Maria Stenberg

Soil Resources Influence Spatial Patterns of Denitrifying Communities at Scales Compatible with Land Management

ABSTRACT Knowing spatial patterns of functional microbial guilds can increase our understanding of the relationships between microbial community ecology and ecosystem functions. Using geostatistical modeling to map spatial patterns, we explored the distribution of the community structure, size, and activity of one functional group in N cycling, the denitrifiers, in relation to 23 soil parameters over a 44-ha farm divided into one organic and one integrated crop production system. The denitrifiers were targeted by the nirS and nirK genes that encode the two mutually exclusive types of nitrite reductases, the cd1 heme-type and copper reductases, respectively. The spatial pattern of the denitrification activity genes was reflected by the maps of the abundances of nir genes. For the community structure, only the maps of the nirS community were related to the activity. The activity was correlated with nitrate and dissolved organic nitrogen and carbon, whereas the gene pools for denitrification, in terms of size and composition, were influenced by the soil structure. For the nirS community, pH and soil nutrients were also important in shaping the community. The only unique parameter related to the nirK community was the soil Cu content. However, the spatial pattern of the nirK denitrifiers corresponded to the division of the farm into the two cropping systems. The different community patterns, together with the spatial distribution of the nirS/nirK abundance ratio, suggest habitat selection on the nirS- and nirK-type denitrifiers. Our findings constitute a first step in identifying niches for denitrifiers at scales relevant to land management.

Spatial distribution of ammonia-oxidizing bacteria and archaea across a 44-hectare farm related to ecosystem functioning.

Characterization of spatial patterns of functional microbial communities could facilitate the understanding of the relationships between the ecology of microbial communities, the biogeochemical processes they perform and the corresponding ecosystem functions. Because of the important role the ammonia-oxidizing bacteria (AOB) and archaea (AOA) have in nitrogen cycling and nitrate leaching, we explored the spatial distribution of their activity, abundance and community composition across a 44-ha large farm divided into an organic and an integrated farming system. The spatial patterns were mapped by geostatistical modeling and correlations to soil properties and ecosystem functioning in terms of nitrate leaching were determined. All measured community components for both AOB and AOA exhibited spatial patterns at the hectare scale. The patchy patterns of community structures did not reflect the farming systems, but the AOB community was weakly related to differences in soil pH and moisture, whereas the AOA community to differences in soil pH and clay content. Soil properties related differently to the size of the communities, with soil organic carbon and total nitrogen correlating positively to AOB abundance, while clay content and pH showed a negative correlation to AOA abundance. Contrasting spatial patterns were observed for the abundance distributions of the two groups indicating that the AOB and AOA may occupy different niches in agro-ecosystems. In addition, the two communities correlated differently to community and ecosystem functions. Our results suggest that the AOA, not the AOB, were contributing to nitrate leaching at the site by providing substrate for the nitrite oxidizers.

Soil and crop responses to different tillage systems

An experiment was carried out over eight consecutive years at three sites, on clay or clay loam soils. In a split-plot design, two main treatments (mouldboard ploughing to 25 cm depth and disc or springtine cultivation to 13 cm depth) were combined with two seedbed preparation treatments (three passes with a conventional harrow vs. one pass with a power take off (PTO) driven harrow). Seedbed characteristics and bulk soil properties investigated at one of the sites in 1991 were similar in the different treatments in the 0-13 cm layer. In the 13-25 cm layer shallow cultivation resulted in significantly higher bulk density, degree of compactness and penetration resistance, and lower root density than in mouldboard ploughing. A reduced number of tractor passes achieved by using the PTO driven harrow resulted in significantly lower bulk density and penetration resistance in the unploughed soil, while still providing an adequate seedbed. At 25-30 cm depth, the volume of pores with equivalent diameter > 100 um, saturated hydraulic conductivity and air permeability were higher with ploughless tillage than with conventional tillage. Pore continuity was greater in unploughed soil at all depths investigated. In unploughed plots there was a concentration of organic carbon and potassium in the upper 13 cm. Phosphorus distribution and pH were not altered by the tillage systems. The yield was improved by the PTO driven harrow both in ploughed and unploughed plots.

Tile drain losses of nitrogen and phosphorus from fields under integrated and organic crop rotations. A four-year study on a clay soil in southwest Sweden.

In order to explore the influence of site-specific soil properties on nitrogen (N) and phosphorus (P) losses between individual fields and crop sequences, 16 drained fields with clay soils were investigated in a four-year study. Mean total N (TN) loss was 6.6-11.1 from a conventional, 14.3-21.5 from an organic and 13.1-23.9 kg ha(-1) year(-1) from an integrated cropping system across a 4 year period, with 75% in nitrate form (NO(3)-N). Mean total P (TP) loss was 0.96-3.03, 0.99-4.63 and 0.76-2.67 kg ha(-1) year(-1), from the three systems respectively during the same period, with 25% in dissolved reactive form (DRP). Median N efficiency was calculated to be 70% including gains from estimated N fixation. According to principal component factor (PCA) analysis, field characteristics and cropping system were generally more important for losses of N and P than year. Accumulation of soil mineral N in the autumn and (estimated) N fixation was important for N leaching. No P fertilisers were used at the site in either cropping system. Total P concentration in drainage water from each of the fields was marginally significantly (p<0.05) correlated to TP concentration in the topsoil (r=0.52), measured in hydrochloric acid extract (P-HCl). Mean DRP concentrations were significantly (p<0.01) correlated to degree of P saturation (DPS-AL) and soil carbon (C) content in the topsoil (r=0.63). Good establishment of a crop with efficient nutrient uptake and good soil structure was general preconditions for low nutrient leaching. Incorporation of ley by tillage operations in the summer before autumn crop establishment and repeated operations in autumn as well, increased N leaching. Crop management in sequences with leguminous crops needs to be considered carefully when designing cropping systems high efficiency in N utilisation and low environmental impact.

Reducing nitrate leaching after winter oilseed rape and peas in mild and cold winters

Nitrate leaching after winter oilseed rape and peas has not been studied at the most northern limits of oilseed rape cultivation where winters vary between being mild, with continuous drainage, and cold, with periods of frozen soil. Here, we studied the effect of N fertilisation to oilseed rape, catch crops after oilseed rape and peas and dired drilling of winter wheat after oilseed rape on N leaching in south-west Sweden. Nitrate leaching was determined in two field experiments, dated 2004–2006 and 2005–2007, respectively, on a sandy loam. Our results show that under oilseed rape nitrate leaching was low, at 16–23 kg N ha−1, in a mild winter with drainage from October to March. In the subsequent mild winter nitrate leaching under wheat was higher, amounting to 35–94 kg N ha−1. Nitrate leaching levels were similar, 32–58 kg N ha−1, for all crops in a cold winter with a long-lasting snow cover and main drainage occurring after snowmelt in March and April. Application of fertiliser N to oilseed rape at the optimum N rate, rather than 50 kg N ha−1 above optimum, reduced leaching in a following winter wheat crop by 25 and 27 kg N ha−1 in a cold and a mild winter, respectively. Spring undersowing of perennial ryegrass as a catch crop reduced leaching by 12 kg N ha−1 after optimally fertilised oilseed rape in a mild winter, despite only growing until mid-September when winter wheat was sown. An undersown catch crop of peas, then grown until November, reduced leaching by 15 kg N ha−1. Direct drilling of winter wheat after oilseed rape had no effect. These findings show that there are risks of enhanced leaching in early spring after a cold winter with a snow cover and superficially frozen soil. Optimising the spring N rate for oilseed rape was the most effective measure to decrease leaching in both mild and cold winters, and this effect was improved by an undersown catch crop in a mild winter.

Mulch N recycling in green manure leys under Scandinavian conditions

Nitrogen (N) recycling to the regrowth of mulched red clover (Trifolium pratense L.) and mulched mixed red clover/perennial ryegrass (Lolium perenne L.) leys was determined in field experiments during three consecutive years using 15N-labelled shoot material. Nitrogen recycling was greater in the pure clover stands than in the mixed stands in the beginning of the growing season, but increased successively in the mixed stands so that it was similar (14–15.5%) in both stands at the end of the season. This recycling of N from the mulch led to increased biomass accumulation but did not alter stand composition in the mixed stands. Mulch-derived N was incorporated into the soil organic N in both pure clover and mixed stands which thus contributed to building up soil fertility. An approximately similar proportion of N remained unaccounted for in mulched pure clover and mixed stand leys and presumably represented gaseous losses. To exploit the benefits of green manure leys in the humid temperate zone while minimising the negative environmental impact, these should be harvested rather than mulched.

Effect of wood ash and crushed rock soil amendments on red clover growth and dinitrogen fixation

The fertiliser effect of adding wood ash or crushed rock to a low-fertility soil, compared with an unamended control, was assessed in a pot experiment with a perennial ryegrass-red clover mixture. Dinitrogen (N2) fixation by the clover and translocation of fixed N to the grass were determined using 15N natural abundance. The wood ash produced the highest accumulated clover biomass over two cuts, followed by the crushed rock. Chemical analyses suggested that the increase was due to K supply by the amendments. The wood ash also led to larger amounts of fixed N compared with the control. However, N2 fixation was not increased as much as biomass amount, leading to dilution of plant N. There were minor or no treatment effects on mineralisation from soil N pools. This indicates that good-quality wood ash can be successfully used as a multi-element soil amendment to enhance clover growth on low-fertility soils.