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Featured researches published by M. Y. Hasegawa.


African Journal of Traditional, Complementary and Alternative Medicines | 2016

Anthelmintic effects of dried ground banana plant leaves ( Musa spp.) fed to sheep artificially infected with Haemonchus contortus and Trichostrongylus colubriformis

Lilian Gregory; Eidi Yoshihara; Leandro Kataoaka Fernandes Silva; Eduardo Carvalho Marques; Bruno Leonardo Mendonça Ribeiro; Enoch Brandão de Souza Meira Júnior; Rodolfo Silva Rossi; Alessandro Francisco Talamini do Amarante; M. Y. Hasegawa

Background: Helminths is a endoparasites that cause the major losses for profitable sheep production in Brazil. The increased development of resistant strains of endoparasites have enforced the search for sustainable alternatives. The aim of this paper was to provide information about endoparasites control with banana leaves in infected sheep as alternative control strategies and see its viability. Materials and Methods: In this study, we performed two trials to investigate the anthelmintic properties of banana leaves on endoparasites in sheep. In Trial 1, twelve sheep were artificially infected with Trichostrongylus colubriformis; in Trial 2, eleven sheep were artificially infected with Haemonchus contortus. Clinical examinations, packed cell volume, total protein, faecal egg counts (FECs) and egg hatchability tests (EHTs) were performed. At the end of the trials, the sheep were humanely slaughtered, and total worm counts were performed. Results: In Trial 1 and 2, no significant FEC decreases were note but significant diference in EHTs were observed. Total worm counts, clinical and haematological parameters did not reveal significant changes between the treatment and control groups. These results suggest that feeding dried ground banana plant leaves to sheep may reduce the viability of Trichostrongylus colubriformis eggs, and this anthelmintic activity is potentially exploitable as part of an integrated parasite management programme. Conclusion: However, further investigation is needed to establish the optimal dosage, develop a convenient delivery form and confirm the economic feasibility of using banana plantation byproducts as feed for ruminant species. Abbreviations: Coproculture test (CT)., Faecal egg count (FEC)., Egg hatchability test (EHT)


Arquivos do Instituto Biológico | 2013

Pesquisa de anticorpos contra Maedi-Visna em ovinos nas microrregiões de Botucatu, Campinas, Piedade e São Paulo, Estado de São Paulo

Lilian Gregory; Maria do Carmo Custódio de Souza Hunold Lara; A.C.M. Kiraly; M. Y. Hasegawa; H. Rizzo; L.C.S. Henriques; R.S. Rossi; Roberto Soares de Castro

The purpose of the study was to evaluate the occurrence of infection with maedi-visna virus in sheep raised in the regions of Botucatu, Campinas, Piedade and Sao Paulo, state of Sao Paulo, Brazil, that showed symptoms of the disease. Blood serum samples collected from 226 sheep were submitted to the agar gel immunodiffusion technique for detection of antibodies against maedi-visna virus, and none of the samples tested was serum reactive. In conclusion, the maedi-visna virus has low frequency in animals raised in the regions studied.


Pesquisa Veterinaria Brasileira | 2016

Avaliação dos fatores etários sobre a função renal de ovelhas Santa Inês

Laura Cristina Sant‟Anna Henriques; Lilian Gregory; Huber Rizzo; M. Y. Hasegawa; Enoch Brandão de Souza Meira

This study evaluated kidney function of healthy Santa Ines ewes, bred in Sao Paulo State. The aim of this paper was to establish standard reference values and stipulate value variation caused by age group factor. We determined seric and urinary levels of urea, creatinine, sodium and potassium, and urinary GGT from 128 ewes, divided into 4 groups according to age. Also urinalysis was made in 128 urine samples. No significant statistical difference was found regarding density values between all groups, although there was an increase in urea values, creatinine and sodium, and an decrease in urinary proteins and GGT in group 2, 3 and 4 older than group1. In relation to the seric parameters, there was significative statistical difference in urea and creatinine values between the groups, as an increase in urea and creatinine. Potassium decreased in the older groups 2, 3 and 4. Evaluating the results obtained for renal function, we found that the mean values of density were 1.03±0.01, pH 7.1±1.35, urea (mg/dL) 1342±1085, creatinin 42.39±23,36, total protein (g/dL) 5.61±2.88, GGT (U/L) 1.44±1.31, sodium and potassium (mmol/L) were 15.93±20.13 and 240±117 respectively. In the serum medium values were: urea (mg/dL) 32.33±19.81, creatinine 1.22±0.20, sodium and potassium (mmol/L) 145.7±8.8 and 5.5±1.30 respectively.


Ars Veterinaria | 2009

Detecção do virus da artrite encefalite caprina em Amostras de leite de cabras pela reação em cadeia Da polimerase (pcr) e nested-pcr

Lilian Gregory; M. C. C. S. H. Lara; E. M. C. Villalobos; M. Y. Hasegawa; J.N.M. Rodrigues; Jansen de Araujo; L. W. Keller; Edison Luiz Durigon


Parasitology Research | 2015

Dried, ground banana plant leaves (Musa spp.) for the control of Haemonchus contortus and Trichostrongylus colubriformis infections in sheep.

Lilian Gregory; E. Yoshihara; Bruno Leonardo Mendonça Ribeiro; L. K. F. Silva; Eduardo Marques; E. B. S. Meira; R. S. Rossi; P. H. Sampaio; H. Louvandini; M. Y. Hasegawa


Arquivos do Instituto Biológico | 2011

Detecção do vírus da artrite encefalite caprina no sêmen através das técnicas de PCR e NESTED-PCR

Lilian Gregory; Maria do Carmo Custódio de Souza Hunold Lara; M. Y. Hasegawa; J.N.M. Rodrigues; Jansen de Araujo; L. W. Keller; L. K.F. Silva; Edison Luiz Durigon


Acta Scientiae Veterinariae | 2018

Studying the elimination pattern of Caprine Arthritis Encephalitis Virus in the milk of infected females

Natália Carrillo Gaeta; M. Y. Hasegawa; Bruno Leonardo Mendonça Ribeiro; Ana Lisa do Vale Gomes; Roberto Soares de Castro; Lilian Gregory


Small Ruminant Research | 2016

Small ruminant lentivirus variants and related clinical features in goats from southeastern Brazil

M. Y. Hasegawa; E.B.S. Meira; Maria do Carmo Custódio de Souza Hunold Lara; Roberto Soares de Castro; J.N.M. Rodrigues; J. Araújo; L. W. Keller; Paulo Eduardo Brandão; H. Rizzo; M.L. Barbosa; Natália Carrillo Gaeta; R.S. Rossi; E.L. Durigon; Lilian Gregory


Small Ruminant Research | 2017

An experimental study on the vertical transmission of caprine arthritis-encephalitis virus from naturally infected females to their offspring

M. Y. Hasegawa; Maria do Carmo Custódio de Souza Hunold Lara; Eliana Monteforte Cassaro Villa Lobos; Natália Carrillo Gaeta; Mika Hayashi; Laíz Shirayama; Roberto Soares de Castro; Lilian Gregory


Pesquisa Veterinaria Brasileira | 2017

Transmissibilidade de Lentivírus de Pequenos Ruminantes para cabritos e cabras adultas por meio de sêmen infectado experimentalmente

M. Y. Hasegawa; Maria do Carmo Custódio de Souza Hunold Lara; Natália Carrillo Gaeta; Júlia Avansi Marques; Bruno Leonardo Mendonça Ribeiro; Rodolfo Santos Rossi; Eduardo Marques; Lilian Gregory

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Lilian Gregory

University of São Paulo

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Roberto Soares de Castro

Universidade Federal Rural de Pernambuco

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L. W. Keller

University of São Paulo

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