Marcos Célio de Almeida

A simple method for human peripheral blood monocyte Isolation

We describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy coat cells. The use of simple laboratory equipment and a relatively cheap reagent makes the described method a convenient approach to obtaining human monocytes.

Caso fatal de adiaspiromicose pulmonar humana

A fatal case of human pulmonary adiaspiromycosis is reported. The patient, a male farm laborer from Pernambuco, in the Northeastern part of Brazil, had moved to Planaltina, DF., one year prior the onset of his illness. The main clinical manifestations consisted in fever, myalgia, cough and dyspnea. After being sick for five weeks, the patient developed severe respiratory failure and died. Grossly, disseminated nodular lesions, a few millimeters in diameter, were observed throughout the lobes of both lungs. Microscopically, round structures, up to 600 /mi in diameter, with a thick membrane, identified as adiaconidia of Chrysosporium parvum var. crescens, were seen in the center of the nodular lesions. These adiaconidia appeared predominantly inside microabscesses or necrotic areas, both surrounded by a granulomatous reaction. The pulmonary alveoli not affected by the nodular lesions contained an inflammatory exudate composed of macrophages and neutrophils. The finding of several non-fatal cases of the disease in the area of the Federal District suggests that adiaspiromycosis is endemic in the Central Brazilian Plateau region, where the dry, warm and windy climate, particulary from August to October, may provide the appropriate environmental conditions for the dissemination of C. parvum conidia.

Correlação diagnóstica anatomoclínica. Aferição retrospectiva do diagnóstico clínico em necrópsias

Analisaram-se retrospectivamente 200 necropsias, correlacionando-se os diagnosticos clinicos e anatomopatologicos. Foram consideradas 20 entidades nosologicas para confronto. Os diagnosticos nao formulados em vida, embora observados na necropsia (falso negativo), e que apresentaram os maiores percentuais em relacao aos respectivos totais de achados necroscopicos, foram: pielonefrite aguda (100%), embolia pulmonar (87,50%), aneurisma dissecante da aorta (66,66%) e broncopneumonia (58,16%). Os diagnosticos formulados em vida que nao tiveram confirmacao pela necropsia (falso positivo), e que apresentaram os maiores percentuais em relacao aos respectivos totais dos diagnosticos clinicos, foram: tuberculose (69,50%), paracoccidioidomicose (57,14%), septicemia (53,13%) e doenca de Chagas (44,44%). Houve concordância diagnostica em 97 (48,50%) dos 200 casos. Em 19 (9,50%) deles o desacordo diagnostico, se antes verificado, poderia ter implicado alteracoes do prognostico. Enfatiza-se a importância da necropsia para uma adequada correlacao anatomoclinica e discute-se os achados em relacao a estudos previos.

Dextran-functionalized magnetic fluid mediating magnetohyperthermia for treatment of Ehrlich-solid-tumor-bearing mice: toxicological and histopathological evaluations

Dextran-functionalized maghemite fluid (DexMF) has been tested to treat Ehrlich-solid-tumor-bearing mice, evidencing its potential use in mediating magnetohyperthermia in breast cancer treatment. However, although magnetic nanoparticles tend to accumulate in tumor tissues, part of the nanomaterial can reach the blood stream, and then the organism. The aim of this study was to investigate the acute systemic effects of the intratumoral injection of DexMF mediating magnetohyperthermia in the treatment of an advanced clinical Ehrlich-solid-tumor, assessed through histopathological analyses of liver, kidneys, heart and spleen, comet assay, micronucleus test, hemogram, and serum levels of bilirubin, aspartate aminotransferase, alanine aminotransferase, gamma glutamyl transferase, alkaline phosphatase, creatinine, and urea. The tumor’s histopathology and morphometry were used to assess its aggressiveness and regression. DexMF mediating hyperthermia was effective in containing tumor aggressiveness and in inducing tumor regression, besides showing no toxic effects. Its physical characteristics also suggest that it is safe to use in other biomedical applications.

Crista supraventricularis Purkinje network and its relation to intraseptal Purkinje Network

Using transparent specimens with a dual color injection, microscopy, and computer tomography, this report shows that the right and left ventricular subendocardial Purkinje networks are connected by an extensive septal network in the bovine heart. The septal network is present along the entire septum except at a free zone below ventricular valves. Being the only communication of the basal right septum with the right free wall, the supraventricular crest is an enigmatic but not, by any means, hidden muscular structure. It is one of the last structures to be activated in human heart. It is shown here that the supraventricular crest Purkinje network connects the anterosuperior right ventricular basal free wall Purkinje network to anterior right ventricular basal septal Purkinje network. It is suggested that the stimulus initiated at middle left ventricular endocardium will activate the supraventricular crest. The intraseptal connection found between the basal left ventricular subendocardial septal Purkinje network and the right ventricular basal septal Purkinje network is, probably, the pathway for the stimulus. An anatomic basis is provided to explain why the inflow tract contracts earlier than the outflow tract in the right ventricle systole. Anat Rec, 2017.

Histoplasma com derrame pleural: relato de um caso

Pleural effusions are rare events accompanying histoplasmosis, and in general they are associated with acute forms of the disease. It is described here a case of histoplasmoma associated with pleuritic pain and pleural effusion.Efusoes pleurais surgem raramente em associacao com a histoplasmose capsu-lata, ocorrendo em geral nas formas agudas da doenca. Relatamos e discutimos um caso clinico em que um histoplasmoma subpleural acompanhou-se de dor pleuritica, hidrotorax e pleurite fibrosante.Efusoes pleurais surgem raramente em associacao com a histoplasmose capsu-lata, ocorrendo em geral nas formas agudas da doenca. Relatamos e discutimos um caso clinico em que um histoplasmoma subpleural acompanhou-se de dor pleuritica, hidrotorax e pleurite fibrosante.

Histoplasmoma and pleural effusion: a case report

Pleural effusions are rare events accompanying histoplasmosis, and in general they are associated with acute forms of the disease. It is described here a case of histoplasmoma associated with pleuritic pain and pleural effusion.Efusoes pleurais surgem raramente em associacao com a histoplasmose capsu-lata, ocorrendo em geral nas formas agudas da doenca. Relatamos e discutimos um caso clinico em que um histoplasmoma subpleural acompanhou-se de dor pleuritica, hidrotorax e pleurite fibrosante.Efusoes pleurais surgem raramente em associacao com a histoplasmose capsu-lata, ocorrendo em geral nas formas agudas da doenca. Relatamos e discutimos um caso clinico em que um histoplasmoma subpleural acompanhou-se de dor pleuritica, hidrotorax e pleurite fibrosante.