Manuel A Moreno

Discrimination index of Y-chromosomal haplotypes in an Antioquia (Colombia) population sample

Abstract Y-chromosome STR haplotypes (DYS19, DYS385, DYS389I, DYS389II, DYS390, DYS391, DYS392 and DYS393) in a sample of 507 healthy male blood donors from Antioquia (Colombia) were studied. 323 different haplotypes were found, 256 of them being unique. Gene diversity ranged between 0.3640 at DYS393 and 0.8900 at DYS385. The haplotype diversity value (power of discrimination (PD) and power of exclusion (PE)) calculated from all eight loci combined was 0.9946, which is informative. This study provides further information on the Y-chromosome polymorphisms commonly used in paternity testing, forensic genetics and in the population genetic studies of complex patterns of sex differential racial admixture.

Multiple sclerosis: association to HLA DQalpha in a tropical population.

Studies performed in subtropical populations have found significant association between the phenotype multiple sclerosis (MS) and the major histocompatibility complex (MHC). We present the results of a case-control study conducted on a tropical population (Antioquia, Colombia) in order to detect a possible association between MS and HLA DQα (HLA DQA1*) alleles. Forty chromosomes belonging to MS patients were compared to two sets of controls (40 and 910 chromosomes, respectively). The HLA DQA1*0101 and DQA1*0102 alleles were found in a significantly higher proportion among the cases than among the controls, whereas the HLA DQA1*0103 allele was found in a significantly lower proportion of the cases. These results suggest that the association of HLA DQA1*0101, DQA1*0102 and DQA1*0103 to the MS phenotype found in Caucasian subtropical populations remains in individuals with MS inhabiting the tropics. This finding could mean that the major genetic component associated to the MHC in subtropical populations is the same in the tropics.

Population genetics of eight new Y-chromosomal STR haplotypes in three Colombian populations: Antioquia, Chocó and Cartagena

Abstract In this study, eight new Y-chromosomal (GATA-C4, DYS438, DYS437, DYS461, GATA-H4, DYS439, GATA-A10 and DYS460) short tandem repeat (STR) polymorphic systems were typed by two PCR quadruplex reactions in three Colombian populations: Antioquia, Choco and Cartagena. GATA-C4 showed the highest gene diversity in the three populations. In the Antioquia population sample, a total of 123 different haplotypes were observed among which 85 were unique and 38 were found at least two times; in the Choco population sample: 55 different haplotypes, 47 unique and 8 at least two times; in the Cartagena population sample: 82 different haplotypes, 77 unique and 5 at least two times. The highest average gene diversity was found in Cartagena and the lowest was in Choco. The haplotype diversity for the eight Y-chromosomal STR loci in the Antioquia, Choco and Cartagena population samples were calculated to be 0.9949, 0.9959 and 0.9940, respectively.

Allele Frequency Distribution of Five X-Chromosomal STR Loci in an Antioquian Population Sample (Colombia)

Blood samples from unrelated Caucasian individuals from Antioquia (Colombia) were collected. DNA was extracted from 200 µL of peripherical blood by the salting-out procedure (1). The primer sequences of loci and cycling conditions were as recommended in previous studies (2–4) o from Genome Database (http://www.gdb.org). The PCR products were analyzed using denaturing 4% acrylamidebis-acrilamide gel electrophoresis and detected by silver staining. Alleles were identified based on the number of repeats and their attribution was made by comparison with an in-house constructed allelic ladder and following the published nomenclature (2–4) and ISFG guidelines for STR analysis (5).

Genetic analysis of the short tandem repeat loci FGA, D7S820, D1S355, D9S304, D12S1090, D3S1744 and D18S849 in an Antioquia (Colombia) population sample

Abstract In this study, the validation of seven short tandem repeated (STR) systems is presented in a sample of the population from Antioquia, central region of Colombia. The DNA for the analyses of PCR was obtained from 261–450 unrelated individuals (selected using some cases of paternity from the Human Genetics Laboratory, University of Antioquia, and Laboratorio de Genetica Forense y Huellas Digitales del DNA, GENES). PCR amplification was performed according to the manufacturers instructions, using the STR Multiplex I (FGA, D7S820, D1S355 and D9S304) and STR Multiplex II (D12S1090, D3S1744 and D18S849) kits (Lifecodes, Stanford, CT). The seven loci met the Hardy–Weinberg expectations, and they possess a combined power discrimination higher than 0.999999999 and a combined power exclusion similar to 0.9996, showing them to be a group of excellent markers as complement in paternity testing in the identification of individuals and in forensic applications in general.

Polymarker, HLA-DQAα, and D1S80 Allele Frequency Data in Antioquia (Colombia) Population

Blood samples from unrelated Caucasian individuals from Colombia (Antioquia) were collected. DNA was extracted from 200 uL of peripherical blood obtained from each individual by the salting-out procedure (1).