Marija Vučemilo
University of Zagreb
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Featured researches published by Marija Vučemilo.
Arhiv Za Higijenu Rada I Toksikologiju | 2010
Ljerka Prester; Jelena Macan; Kristina Matković; Marija Vučemilo
Determination of Aspergillus Fumigatus Allergen 1 in Poultry Farms Using the Enzyme Immunoassay Poultry farms contain high levels of allergenic fungi, and Aspergillus spp. is the most common genus of moulds. Aspergillus fumigatus antigens are responsible for the development of several respiratory diseases including asthma. The aim of this study was to measure the mass fraction of Asp f 1, a major allergen of Asperillus fumigatus in 37 indoor dust samples collected from four poultry farms in a rural area of the Zagreb County (Croatia) using the enzyme-linked immunosorbent assay. More than 62 % of dust samples had detectable Asp f 1 levels (limit of detection 3.6 ng g-1). The overall mean Asp f 1 level was 17.9 ng g-1 [range (3.8 to 72.4) ng g-1]. Satisfactory results were obtained for analytical within-run imprecision (6.7 %), between-run imprecision (10.5 %), and accuracy (91 % to 115 %). Microclimate parameters (air temperature, relative humidity, and velocity) were within the recommended ranges in all poultry farms. This study has shown that Asp f 1 settles on dust at poultry farms and that occupational exposure to this allergen deserves monitoring in livestock buildings. Određivanje alergena Asp f 1 (Aspergillus fumigatus) u peradarniku enzimimunokemijskom metodom Peradarnici sadržavaju veliku koncentraciju alergenih plijesni, a rod Aspergillus najčešće je zastupljen. Antigeni soja Aspergillus fumigatus odgovorni su za nastanak nekoliko respiratornih bolesti uključujući astmu. Cilj ovoga rada bio je odrediti masenu frakciju Asp f 1, glavnog alergena soja Asperillus fumigatus u 37 uzoraka prašine uzorkovanih u četiri peradarnika sa šireg područja Zagrebačke županije rabeći enzimimunokemijsku metodu. Više od 62 % uzoraka prašine u tri peradarnika imalo je mjerljivu koncentraciju Asp f 1 (granica detekcije = 3.6 ng g-1). Ukupni srednji maseni udio Asp f 1 iznosio je 17.9 ng g-1 (raspon od 3.8 ng g-1 do 72.4 ng g-1). Dobiveni su zadovoljavajući rezultati za analitičku nepreciznost u seriji (6.7 %), nepreciznost iz dana u dan (10.5 %) i točnost (91 % do 115 %). Mikroklimatski parametri (temperatura zraka, relativna vlaga i protok zraka) u svim peradarnicima bili su u okviru preporučenih vrijednosti. Rezultati ovoga rada pokazuju da Asp f 1 sedimentira na prašinu u peradarnicima te da profesionalnu izloženost tom alergenu treba pratiti u jedinicama za uzgoj stoke.
Arhiv Za Higijenu Rada I Toksikologiju | 2009
Kristina Matković; Marija Vučemilo; Bara Vinković
Airborne Fungi in Dwellings for Dairy Cows and Laying Hens The air of animal dwellings can contain great amounts of bioaerosol composed of dust, bacteria, fungi, and endotoxins. The composition may depend on animal species, building construction, animal accommodation, and microclimate parameters, to name just a few factors. Pathogens contained may be a serious threat to animal and human health. The aim of our study was to analyse the fungi aerosol content in a stable housing dairy cows and in a coop for laying hens over the three autumn months of 2007. The air was sampled on Petri dishes with Sabouraud glucose agar. After laboratory treatment, we identified the most common fungi. Their count in the stable ranged from 3.98×103 CFU m-3 to 5.11×104 CFU m-3 and in the coop from 6.89 ×104 CFU m-3 to 1.13×105 CFU m-3. The difference between the two animal dwellings was statistically different at the level of p<0.05. In both dwellings, the most common were the fungi Aspergillus sp., Penicillium sp., and yeasts, followed by Cladosporium sp., Fusarium sp., Mucor sp., Scopulariopsis sp., Alternaria sp., and Rhizopus sp. Our results are entirely in line with values reported in literature and are at the lower end of the range. They call for further investigation that would eventually lead to setting air quality standards for animal dwellings and to developing reliable monitoring systems in order to ensure safe food and safe environment. Gljivice kao sastavni dio bioaerosola u nastambama za muzne krave i nesilice konzumnih jaja U Zraku Nastambi za držanje životinja stvaraju se znatne količine bioaerosola. Njega čine prašina, bakterije, gljivice, endotoksini i plinovi. Brojnost im ovisi o građevinsko-tehničkim značajkama nastambi, naseljenosti životinjama, načinu držanja, temperaturno-vlažnim odnosima u staji i aktivnostima oko hranjenja, mužnje, skupljanja jaja i drugih poslova. Ove čestice, ako su patogene, mogu biti ozbiljna prijetnja za zdravlje ljudi. Mjerenja su obavljana u staji muznih krava te u objektu za nesilice, 2007. godine, tijekom tri jesenja mjeseca. Zrak je uzorkovan na Petrijeve zdjelice sa Sabouraudovim glukoznim agarom, uređajem MAS 100. Nakon obrade u laboratoriju, prema osnovnim i mikromorfološkim osobinama poraslih kolonija identificirani su najčešće zastupljeni rodovi gljivica. Srednja vrijednost broja gljivica u zraku staje za muzne krave kretala se od 3,98×103 CFU m-3 do 5,11×104 CFU m-3. Broj gljivica u zraku objekta za nesilice kretao se od 6,89×104 CFU m-3 do 1,13×105 CFU m-3. Ove vrijednosti statistički su se značajno razlikovale na razini p<0,05. U obje pretraživane nastambe najčešće su bili zastupljeni rodovi Aspergillus, Penicillium i kvasnice. U manjem postotku utvrđene su gljivice iz rodova Cladosporium sp., Fusarium sp., Mucor sp., Scopulariopsis sp., Alternaria sp. i Rhizopus sp. Rezultati ovih istraživanja o kvantitativnom i kvalitativnom sastavu gljivica u zraku pretraženih nastambi potpuno su u skladu s vrijednostima zabilježenim u literaturi te se nalaze na donjim granicama opisanih raspona. Utvrđeni broj i rodovi gljivica ukazuju na nužnost daljnjih istraživanja te potrebu postavljanja standardnih vrijednosti glede kvalitete zraka u nastambama za životinje, kao i razvoj vjerodostojnog sustava praćenja navedenih čimbenika, s ciljem stvaranja sigurne hrane i sigurnog okoliša.
Journal of Occupational and Environmental Medicine | 2014
Anita Ljubičić; Veda Marija Varnai; Marija Vučemilo; Kristina Matković; Dinka Milić; Jelena Macan
Objective:The noninvasive biomarkers of respiratory impairment were assessed in 15 swine confinement (SC) workers and 9 respiratory healthy, nonsmoking volunteers (HV). Methods:Spirometry, fraction of exhaled nitric oxide (FeNO), and exhaled breath condensate (EBC) pH were assessed in SC workers after one working shift and one working week and in HV after 5-hour exposure in SC. Results:Half of the respiratory symptoms (in 8 of 15 SC workers) were work-related. Basal FeNO values were 7.5 ppb higher in the SC workers compared with HV. In the SC workers, EBC pH increased for 0.17 at the end of a working week (P < 0.001). In HV, 5-hour exposure in SC induced 8% drop in forced expiratory flow at 25% of the pulmonary volume (FEF25) (P = 0.008), EBC pH drop for 0.10 units (P = 0.003), and FeNO drop by 1.8 ppb (P = 0.047). Conclusions:EBC pH was suggested as a biomarker of acute airway acidification in HV, whereas the SC workers showed signs of the “healthy worker effect.”
Macedonian veterinary review | 2014
Željko Pavičić; Mario Ostović; Sven Menčik; Anamaria Ekert Kabalin; Marija Vučemilo; Kristina Matković; Boris Antunović; Rajko Pavešić; Vlatko Ilieski
Abstract In the present study, postural behaviour was compared between gilts kept in service unit with different types of flooring during all seasons. The study included four 28-day production cycles and 10 gilts per cycle, equally divided into a control and an experimental group. Control gilts were housed in gestation stalls with slatted concrete floor, whereas in the experimental group the floor was covered with an adjusted rubber mat. Postural behaviour of gilts was observed 4 times per cycle for 4 hours. Study results showed that during cooler seasons, gilts in concrete stalls spent more time standing and lying sternally, whereas gilts in matted stalls were mostly lying, predominantly laterally (P<0.001 all). There were no significant between group differences according to the time the gilts spent sitting or the frequency of changing posture in any season observed. Nevertheless, experimental animals spent significantly less time changing standing to both lying positions during all seasons (P<0.01 all). In conclusion, rubber mats may improve lying comfort in gilts; however, when using rubber mats, the house thermal conditions should be taken in consideration.
Acta Veterinaria-beograd | 2011
Marija Vučemilo; Draženka Gutzmirtl; Marcela Šperanda; Bara Vinković; Emilija Frižon; Kristina Matković; Hrvoje Gutzmirtl
The research was accomplished on a farm with industrial housing of sows and on a family agricultural farm where sows are freely housed, outdoors. Twenty breeding sows from each farm were studied in this research. The experiment was conducted aiming to compare housing of sows, as well as those factors affecting stress indicators. For this purpose cortisol level was determined five days before farrowing, five days after farrowing and a day after weaning. Besides cortisol, other haematological parameters were determined in the blood, also. Statistically significant higher concentration of cortisol was determined at five days before farrowing in sows housed freely compared to sows housed industrially (p<0.05). Five days after farrowing and a day after weaning, concentrations of cortisol were lower in the blood of freely housed sows compared to industrially housed sows. However, the difference was not significant. The level of cortisol determined in sows housed freely during pregnancy, and after dislocation into wedged farrowing departments, five days before farrowing, was significantly higher than in industrial sows. The aforesaid confirmed stress that can be induced by relocating the sows to a new location and wedged space. The level of cortisol, determined in industrial sows was almost of equal values during the whole research, the fact being related to constant stress conditions.
Veterinarski Arhiv | 2017
Kristina Matković; Ljerka Prester; Tatjana Orct; Jelena Macan; Veda Marija Varnai; Danijel Marušić; Mario Ostović; Željko Pavičić; Marija Vučemilo
In the present study, air quality in a cage-housed laying hen house was investigated throughout the seasons by assessing dust and endotoxin concentrations. Measurements were done twice a month during the 1-year production cycle. The mean levels of dust and endotoxins ranged from 0.60 mg/m3 in May to 2.83 mg/m3 in November, and from 203.15 EU/m3 in August to 745.53 EU/m3 in April. Significantly higher concentrations of dust and endotoxins in the poultry house were determined in the autumn and winter seasons, and endotoxins in the spring season as well, compared to the summer (P<0.05 all). The results suggested that the cooler periods of the year pose a greater risk for the welfare and performance of laying hens, but also for the health of humans working in these settings in terms of the levels of airborne dust and endotoxins.
International Archives of Occupational and Environmental Health | 2010
Davor Rimac; Jelena Macan; Veda Marija Varnai; Marija Vučemilo; Kristina Matković; Ljerka Prester; Tatjana Orct; Ivančica Trošić; Ivan Pavičić
Czech Journal of Animal Science, Zivocisna vyroba | 2007
Marija Vučemilo; Kristina Matković; Bara Vinković; Slavica Jakšić; Kornelija Granić; Nora Mas
Czech Journal of Animal Science | 2018
Marija Vučemilo; Kristina Matković; Bara Vinković; Jelena Macan; Veda Marija Varnai; Ljerka Prester; Kornelija Granić; Tatjana Orct
Zeitschrift Fur Jagdwissenschaft | 2002
Slavica Jakšić; Sunčica Uhitil; Marija Vučemilo