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Featured researches published by Mariko Harada.


Diabetes Research and Clinical Practice | 1992

Hyperlipidemia and atherosclerosis in experimental insulinopenic diabetic monkeys.

Yutaka Harano; Hideto Kojima; Keisuke Kosugi; Masaaki Suzuki; Mariko Harada; Takamitsu Nakano; Hideki Hidaka; Atsunori Kashiwagi; R. Torii; Yuzo Taniguchi; T. Nishimori; Y. Yasuda; Yukio Shigeta

Chronic insulinopenic diabetes was induced by i.v. streptozotocin in the non-human primate Macaca fuscata. Five diabetic monkeys were kept for 8-19 months and nine for 24-48 months without any insulin treatment. Hyperglycemia (241 +/- 22 mg/dl, M +/- SE less than or equal to 1 year) progressed to 376 +/- 34 mg/dl (greater than 2 years) and ketosis to 3.5 mM (greater than 2 years) during the course of diabetes; this was roughly inversely proportional to hypoinsulinemia (3.4 microU/ml, 2 years). Serum cholesterol increased from 184 +/- 11 (less than or equal to 1 year) to 328 +/- 66 mg/dl (greater than 2 years) with the major increase in LDL-cholesterol (2.7-fold over control, greater than 2 years). HDL-cholesterol did not change at all throughout the experimental period. TG increased from 144 +/- 25 (less than or equal to 1 year) to 676 +/- 116 (greater than 2 years) with a major increase in the VLDL fraction (15-fold over control, greater than 2 years). Serum levels of apo B increased to 141 +/- 16 (less than or equal to 2 years) and 223 +/- 8 mg/dl (greater than 2 years) in contrast to control, 73 +/- 2. Morphologically, lipid deposition in the intima and fatty streaks have been observed in the abdominal aorta of all the diabetic monkeys with duration of more than 2 years. In six of the diabetic monkeys atheromatous changes such as intimal and medial thickening with smooth muscle cell proliferation were observed with foam cell formation. Similar atherosclerotic lesions were observed in renal and coronary arteries in at least six of these monkeys. In diabetic monkeys with duration of less than 2 years, mild atherosclerotic lesions were observed in two out of five. The results indicate that long standing insulinopenia leads to metabolic derangements characterized by hyperglycemia, ketonemia and hyperlipidemia. Elevation of LDL-cholesterol and VLDL TG with an increase of apo B is a characteristic of lipoprotein disorder. Morphologically, early to moderately advanced lesions of atherosclerosis were observed in aorta, renal and coronary arteries as a result of metabolic derangement due to insulin deficiency.


Clinica Chimica Acta | 1985

Direct automated assay method for serum or urine levels of ketone bodies

Yutaka Harano; M. Ohtsuki; M. Ida; Hideto Kojima; Mariko Harada; T. Okanishi; Atsunori Kashiwagi; Yukio Ochi; S. Uno; Yukio Shigeta


Japanese Journal of Medicine | 1987

Computerized glucose clamp method for the determination of insulin sensitivity in diabetic subjects.

Mariko Harada; Yutaka Harano; Hideto Kojima; Masaaki Suzuki; Shunichi Furusawa; Yoshihiko Nishino; Takatoshi Uchigaki; Hiroshi Hyodo; Yukio Shigeta


Journal of Clinical Biochemistry and Nutrition | 1988

Abnormalities in the Membrane of Erythrocytes from Two Patients Homozygous for Familial Hypobetalipoproteinemia

Akira Tamura; Keiji Kanda; Tatsuzo Fujii; Yutaka Harano; Mariko Harada; Takamitsu Nakano; Hideki Hidaka; Yukio Shigeta; Teruyasu Suzuki; Tsuyoshi Ohtaka


The journal of Japan Atherosclerosis Society | 1988

Study of Acetyl Low Density Lipoprotein and β-Very Low Density Lipoprotein Metabolism in Macrophage Cell Lines

Hideki Hidaka; Yuzuru Nakazima; Mariko Harada; Takamitsu Nakano; Takahiko Aoki; Keisuke Kosugi; Yutaka Harano; Yukio Shigeta


The journal of Japan Atherosclerosis Society | 1988

Biochemical Analyses of the Erythrocytes and Plasma and Morphological Observations on the Erythrocytes from Homozygous and Heterozygous Cases of a Hypobetalipoproteinemia Family

Akira Tamura; Nobumitsu Tominaga; Tatsuzou Fujii; Yutaka Harano; Mariko Harada; Takamitsu Nakano; Hideki Hidaka; Yukio Shigeta; Teruyasu Suzuki; Takeshi Ohtaka


The journal of Japan Atherosclerosis Society | 1987

LDL Metabolism in Human Peritoneal Macrophages Comparison with those in Cells of Human Origin

Hideki Hidaka; Mariko Harada; Takamitsu Nakano; Yutaka Harano; Yukio Shigeta


The journal of Japan Atherosclerosis Society | 1987

Apoprotein B Deficiency and its Clinical Feature in Homozygous Hypoproteinemia

Yutaka Harano; Hideto Kojima; Mariko Harada; Takamitsu Nakano; Hiroshi Kido; Tsuyoshi Ohtaka; Yukio Shigeta


Diabetes Research and Clinical Practice | 1987

Hyperbetalipoproteinemia as a risk factor for the diabetic macroangiopathy

Yutaka Harano; Hideki Hidaka; Takamitsu Nakano; Mariko Harada; Hideto Kojima; Yukio Shigeta


The journal of Japan Atherosclerosis Society | 1986

Changes of Lipoprotein Levels during Insulin Infusion in Familial Hypercholesterolemia IIa

Mariko Harada; Hideto Kojima; Yoshihiko Nishio; Hideki Hidaka; Takamitsu Nakano; Atsunori Kashiwagi; Yutaka Harano; Yukio Shigeta

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Yukio Shigeta

Shiga University of Medical Science

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Hideki Hidaka

Shiga University of Medical Science

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Takamitsu Nakano

Shiga University of Medical Science

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Hideto Kojima

Shiga University of Medical Science

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Atsunori Kashiwagi

Shiga University of Medical Science

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Masaaki Suzuki

Shiga University of Medical Science

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Akira Tamura

Kyoto Pharmaceutical University

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Teruyasu Suzuki

Shiga University of Medical Science

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