after its partial resection in a rabbit model with and without cryopreserved amniotic membrane (AM). Material and methods: Resection of the right and left SRMs of 30 rabbits was performed. On the left eyes, a single sheet of equine cryopreserved AM was placed covering the muscle edge sutured. Active and passive mechanical properties of muscles operated with and without AM were monitored over time at 30 (n = 10), 60 (n = 10), and 90 (n = 10) days after surgery. Muscle samples were extracted and electrically stimulated to register the force exerted by the samples, characterizing its active behavior. They were, then, subjected to stretching test to obtain its resistance to deformation, known as passive behavior. Moreover, right and left eyes of a control group (n = 5) were equally subjected to active and passive tests to characterize the physiological behavior of SRM muscles. Results: On active function examination, statistically significant differences were documented between the following: control vs AM and no AM at 30 days (p = 0.002 and p = 0.04, respectively). All other comparisons were insignificant (p > 0.05). On passive function analysis, significant differences were only found between control vs. no AM at 30 days (p = 0.004) and between AM vs. no AM at 30 days (p = 0.002). Indeed, muscle operated without AM did not recover a normal passive function until 60 days after surgery. Conclusion: Cryopreserved AM is effective in accelerating recovery of SRM passive function in rabbits. Nevertheless, AM produced no significant effect on recovery of SRM active function.