Mario Ostović

Teat sanitation in lactating sows.

Preweaning piglet mortality is a serious source of economic losses in pig production, with enteric diseases as the most common infectious cause of mortality. The aim of this study was to determine the efficacy of teat sanitation in lactating sows in order to reduce the piglet bacterial invasion through contaminated teats leading to outbreaks of enteric diseases. The study was carried out in a farrowing pen with 60 sows divided into three groups of 20 animals. Group 1 animals served as control group and were left untreated. In group 2, sow teats were treated with water and in group 3 with a commercial antiseptic with potent bactericidal action. Microbiological purity of the teats was determined during 24 days of the piglet suckling period. Study results showed the bacterial count per teat to be significantly lower in both experimental groups as compared with control group (P<0.01). Also, bacterial count was significantly lower in group 3 treated with the antiseptic than in group 2 treated with water alone (P<0.01). Accordingly, sow teat sanitation as a potential prophylactic measure during the piglet suckling period significantly decreased bacterial count on sow teats, thus reducing the risk of bacterial invasion of the piglet alimentary system.

Attitudes of veterinary students in Croatia toward farm animal welfare

This survey was undertaken to assess the attitudes of Croatian veterinary students regarding farm animal welfare issues. The study included students of all undergraduate years at the only Faculty of Veterinary Medicine in Croatia. First-year students were surveyed twice, ie before and after attending the course on animal welfare, using a written questionnaire containing statements with a five-point Likert scale for choice of answers. Students consider good animal welfare necessary for sustainability of farming systems and food quality and safety, ranking particular issues in the following order: biological functioning > natural living > emotional states. Students also believe that cattle and pigs have greater cognitive abilities and that their welfare is less compromised in comparison with poultry, whereas standard management procedures performed in pig production are perceived as more humane than beak-trimming in poultry. In addition, students tend to consider pain in farm animals caused by management procedures only when these procedures involved the affliction of significant pain. There were no differences between attitude scores on most of the statements from first-year students before and after the course. Furthermore, for the majority of statements, the mean responses were lower in final-year students, suggesting a lower level of empathy toward farm animals. Although similar results have also been recorded elsewhere in the world, these results raise concerns as to the ability of these future veterinarians to promote good farm animal welfare in the country and abroad. The results also suggest a need to modify veterinary student education in Croatia in the field of farm animal welfare.

INFLUENCE OF BREED, GENDER, REPRODUCTIVE STATUS AND ORIGIN ON NOISE RELATED FEARS IN THE BELGRADE POPULATION OF DOGS

The prevalence of canine noise-related fears was studied by the questionnaire method of dogs’ owners from different part of Belgrade. Dog-owners (N=147) were chosen from a variety of sources (permissive municipal areas and parks for walking of dogs, dog shows and exhibitions and veterinary surgeries for small animal practice). Owners indicated noise-related fears in 59 dogs (40.14%). Twenty-six (17.69%) of them were mixed breed dogs and 33 (22.45%) were pure breed dogs. Noise-related fears were indicated in 25 females (17.01%) and in 34 (23.13%) males and, in 27 (18.37%) intact and 32 (21.77%) neutered dogs. From 59 dogs with noise-related fears 13 of them (8.84%) were directly purchased from breeders and 46 (31.30%) were obtained from other sources. There was a statistically significant difference (p<0.001) in the rate of noise-related fears between the dogs directly purchased from breeders and dogs obtained from other sources.

Postural behaviour in gilts housed on concrete and rubber slats during four seasons

Abstract In the present study, postural behaviour was compared between gilts kept in service unit with different types of flooring during all seasons. The study included four 28-day production cycles and 10 gilts per cycle, equally divided into a control and an experimental group. Control gilts were housed in gestation stalls with slatted concrete floor, whereas in the experimental group the floor was covered with an adjusted rubber mat. Postural behaviour of gilts was observed 4 times per cycle for 4 hours. Study results showed that during cooler seasons, gilts in concrete stalls spent more time standing and lying sternally, whereas gilts in matted stalls were mostly lying, predominantly laterally (P<0.001 all). There were no significant between group differences according to the time the gilts spent sitting or the frequency of changing posture in any season observed. Nevertheless, experimental animals spent significantly less time changing standing to both lying positions during all seasons (P<0.01 all). In conclusion, rubber mats may improve lying comfort in gilts; however, when using rubber mats, the house thermal conditions should be taken in consideration.

Influence of meat type, sex and storage time on fatty acid profile of free range dalmatian turkey

Abstract Dalmatian turkey is a slow growing breed kept in free range systems. It is a type of “old fashioned poultry” whose meat is present on the market and accepted by consumers. However, no information about its meat quality and fatty acid profile is available. The chemical composition of the meat was influenced by gender and meat type and these differences could be important from the consumer’s point of view. Fatty acid composition was characterized by the predominance of n6 fatty acids, especially C18:2n6 and a high n6/n3 ratio. Increased time of storage strongly reduced the long chain polyunsaturated fatty acid (LC PUFA) and increased atherogenicity and thrombogenicity indices (AI and TI) in thigh tissue. The content of beneficial n3 PUFA was influenced by meat type, with lower values of C18:3n3 and higher values of LC PUFA in the breast compared to the thighs. The potential intake of LC PUFA of comercial turkey in the human diet was lower in comparison to poultry fed with complete feed mixtures. An interesting fact was the higher DHA values in comparison with DPA values in breast tissue, which is characteristic of old poultry breeds. The Dalmatian turkey is a highly valued traditional product and an important archaic breed for gene preservation and biodiversity. Nevertheless, Dalmatian turkey meat could be even further improved by minimal dietary manipulation to become a product with additional health promoting effects.

PRLR-AluI Gene Polymorphism And Litter Size Traits In Highly Prolific Line Of Topigs 20 Sows

Abstract The objective of the present study was to identify the Prolactin Receptor (PRLR) gene polymorphism related to litter size traits. The study included 101 Topigs 20 line of sows with 426 litters. The traits studied were: Total Number of Born (TNB), Number of Born Alive (NBA), Number of Still Born (NSB), and Number of MUMmified (NMUM) piglets. Polymorphism was identified with the polymerase chain reaction-restriction fragment length polymorphism method. Allelic and genotype frequencies and deviation from Hardy-Weinberg equilibrium were verified with the chi-square test. Analysis of litter size traits was performed using the General Linear Model, which included the potential environmental effects. Additive and dominant allele variances were observed by the regression procedure. In the studied population of sows, the frequency of heterozygotes (0.5149) for PRLR gene exceeded the total number of AA (0.0198) and BB (0.4653) homozygotes, which resulted in a high proportion of B allele (0.7228). The results for PRLR showed statistically significant (P<0.05) differences in first parity sows between BB and AB genotypes for TNB and NBA. Significant differences (P<0.05) were recorded in third parity sows between BB and AB genotypes for NBA, and in AA genotype versus AB and BB genotypes for NMUM. The fourth and subsequent parity sows of AA genotype had a significantly higher (P<0.05) rate of NBA as compared with those of AB and BB genotypes. In all parities analysed, the difference between the BB and AB genotypes for NBA was statistically significant (P<0.05). Interpretation of the results at the levels of phenotypes and either additive or dominant variance was quite difficult due to the small number of AA homozygous sows. The calculation model yielded a significant effect (P<0.05) as well as tendency (P<0.1) for the mentioned effects except for age at first farrowing.

Airborne dust distribution in a farrowing pen in dependence of other microclimatic parameters during spring-summer period

Abstract The study was carried out in a farrowing pen during 6 production cycles of 24 days in the spring-summer period. The effect of season and other microclimate parameters on airborne dust concentration was investigated. Study results showed a decrease in airborne dust concentration in summer period that yielded negative correlation with air temperature (P<0.05). Relative humidity was expected to decrease due to higher ventilation rates, however, a significant increase was recorded in summer (P<0.05) because of higher evaporation caused by floor wash. The decrease in airborne dust concentration was ascribed to temperature elevation due to intensified ventilation rate and relative humidity resulting in higher rate of dust settling.

Dietary supplementation with mannan oligosaccharide and clinoptilolite modulates innate and adaptive immune parameters of weaned pigs

The aim of this study was to assess modulatory effects of dietary supplements mannan oligosaccharide (MOS) and clinoptilolite (CPL) as potential alternatives to antibiotic growth promoters (AGP) given to 4-week old pigs at weaning (Day 0) on their innate/adaptive immunity by determining: alterations in C-reactive protein (CRP) and haptoglobin (HpG) serum levels, efficiency of blood monocytes (MO) and neutrophilic granulocytes (GR) for in vitro phagocytosis (PHC)/microbicidity (MBC) and proportion of extrathymic double positive CD4 CD8 (CD4+CD8+) T cells throughout 35 days of the study. Neither MOS nor CPL changed the serum concentrations of CRP, whereas that of HpG was significantly increased in the CPL supplemented pigs (p<0.05) at Day 35. Activity of PHA of GR was significantly increased by both dietary supplements (p<0.05) from Day 7 to Day 35. Also, the GR from pigs fed with both supplements had significantly increased MBC at Day 7 (p<0.05), but at Day 35 such an increase was observed only for CPL. The in vitro PHC/MBC of MO did not change in either group of supplemented pigs. The pigs supplemented with MOS had a significantly higher proportion of CD4+CD8+ T lymphocytes at Day 28 (p<0.05). Although both supplements showed a promising ability to stimulate rather innate than adaptive cellular immunity, it does not appear that any solely applied natural substance such as MOS or CPL in the current study could be a competitive alternative to conventional AGP for improving health and promoting growth in weaned pigs.

The seasonal influence on airborne dust and endotoxin concentrations in a laying hen house

In the present study, air quality in a cage-housed laying hen house was investigated throughout the seasons by assessing dust and endotoxin concentrations. Measurements were done twice a month during the 1-year production cycle. The mean levels of dust and endotoxins ranged from 0.60 mg/m3 in May to 2.83 mg/m3 in November, and from 203.15 EU/m3 in August to 745.53 EU/m3 in April. Significantly higher concentrations of dust and endotoxins in the poultry house were determined in the autumn and winter seasons, and endotoxins in the spring season as well, compared to the summer (P<0.05 all). The results suggested that the cooler periods of the year pose a greater risk for the welfare and performance of laying hens, but also for the health of humans working in these settings in terms of the levels of airborne dust and endotoxins.

Influence of socio-demographic and experiential factors on the attitudes of Croatian veterinary students towards farm animal welfare

The aim of this study was to examine the factors influencing the attitudes of veterinary students in Croatia toward farm animal welfare. The survey was carried out at the only faculty of veterinary medicine in Croatia and included students from all years of the integrated undergraduate and graduate study programme. The response rate was 91% (n = 505 subjects). According to the socio-demographic and experiential factors observed, females, students in their initial years of study, younger students with urban backgrounds, non-religious/non-spiritual students and vegetarians, and those intending to work with companion animals expressed more concerned attitudes regarding farm animal welfare (all P < 0.05). The type of secondary school completed, family monthly income and previous experience with farm or pet animals had no impact on student attitudes. The results of the study point to a variety of factors influencing veterinary student attitudes towards farm animal welfare. A recognition of these factors can contribute considerably to increasing student awareness of farm animal welfare. These results provide a basis for further research into the factors influencing veterinary attitudes to animal welfare and the complexity of their interactions.