Marcelo Ismar Silva Santana

Distribuição configurada pela artéria celíaca em papagaios-verdadeiros (Amazona aestiva)

This research was performed aiming to describe the origin and the distribution of the celiac artery in 20 blue fronted amazon (Amazona aestiva), through the completion of their circulatory system with an aqueous solution of colored latex, followed by fixation in formol for later dissection of the collateral branches. The first branch emitted the proventricular dorsal artery that headed to the ventricle as gastric dorsal artery and next emitted the proventricular ventral artery which originated on the celiac artery in 85% of the cases and in 15% had its origin on the left branch of the celiac artery. In 25% of the cases the splenic artery was emitted before the bifurcation on left and right branches. The left branch was responsible for the origin of the proventricular branches, piloric branches, splenic artery and proventricular artery, which emitted branches to the proventricle and esophagus. The celiac artery also emitted the gastric ventral artery and left gastric. The right branch emitted splenic arteries (that varied from a number of seven); duodenal bound for the more caudal segment of the ascendant segment of the duodenum and the right hepatica to the right liver lobe. On its terminal segment the right branch was divided into right gastric arteries to the ventricle and pancreaticduodenal to the pancreas, ascendant and descendant braces of the duodenum and to the cranial segments of the ileum and caudal of the jejune. The irrigation provided by the celiac artery in parrots remains the same as described for other bird genders showing as divergences: the lack of vesicular arteries and ileocecal as well the gall bladder and the cecos.

Morphological description of male genital organs of Marca's marmoset (Mico marcai)

Morphological characterisation of the genital organs of primates may bring significant contributions to the understanding of different reproductive behaviours and support new conservation strategies. However, relevant or detailed descriptions of genital morphology of several primate species are still lacking. This study describes the gross and microscopic anatomy of the internal and external genitalia of Marcas marmoset (Mico marcai). The same organs described in other primate species were identified here, but some anatomical particularities were detected, such as absence of a dartos tunic, presence of a vas deferens ampulla, absence of spongious erectile tissue in the pelvic urethra, separation of prostate gland lobes by a longitudinal sulcus and lack of septation in the corpus cavernosus and spongiosus at the level of the shaft and free portion of the penis. Keratinised type 1 spicules arising from epidermal or dermal projections were found in the free portion of the penis. Microscopic analysis revealed a small bone (baculum) consisting of peripheral compact bone and a central, non‐ossified area filled with vascular tissue at the distal end of this portion of the penis. Results of this study may support further comparative studies of primates reproductive ecology.

Descrição anatômica de esqueletos de papagaios do gênero Amazona através da utilização de radiografias

This study describes the skeleton of Amazona aestiva parrots and compared it with representatives of other Amazona species. To this end, we used 22 specimens of Amazona aestiva, two specimens each of Amazona vinacea; Amazona rhodocorythae, Amazona farinosa and one specimen each of Amazona brasiliensis and Amazona pretrei donated after natural death by Pocos de Caldas Breeding Park. Full body radiographs were taken, in either the right or left lateral decubitus position, in the case of laterolateral projections, and in supine position, in the case of ventrodorsal projection. Regardless of the species, skulls of the parrots were classified as prokinetic, because of free movements in their rostral portion. In most cases, the spine consisted of: 12 cervical vertebrae, six free thoracic vertebrae, synsacrum (formed by the fusion of the last thoracic vertebrae, 7 lumbosacral vertebrae and one caudal vertebrae), five free caudal vertebrae and the pygostyle (formed by three fused caudal vertebrae). Despite punctual differences, thoracic and pelvic appendicular skeleton proved to be very similar to that observed for other genera of birds. In addition, there was no sexual dimorphism considering the anatomical characteristics of the skeletons of parrots analyzed.

Density of primary and secondary epidermal laminae of equine hoof

Differences in the microscopic morphology of the hoof in forelimbs and hindlimbs of horses have been scarcely reported in the literature, especially concerning the distribution of primary and secondary epidermal laminae in the different regions. This study aimed to determine the density of primary and secondary epidermal laminae in the hoof of horses. For this, it was used fore and hindlimbs of 16 adult mixed breed horses. With a cross section 0.5 cm above the sole, it was quantified the primary epidermal laminae in the regions of the toe, and of lateral and medial quarters. Fragments with about 1cm ³ were taken from the proximal, middle and distal thirds of the hooves, in the different regions, subjected to conventional histological techniques and examined with an optical microscope. Data were statistically analyzed in relation to the fore and hindlimbs and between their various regions. The density of primary epidermal laminae varied around the hoof circumference, with greater values in the hoof toe, which gradually decreased towards the bulb of the hoof, without difference between thoracic and pelvic limbs. The average density of the secondary epidermal laminae per primary epidermal lamina does not change around the circumference of the hoof. Our findings indicated that the density of epidermal laminae is not different between fore and hindlimbs. The variation in the density of primary epidermal laminae around the hoof seems to be part of an adaptive response to different stresses in each region. A better understanding of the structural morphology contributes to a better understanding of the diagnosis, pathophysiology, and treatment of disorders that affect the hoof.

Quantificação de tecido conjuntivo do músculo cardíaco em equinos de tração através de técnicas histoquímicas e morfométricas

ABSTRACT Cardiomyocytes are sustained and inserted into aconnective tissue skeleton that has different distributiondepending up on the properties of the region in which it stands.The purpose of this study was to quantify the proportion ofconnective tissue in relation to the arrangement ofcardiomyocytes of left and right ventricles and interventricularseptum in myocardium of six mixed breed, ill trifted adulthorses, of both sexes, used for traction. With hearts still freshand with the aid of a digital caliper, it was estimated the heightof left ventricle, the heart’s width, the heart’s circumferenceand the thickness of the ventricular free walls and interventricularseptum. The fragments on the middle third of the interventricularseptum and free walls of the ventricles were subjected toconventional histological technique. The blocks were cut withthickness of 5 μ m and stained with Picrosirius Red, GomoriTrichrome and Azan Trichrome to show the connectivetissue. The slides were analyzed using optical microscopecoupled to a digital analysis program Image-Pro Plus®. Theaverage proportion of connective tissue in the left ventricle was6.1+3.7%, in the interventricular septum and it was observed amean of 6.8+3.6%. In the right ventricle the average was6.0+3.0%. Applying the H test of Kruskal-Wallis, it was foundthat there was statistical difference between the different dyesused for each region. In the Pearson correlation test it was notfound pattern correlation between the thickness of the regionsanalyzed and the proportion of connective tissue