Marcos Mandaji

Performance of an ultraviolet light‐emitting diode‐induced fluorescence detector in capillary electrophoresis

The performance of a fluorescence detector in capillary electrophoresis (CE) using a light‐emitting diode (LED) as excitation source is reported. An ultraviolet LED pulsed at a repetition rate of 500 Hz, combined with a time‐discrimination and averaging acquisition system, was used. Limits of detection of 3 and 18 fmoles (at a signal‐to‐noise ratio equal to 3) were achieved for fluorescamine‐derivatized bradykinin and lysine, respectively. This system exhibited a linear response for a concentration range between 54 and 417 νM for derivatized lysine, and between 1.81 and 23.58 νM for derivatized bradykinin. This detection system showed to be very convenient for routine analytical applications.

Sample stacking in CZE using dynamic thermal junctions I. Analytes with low dpKa/dT crossing a single thermally induced pH junction in a BGE with high dpH/dT

The possibility to compress analyte bands at the beginning of CE runs has many advantages. Analytes at low concentration can be analyzed with high signal‐to‐noise ratios by using the so‐called sample stacking methods. Moreover, sample injections with very narrow initial band widths (small initial standard deviations) are sometimes useful, especially if high resolutions among the bands are required in the shortest run time. In the present work, a method of sample stacking is proposed and demonstrated. It is based on BGEs with high thermal sensitive pHs (high dpH/dT) and analytes with low dpKa/dT. High thermal sensitivity means that the working pKa of the BGE has a high dpKa/dT in modulus. For instance, Tris and Ethanolamine have dpH/dT=−0.028/°C and −0.029/°C, respectively, whereas carboxylic acids have low dpKa/dT values, i.e. in the −0.002/°C to+0.002/°C range. The action of cooling and heating sections along the capillary during the runs affects also the local viscosity, conductivity, and electric field strength. The effect of these variables on electrophoretic velocity and band compression is theoretically calculated using a simple model. Finally, this stacking method was demonstrated for amino acids derivatized with naphthalene‐2,3‐dicarboxaldehyde and fluorescamine using a temperature difference of 70°C between two neighbor sections and Tris as separation buffer. In this case, the BGE has a high pH thermal coefficient whereas the carboxylic groups of the analytes have low pKa thermal coefficients. The application of these dynamic thermal gradients increased peak height by a factor of two (and decreased the standard deviations of peaks by a factor of two) of aspartic acid and glutamic acid derivatized with naphthalene‐2,3‐dicarboxaldehyde and serine derivatized with fluorescamine. The effect of thermal compression of bands was not observed when runs were accomplished using phosphate buffer at pH 7 (negative control). Phosphate has a low dpH/dT in this pH range, similar to the dKa/dT of analytes. It is shown that ∣dKa/dT−dpH/dT∣≫0 is one determinant factor to have significant stacking produced by dynamic thermal junctions.

Sample stacking in CZE using dynamic thermal junctions II: Analytes with high dpKa/dT crossing a single thermal junction in a BGE with low dpH/dT

In a previous work [M. Mandaji, et al., this issue] a sample stacking method was theoretically modeled and experimentally demonstrated for analytes with low dpKa/dT (analytes carrying carboxylic groups) and BGEs with high dpH/dT (high pH–temperature‐coefficients). In that work, buffer pH was modulated with temperature, inducing electrophoretic mobility changes in the analytes. In the present work, the opposite conditions are studied and tested, i.e. analytes with high dpKa/dT and BGEs that exhibit low dpH/dT. It is well known that organic bases such as amines, imidazoles, and benzimidazoles exhibit high dpKa/dT. Temperature variations induce instantaneous changes on the basicity of these and other basic groups. Therefore, the electrophoretic velocity of some analytes changes abruptly when temperature variations are applied along the capillary. This is true only if BGE pH remains constant or if it changes in the opposite direction of pKa of the analyte. The presence of hot and cold sections along the capillary also affects local viscosity, conductivity, and electric field strength. The effect of these variables on electrophoretic velocity and band stacking efficacy was also taken into account in the theoretical model presented. Finally, this stacking method is demonstrated for lysine partially derivatized with naphthalene‐2,3‐dicarboxaldehyde. In this case, the amino group of the lateral chain was left underivatized and only the alpha amino group was derivatized. Therefore, the basicity of the lateral amino group, and consequently the electrophoretic mobility, was modulated with temperature while the pH of the buffer used remained unchanged.