Maria Olga Kokornaczyk

Self-organized crystallization patterns from evaporating droplets of common wheat grain leakages as a potential tool for quality analysis.

We studied the evaporation-induced pattern formation in droplets of common wheat kernel leakages prepared out of ancient and modern wheat cultivars as a possible tool for wheat quality analysis. The experiments showed that the substances which passed into the water during the soaking of the kernels created crystalline structures with different degrees of complexity while the droplets were evaporating. The forms ranged from spots and simple structures with single ramifications, through dendrites, up to highly organized hexagonal shapes and fractal-like structures. The patterns were observed and photographed using dark field microscopy in small magnifications. The evaluation of the patterns was performed both visually and by means of the fractal dimension analysis. From the results, it can be inferred that the wheat cultivars differed in their pattern-forming capacities. Two of the analyzed wheat cultivars showed poor pattern formation, whereas another two created well-formed and complex patterns. Additionally, the wheat cultivars were analyzed for their vigor by means of the germination test and measurement of the electrical conductivity of the grain leakages. The results showed that the more vigorous cultivars also created more complex patterns, whereas the weaker cultivars created predominantly poor forms. This observation suggests a correlation between the wheat seed quality and droplet evaporation patterns.

Droplet evaporation method as a new potential approach for highlighting the effectiveness of ultra high dilutions

OBJECTIVE This study sought to verify whether the droplet evaporation method (DEM) can be applied to assess the effectiveness of ultra-high dilutions (UHDs). We studied the shape characteristics of the polycrystalline structures formed during droplet evaporation of wheat seed leakages. METHODS The experimental protocol tested both unstressed seeds and seeds stressed with arsenic trioxide 5mM, treated with either ultra-high dilutions of the same stressor substance, or with water as a control. The experimental groups were analyzed by DEM and in vitro growth tests. DEM patterns were evaluated for their local connected fractal dimension (measure of complexity) and fluctuating asymmetry (measure of symmetry exactness). RESULTS Treatment with arsenic at UHD of both stressed and non-stressed seeds increased the local connected fractal dimension levels and bilateral symmetry exactness values in the polycrystalline structures, as compared to the water treatment. The results of in vitro growth tests revealed a stimulating effect of arsenic at UHD vs. control, and a correlation between the changes in growth rate and the crystallographic values of the polycrystalline structures was observed. CONCLUSIONS The results indicate that polycrystalline structures are sensitive to UHDs, and so for the first time provide grounds for the use of DEM as a new tool for testing UHD effectiveness. DEM could find application as a treatment pre-selection tool, or to monitor sample conditions during treatment. Moreover, when applied to biological liquids (such as saliva, blood, blood serum, etc.), DEM might provide information about UHD effectiveness on human and animal health.

Status quo and future research challenges on organic food quality determination with focus on laboratory methods

Organic food quality determination needs multi-dimensional evaluation tools. The main focus is on the authentication as an analytical verification of the certification process. New fingerprinting approaches such as ultra-performance liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry, direct analysis in real time-high-resolution mass spectrometry as well as crystallization with and without the presence of additives seem to be promising methods in terms of time of analysis and detecting organic system-related parameters. For further methodological development, a system approach is recommended, which also takes into account food structure aspects. Furthermore, the authentication of processed organic samples needs more consciousness, hence most of organic food is complex and processed.

Qualitative discrimination between organic and biodynamic Sangiovese red wines for authenticity

In this study the ability of the droplet evaporation method (DEM) to discriminate between organic and biodynamic Sangiovese red wines was studied for the first time. The relationship between the sizing-parameters and shape-descriptors of the crystal structures and the chemical parameters of wines was evaluated, and the most significant correlations were found between the intensity of red color of wines with circularity (r = 0.90) and solidity (r = 0.94). Moreover the shape-descriptors allowed a discrimination between the organic and biodynamic Sangiovese red wines based on two kinds of structures: (i) needle-like and (ii) flower-like forms.

Phase-Transition-Induced Pattern Formation Applied to Basic Research on Homeopathy: A Systematic Review

BACKGROUND Methods based on phase-transition-induced pattern formation (PTPF) are increasingly used in medical research. Frequent application fields are medical diagnosis and basic research in homeopathy. Here, we present a systematic review of experimental studies concerning PTPF-based methods applied to homeopathy research. We also aimed at categorizing the PTPF methods included in this review. MATERIALS AND METHODS Experimental studies were collected from scientific databases (PubMed, Web of Science, Russian eLibrary) and from experts in the research field in question, following the PRISMA guidelines. The studies were rated according to pre-defined scientific criteria. RESULTS The review included 15 experimental studies. We identified seven different PTPF methods applied in 12 experimental models. Among these methods, phase-transition was triggered through evaporation, freezing, or solution, and in most cases led to the formation of crystals. First experimental studies concerning the application of PTPF methods in homeopathic research were performed in the first half of the 20th century; however, they were not continued in the following years. Only in the last decade, different research groups re-launched the idea, introducing new experimental approaches and computerized pattern evaluation techniques. The here-identified PTPF methods are for the first time proposed to be classified as one group of methods based on the same basic physical phenomenon. CONCLUSIONS Although the number of experimental studies in the area is still rather limited, the long tradition in the application of PTPF methods and the dynamics of the present developments point out the high potential of these methods and indicate that they might meet the demand for scientific methods to study potentized preparations.

Analysis of soils by means of Pfeiffer’s circular chromatography test and comparison to chemical analysis results

Abstract Pfeiffer’s circular chromatography (PCC) is an analytical method relying on paper chromatography principles and applied mainly to test the quality of soils. The output of PCC are colored patterns formed on circular filter papers, pretreated with a photosensitive substance, and imbibed with a NaOH aqueous extract of the soil sample. In this study ( performed in frames of the project ‘Panis cum Carne’ financed by the Umbria Region, Italy) 16 soil samples from differently managed fields and planted with different crops were analysed by means of PCC and standard chemical analysis. The PCC pattern evaluation consisted of three approaches: (i) measurement of pattern zones, (ii) visual scoring of pattern characteristics by schooled evaluators, and (iii) computerised texture analysis. Subsequently the collected PCC data were correlated with the chemical soil characteristics; there were strong significant correlations with the contents of organic matter, total nitrogen, and assimilable phosphorus and bromine. The results of this study provide evidence that PCC patterns may provide an overall and reliable view on the soil state.