María del Rosario Robles

Helminth communities and host- parasite relationships in argentine brown rat (Rattus norvegicus)

SummaryThe aims of this research were: 1) to determine the helminth parasite fauna of seventy two Rattus norvegicus (Berkenhout, 1769) individuals inhabiting poultry farms in the Pampean region of Argentina; 2) to examine the relationship between parasitism and individual characteristics of the hosts, and 3) to analyze the associations among helminths. The study was carried out in twenty-four poultry farms from autumn 2000 to winter 2001. Nematodes were found in the intestine or caecum rectum of the 74 % of rats and cestodes were found along all the intestine of the 28 % of rats. Heterakis spumosa (Travassos 1914) was a central species, whereas Syphacia muris, Yamaguti 1941, Nippostrongylus brasiliensis (Travassos 1914) and Hymenolepis diminuta (Rudolphi 1819) were satellite species. Helminths do not affect rat survival and growth of rats. Rats parasitized with H. spumosa and H. diminuta were larger and older than non-parasitized rats. The intensity of infection with H. spumosa significantly increased with rat age. Rats parasitized with Nippostrongylus brasiliensis and Syphacia muris did not differ in size and age with respect to non-parasitized ones. The mean intensity of infections per host was 33.74, 74.28, higher than 200, and 3.10 for H. spumosa, S. muris, N. brasiliensis and H. diminuta, respectively. The mean intensity of infection with H. spumosa was higher in summer than in the other seasons, while the higher mean intensity of infection with N. brasiliensis and S. muris occurred in autumn. Infections with Heterakis spumosa and Syphacia muris, and with Syphacia muris and Nippostrongylus brasiliensis were independent; while Heterakis spumosa and Nippostrongylus brasiliensis were negatively associated.

A NEW SPECIES OF TRICHURIS (NEMATODA: TRICHURIDAE) FROM PHYLLOTINI RODENTS IN ARGENTINA

A new species of Trichuris is described. Trichuris pardinasi n. sp. was recovered from Phyllotis xanthopygus Waterhouse (Rodentia: Muridae: Phyllotini) in Sierra de la Ventana, Buenos Aires Province, and Pampa de Achala, Córdoba Province (Argentina). This is the first record of Trichuris parasitizing Phyllotini rodents. The new species can be differentiated from the other 10 species parasitizing rodents from South America by the absence of the spicular tube, spicular sheath with spines uniformly distributed, the length of spicule, the J-shaped proximal cloacal tube, and the nonprotrusive vulva. Also, a description of the bacillary band is provided. The present and the future findings of shared parasite fauna from both populations of P. xanthopygus in these disjunct areas will support the hypothesis of a continuous distribution of this host species at a past time.

New Species of Trichuris (Nematoda: Trichuridae) from Akodon montensis Thomas, 1913, of the Paranaense Forest in Argentina

Abstract Species of Trichuris (Nematoda: Trichuridae) parasitize a broad range of mammalian hosts. To date, 21 Trichuris species infecting nine families of rodents have been found in North and South America. Trichuris navonae n. sp. is described on the basis of specimens recovered from a species of forest-dwelling mice, Akodon montensis (Cricetidae: Sigmodontinae), from nine localities of Misiones Province, Argentina. A comparison with all the species of Trichuris from North and South American rodents is given. The separation of the new species of Trichuris is based on morphologic and morphometrica features, such as the absence of a spicular tube, the presence of a cylindrical spicular sheath with sharp spines, a non-protrusive vulva, a long anterior-posterior portion of the body, a lengthy spicule, and a proximal and distal cloacal tube. This is the third record of this genus in rodents of the Sigmodontinae from Argentina and the fifth record from South American rodents. Despite the large number of potential host species, only about 1.9% of sigmodontine rodent species have been reported as hosts of Trichuris spp. It is suggested that this number represents but a small fraction of Trichuris spp. that occur in sigmodontine rodents, and that additional survey of this group should yield additional species.

A new species of Syphacia (Nematoda: Oxyuridae) from Akodon azarae (Rodentia: Cricetidae) in Argentina.

Eight species of Syphacia (Nematoda: Oxyuridae) have been reported from South America in rodents of the Sigmodontinae, only 1 of which has been recorded in Argentina. Syphacia (Syphacia) carlitosi n. sp. is described from the ceca of Akodon azarae bibianae and Akodon azarae hunteri (Sigmodontinae: Akodontini) captured in 3 provinces in the northeast region of Argentina. The new species is differentiated principally by the shape of the cephalic plate; distribution of submedian papillae and amphids; presence, extent, and shape of cervical alae in females; absence of lateral alae; absence of deirids; spicular and gubernaculum length; shape and structure of accessory hook of gubernaculum; and distance of mamelons, excretory pore, and vulva from the anterior extremity. This is the second record of Syphacia parasitizing rodents of the tribe Akodontini.

Redescription of Trichuris laevitestis (Nematoda: Trichuridae) from Akodon azarae and Scapteromys aquaticus (Sigmodontinae: Cricetidae) in Buenos Aires province, Argentina

Trichuris spp. have a cosmopolitan distribution; the species are typically identified by features related to the reproductive system. Trichuris laevitestis was described on the basis of specimens collected from rodents captured in Punta Lara, Buenos Aires Province, Argentina. Here, T. laevitestis is redescribed considering new and relevant morphological features of diagnostic importance such as arrangement of spines on the spicular sheath, spicule length, length and shape of proximal cloacal tube, paracloacal papillae, features of the vulva, and ratios between different features. Akodon azarae and Scapteromys aquaticus specimens captured in the Rio de la Plata and Paraná Guazú River, and at Cerro de la Gloria, Buenos Aires Province, Argentina, were examined. The bacillary band of T. laevitestis is described, and several morphological details of this species are illustrated and recorded by scanning electron microscopy for the first time. The redescription of T. laevitestis confirms its identity as a valid species. In addition, the present study extends the geographical distribution of the species.

A New Angiostrongylid (Nematoda) Species from the Pulmonary Arteries of Akodon azarae (Rodentia: Cricetidae) in Argentina

Angiostrongylus morerai n. sp. (Nematoda: Angiostrongylidae) is described from the pulmonary arteries of Azaras grass mouse Akodon azarae (Rodentia: Cricetidae) in Argentina. It is distinguished from its congeners principally by the morphology of the dorsal ray, which is as long, or longer, than the externodorsals and has 2 long branches; the spicule lengths are also greater (400–465 μm). This is the first record of a metastrongyloid from sigmodontine rodents in Argentina.

New Morphological Details and First Records of Heterakis spumosa and Syphacia muris from Argentina

Abstract Heterakis spumosa and Syphacia muris are among the most common species parasitizing mammals. Although these nematodes have been reported from many hosts on several continents, in South America, they have only been recovered from the cecum of Rattus norvegicus from Brazil and Peru. This study provides morphological details of H. spumosa and S. muris. Moreover, this is the first record of these species in Argentina. Heterakis spumosa and S. muris were collected from specimens of R. norvegicus from Partido de Exaltación de la Cruz, Buenos Aires Province, Argentina. This note presents relevant features observed with scanning electron microscopy (SEM) for H. spumosa, such as the presence of 4 papillae on the lips, the lateral alae forming a cuticular fold inside a groove that extends to the posterior end, double papillae on the tip of tail of the male, vulva with 5 cuticular processes, and 2 pair of sessile papillae on the female tail. Syphacia muris has a porous badge posterior to the amphids, and the excretory pore is located in an oval depression.

A new species of Syphacia (Nematoda: Oxyuridae) from Oligoryzomys nigripes (Rodentia: Cricetidae) in Argentina

The aim of this paper is to describe Syphacia kinsellai n. sp. (Nematoda: Oxyuridae) from the cecum of Oligoryzomys nigripes (Olfers 1818) (Sigmodontinae: Oryzomyini) captured in Misiones Province, Argentina. The new species can be differentiated from other species of the genus principally by the shape of the cephalic plate; distribution of submedian papillae and amphids; development of porous badge; presence of derids in females; absence of cervical and lateral alae; shape and structure of accessory hook of gubernaculum; and distance of excretory pore and vulva from the anterior extremity. Until the present, only two species of Syphacia had been reported from Argentina in sigmodontine rodents, the first one parasitizes an Oryzomyini host and the second one an Akodontini host. This new species is the second record of Syphacia from the tribe Oryzomyini in Argentina; however, we propose that the first record, S. oryzomae, should be treated as a nomen dubium.

Redescription of Syphacia venteli Travassos 1937 (Nematoda: Oxyuridae) from Nectomys squamipes in Argentina and Brazil and description of a new species of Syphacia from Melanomys caliginosus in Colombia

Syphacia venteli Travassos, Mem Inst Oswaldo Cruz 32:607–613, 1937 is redescribed on the basis of specimens recovered from the type host, Nectomys squamipes (Brants 1827), from Brazil and Argentina. Specimens determined by Quentin (Bull Mus Natl Hist Nat 2:909–925, 1969) as S. venteli from Melanomys caliginosus (Tomes 1860) in Colombia were re-studied and assigned to a new species. In both species, structures such as the shape of the cephalic plate, details and distribution of the submedian papillae and amphids, presence and absence of the lateral and cervical alae, and shape and structure of the accessory hook of the gubernaculum were studied with the light microscope and scanning electron microscope. The present survey is the first detailed study of the species S. venteli since the original description, and the first record of this species from Argentina. Moreover, the present study suggests that the validity of some host species of Syphacia should be questioned and that through of the study of deposited specimens, a more exact number of parasitized host species can be confirmed, contributing to a better understanding of host specificity in this genus.

New host, geographic records, and histopathologic studies of Angiostrongylus spp (Nematoda: Angiostrongylidae) in rodents from Argentina with updated summary of records from rodent hosts and host specificity assessment

To date, 21 species of the genus Angiostrongylus (Nematoda: Angiostrongylidae) have been reported around the world, 15 of which are parasites of rodents. In this study, new host, geographic records, and histopathologic studies of Angiostrongylus spp in sigmodontine rodents from Argentina, with an updated summary of records from rodent hosts and host specificity assessment, are provided. Records of Angiostrongylus costaricensis from Akodon montensis andAngiostrongylus morerai from six new hosts and geographical localities in Argentina are reported. The gross and histopathologic changes in the lungs of the host species due to angiostrongylosis are described. Published records of the genus Angiostrongylus from rodents and patterns of host specificity are presented. Individual Angiostrongylusspecies parasitise between one-19 different host species. The most frequent values of the specificity index (STD) were between 1-5.97. The elevated number of host species (n = 7) of A. morerai with a STD = 1.86 is a reflection of multiple systematic studies of parasites from sigmodontine rodents in the area of Cuenca del Plata, Argentina, showing that an increase in sampling effort can result in new findings. The combination of low host specificity and a wide geographic distribution of Angiostrongylus spp indicates a troubling epidemiological scenario although, as yet, no human cases have been reported.