Margit Szabó

Auxin autotrophic tobacco callus tissues resist oxidative stress: The importance of glutathione S-transferase and glutathione peroxidase activities in auxin heterotrophic and autotrophic calli

Auxin autotrophic and heterotrophic tobacco callus lines were grown on MS medium with or without 100 mmol/L NaCl and growth and some of the stress-related activities, such as GPX, SOD, CAT, GST, GSH-PX, as well as the concentration of ethylene and H2O2, were measured and compared with each other. The auxin autotrophic calli grew slower, however, on the NaCl-containing medium the growth rate was higher than that of the heterotrophic cultures after two weeks of culturing. The stress-related ethylene production was lower in the autotrophic cultures and, contrary to the heterotrophic tissues, its level did not change significantly upon NaCl treatment. The guaiacol peroxidase (GPX) activities were higher in the autotrophic tissues in all cell fractions regardless of the presence of NaCl. Treated with NaCl, the GPX activities elevated in the soluble and covalently-bound fractions in the heterotrophic calli, but were not further increased in the autotrophic line. SOD and CAT activities were higher in the heterotrophic tissues, and were increased further by 100 mmol/L NaCl treatment. The GST and GSH-PX activities were higher in the autotrophic line, which might explain their enhanced stress tolerance. In the autotrophic tissues, the elevated antioxidant activities led to reduced levels of H2O2 and malondialdehyde; under mild NaCl stress, these levels decreased further. The lower growth rate and the effective protection against NaCl stress-induced oxidative damage of the autotrophic line can be explained by the cell wall-bound peroxidase and GSH-PX activities in the auxin autotrophic tissues. Their maintained growth rate indicates that the autotropic cultures were more resistant to exogenous H2O2.