Marie Kummerová

The use of physiological characteristics for comparison of organic compounds phytotoxicity.

The influence of intact (FLT) and photomodified (phFLT) fluoranthene (0.05, 0.5 and 5 micromol l(-1)) and herbicide Basagran (5, 20, 35 and 50 nmol l(-1)) on the germination, growth of seedlings and photosynthetic processes in pea plants (Pisum sativum L., cv. Garde) was investigated. The germination was significantly inhibited already by the lowest concentration (0.05 micromol l(-1)) of FLT and phFLT, while Basagran caused inhibition only in higher concentrations (35 and 50 nmol l(-1)). The growth of roots was significantly inhibited by higher concentration 5 micromol l(-1) of both FLT and phFLT and the shoot of seedlings was significantly influenced only by photomodified form. The length of root and shoot was inhibited already by concentration 5 nmol l(-1) of Basagran. Organic compounds applied on chloroplasts suspension influenced primary photochemical processes of photosynthesis. In chlorophyll fluorescence parameters, the significant increase of F(0) values and the decrease of F(V)/F(M) and Phi(II) values by application of FLT (0.5 and 5 micromol l(-1)) and phFLT (0.05, 0.5 and 5 micromol l(-1)) was recorded. The maximum capacity of PSII (F(V)/F(M)) was influenced by the highest (50 nmol l(-1)) and the effective quantum yield of PSII (Phi(II)) already by the lowest (5 nmol l(-1)) concentration of Basagran. Hill reaction activity decreased and was significantly inhibited by higher concentration (0.5 and 5 micromol l(-1)) of FLT and phFLT and already by the lowest concentration (5 nmol l(-1)) of Basagran.

Principles of classification of medicinal plants as hyperaccumulators or excluders

Strategies of plants, known as metallophytes, in response to metal excess are explored. Specific features of medicinal plants related to metal exposition are discussed. Different parameters used for metallophyte classification are discussed. Bioaccumulation and translocation factors are characterized. Chamomile (Matricaria recutita L.), one of the most important medicinal plants, is presented as a case history. Based on actual knowledge of plant response to metal excess and published data related to chamomile, it has been concluded that this plant species is Cd hyper/accumulator. Thus, chamomile can manifest high potential for cleaning-up (phytoremediation) of the soils contaminated with cadmium. However, it should be stressed that cultivation of this medicinal plant under natural conditions for pharmaceutical use should be carefully supervised.

Effect of zinc and cadmium on physiological and production characteristics in Matricaria recutita

Effects of zinc (12–180 μM) alone and in mixtures with 12 μM Cd on metal accumulation, dry masses of roots and shoots, root respiration rate, variable to maximum fluorescence ratio (FV/FM), and content of photosynthetic pigments were studied in hydroponically cultivated chamomile (Matricaria recutita) plants. The content of Zn in roots and shoots increased with the increasing external Zn concentration and its accumulation in the roots was higher than that in the shoots. While at lower Zn concentrations (12 and 60 μM) the presence of 12 μM Cd decreased Zn accumulation in the roots, treatment with 120 and 180 μM Zn together with 12 μM Cd caused enhancement of Zn content in the root. Presence of Zn (12–120 μM) decreased Cd accumulation in roots. On the other hand, Cd content in the shoots of plants treated with Zn + Cd exceeded that in the plants treated only with 12 μM Cd. Only higher Zn concentrations (120 and 180 μM) and Zn + Cd mixtures negatively influenced dry mass, chlorophyll (Chl) and carotenoid content, FV/FM and root respiration rate. Chl b was reduced to a higher extent than Chl a.

Root response in Pisum sativum and Zea mays under fluoranthene stress: Morphological and anatomical traits

Introduced organic pollutants in all ecosystem compartments can cause stress resulting in a wide range of responses including different root development. In this study, the effects of a polycyclic aromatic hydrocarbon-fluoranthene (FLT; 0.1, 1 and 7 mg L(-1)) on the growth, morphology and anatomical structure of roots of pea and maize was evaluated. In comparison with pea, significant stimulation of root system growth of maize caused by 0.1 mg L(-1) (total length longer by 25%, number of lateral roots by 35%) and its reduction (total length by 34%) already by 1 mg L(-1) FLT is the proof of different interspecies sensitivity to low and higher environmental loading. Nevertheless in both plant species a high loading 7 mg L(-1) FLT significantly reduced both growth (total length by 95% in pea, 94% in maize) and the number of lateral roots (by 78% in pea, 94% in maize). Significantly increased thickness of root of both maize and pea was caused by 7 mg L(-1) FLT and in maize already by 0.1 mg L(-1) FLT. It may be mainly connected with an enlargement of stele area (up to 50% in pea and 25% in maize). Increased xylem area in root tip (by up to 385% in pea, 167% in maize) and zone of maturation (up to 584% in pea, 70% in maize) and its higher portion in stele area of root tip (by 9% in pea, 21% in maize), mainly in roots exposed 7 mg L(-1) FLT, are a proof of an early differentiation of vascular tissue and a shortening of root elongation zone. Moreover in both plant species exposed to this treatment, the decline of rhizodermis cells and external layers of primary cortex was found and also significant deformation of primordia of lateral roots was recorded.

Possible ecological risk of two pharmaceuticals diclofenac and paracetamol demonstrated on a model plant Lemna minor.

Lemna minor is often used in environmental risk assessment and it can be supposed that usually evaluated parameters will be reliable even for assessing the risk of pharmaceuticals. Subtle changes in duckweed plant number, biomass production, and leaf area size induced by 10-day-exposure to diclofenac (DCF) and paracetamol (PCT) (0.1, 10, and 100 μg/L), excepting 100 μg/L DCF, are in contrast with considerable changes on biochemical and histochemical level. Both drugs caused a decrease in content of photosynthetic pigments (by up to 50%), an increase in non-photochemical quenching (by 65%) and decrease in relative chlorophyll fluorescence decay values (by up to 90% with DCF). Both DCF and especially PCT increased amount of reactive nitrogen and oxygen species in roots. DCF-induced effects included mainly increased lipid peroxidation (by 78%), disturbation in membrane integrity and lowering both oxidoreductase and dehydrogenase activities (by 30%). PCT increased the content of soluble proteins and phenolics. Higher concentrations of both DCF and PCT increased the levels of oxidised ascorbate (by 30%) and oxidised thiols (by up to 84% with DCF). Glutathion-reductase activity was elevated by both pharmaceuticals (nearly by 90%), glutathion-S-transferase activity increased mainly with PCT (by 22%). The early and sensitive indicators of DCF and PCT phytotoxicity stress in duckweed are mainly the changes in biochemical processes, connected with activation of defense mechanisms against oxidative stress.

Effect of organic pollutant treatment on the growth of pea and maize seedlings

This study confirmed the considerable effect of polycyclic aromatic hydrocarbon fluoranthene (FLT; 0.01, 0.1, 1, 4 and 7 mg/l) exposure on the germination of seeds, growth and root morphology of seedlings in Zea mays and Pisum sativum. Seed germination was significantly inhibited at FLT≥0.01 mg/l in maize and at ≥1 mg/l in pea. The amount of released ethylene after 3 days of germination was significantly increased in both species at FLT≥0.1 mg/l. After 7 days of seedling cultivation a significant decrease in the dry weight of roots and shoots occurred in maize at FLT≥0.1 mg/l while in pea similar effect was observed at ≥1 mg/l. The total length of primary and lateral roots was significantly reduced by FLT≥1 mg/l in maize and by 4 and 7 mg/l in pea. The length of the non-branched part of the primary root was significantly reduced by FLT≥0.1 mg/l in maize and ≥0.01 mg/l in pea. In both species the number of lateral roots was significantly increased at FLT≤1 mg/l and inhibited at concentrations of 4 and 7 mg/l. Fluoranthene content in roots and shoots of both species positively correlated with the FLT treatment.

Phytotoxicity studies of benzo(a)pyrene with Lactuca sativa.

In this project the effect of increasing concentration of benzo[a] pyrene (0.1, 1, 5, 10, 50 and 100 mg/L), the interaction of sodium humate (10, 100 and 500 mg/L) and BaP (0.1–10 mg/L) and the effect of environmental acidity (pH 4.5, 6.5 and 8.5) on the growth of the root and the hypocotyl of lettuce in the dark and in the light was studied. The results obtained document the fact that increasing BaP concentration (0.1–10 mg/L) stimulated the length of both root and hypocotyl of plants growing in the dark as well as in light. Significant inhibition of growth was demonstrated in plants cultivated in BaP of 100 mg/L concentration. Significantly lower lettuce root and hypocotyl growth in comparison with stimulating effects of BaP was recorded in the application of all combinations of BaP with sodium humate. In neutral and alkaline environments (pH 6.5 and 8.5) no significant effect of pH was found on the action of BaP. In an acid environment (pH 4.5) a synergic effect of environmental acidity and BaP toxicit...

Phytotoxicity of iron in relation to its solubility conditions and the effect of ionic strength

The effect of iron on root elongation and seed germination of Lactuca sativa was investigated within the limits of the plants sensitivity to an acidic environment and iron solubility under experimental conditions. Because ionic strength was found to have phytotoxic effects, the same pH and ionic strength conditions were kept constant over the entire iron concentration range, so as to evaluate solely the effect of iron. 0.14 mM Fe3+ was found to be the lowest effective concentration inhibiting root elongation. The low solubility of iron at a pH of 3 (the lowest value which could be applied for root elongation) excluded the testing of higher iron concentrations. Due to the lower sensitivity of seed germination to acidity, a pH of 2.6 could be applied in this case. The lowest effective iron concentration (the lowest applied concentration causing a significant decrease in germination as compared to the control, P < 0.05), EC50 (the concentration at which germination was 50% of that of the control specimens) and the completely effective iron concentration (the concentration that completely inhibited seed germination) were 0.6, 0.92 and 2.0 mM, respectively.

Photoinduced toxicity of fluoranthene on primary processes of photosynthesis in lichens

The effect of increasing concentrations (0.01, 0.1, 1 and 5 mg l-1) of intact (FLT) and photo-modified (phFLT) fluoranthene and the duration of exposure (1, 2, 3, 5 and 7 days) on the chlorophyll fluorescence parameters (F0, FV/FM, and PhiII) of symbiotic algae in the thalli of two foliose lichens Lasallia pustulata and Umbilicaria hirsuta was investigated. In addition the FLT concentration in thalli of both lichen species was determined and a bioconcentration factor (BCF) was calculated. The results obtained demonstrated that the concentrations of FLT and especially phFLT (1 and 5 mg l-1) applied affected primary photochemical processes of photosynthesis in the algae of both lichen species. The F0 value increased and the FV/FM and PhiII values decreased. The fluoranthene content in the thallus of both lichen species increased with increasing FLT concentration in the environment.

Project Tocoen. The fate of selected organic compounds in the environment. The growth response of maize to increasing concentrations of fluoranthene

Abstract The influence of increasing concentration of fluoranthene (FLT) in the nutrient solution (10, 100 and 1000 μg l‐1) on the growth, the content of assimilation pigments and the rate of photosynthesis of maize plants was studied. Accumulation of FLT in individual leaves and other organs was also measured. As early as after 10 days of cultivation a significant inhibition of the root and shoot growth was found in plants at FLT concentrations of 100 and 1000 μg l ‐1. At 1000 μg l ‐1 significant drop of chlorophyll content a was registered. After 24 days of cultivation, in plants from all treatments a significant inhibition of root and shoot growth was observed and the concentration of photosynthetic pigments was also much lowered. Net photosynthetic rate was significantly inhibited in plants from FLT 100 and FLT 1000 treatments. The highest concentration of FLT was found in roots of the experimental plants from all treatments during the whole period of cultivation. Translocation of some amount of FLT i...