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Metabolism-clinical and Experimental | 1983

Mechanism of adrenergic stimulation of hepatic ketogenesis

Keisuke Kosugi; Yutaka Harano; Takamitsu Nakano; Masaaki Suzuki; Atsunori Kashiwagi; Yukio Shigeta

The effects of alpha- and beta-adrenergic stimulation on ketogenesis were examined in freshly isolated rat hepatocytes in order to determine which alpha- or beta-adrenergic stimulation is involved in the enhancement of ketogenesis. In the presence of 0.3 mmol/L (U-14C)-palmitate, epinephrine, norepinephrine, and phenylephrine at 500 ng/mL increased ketogenesis by 25% (16.0 +/- 0.17 v 12.8 +/- 0.13 nmol/mg protein per hour), 20% (15.3 +/- 0.28) and 20% (15.4 +/- 0.36), respectively. However, isoproterenol even at 1 microgram/mL did not stimulate ketogenesis. Phentolamine (5 micrograms/mL) almost completely abolished the effect of epinephrine on ketogenesis (13.7 +/- 0.30 v 16.0 +/- 0.17) but propranolol did not inhibit the stimulation by epinephrine (15.6 +/- 0.38 v 16.0 +/- 0.17). Trifluoperazine (10 mumol/L), presumably an inhibitor of calcium-dependent protein kinase, abolished the effect of epinephrine (13.6 +/- 0.22 v 16.0 +/- 0.17). These results indicate that catecholamines increase ketogenesis predominantly through the alpha-adrenergic system independent of cyclic AMP, and calcium-dependent protein kinase is thought to be involved in the activation of ketogenesis. On the other hand, glucagon stimulated ketogenesis with an increase of cyclic AMP, which was not inhibited by alpha- and beta-adrenergic antagonists. Alpha-adrenergic stimulation increased hepatic glycogenolysis much more at much lower concentrations when compared with ketogenesis. Stimulation of ketogenesis by catecholamines seemed to be less sensitive and responsive compared with hepatic glycogenolysis.


Diabetes Research and Clinical Practice | 1992

Hyperlipidemia and atherosclerosis in experimental insulinopenic diabetic monkeys.

Yutaka Harano; Hideto Kojima; Keisuke Kosugi; Masaaki Suzuki; Mariko Harada; Takamitsu Nakano; Hideki Hidaka; Atsunori Kashiwagi; R. Torii; Yuzo Taniguchi; T. Nishimori; Y. Yasuda; Yukio Shigeta

Chronic insulinopenic diabetes was induced by i.v. streptozotocin in the non-human primate Macaca fuscata. Five diabetic monkeys were kept for 8-19 months and nine for 24-48 months without any insulin treatment. Hyperglycemia (241 +/- 22 mg/dl, M +/- SE less than or equal to 1 year) progressed to 376 +/- 34 mg/dl (greater than 2 years) and ketosis to 3.5 mM (greater than 2 years) during the course of diabetes; this was roughly inversely proportional to hypoinsulinemia (3.4 microU/ml, 2 years). Serum cholesterol increased from 184 +/- 11 (less than or equal to 1 year) to 328 +/- 66 mg/dl (greater than 2 years) with the major increase in LDL-cholesterol (2.7-fold over control, greater than 2 years). HDL-cholesterol did not change at all throughout the experimental period. TG increased from 144 +/- 25 (less than or equal to 1 year) to 676 +/- 116 (greater than 2 years) with a major increase in the VLDL fraction (15-fold over control, greater than 2 years). Serum levels of apo B increased to 141 +/- 16 (less than or equal to 2 years) and 223 +/- 8 mg/dl (greater than 2 years) in contrast to control, 73 +/- 2. Morphologically, lipid deposition in the intima and fatty streaks have been observed in the abdominal aorta of all the diabetic monkeys with duration of more than 2 years. In six of the diabetic monkeys atheromatous changes such as intimal and medial thickening with smooth muscle cell proliferation were observed with foam cell formation. Similar atherosclerotic lesions were observed in renal and coronary arteries in at least six of these monkeys. In diabetic monkeys with duration of less than 2 years, mild atherosclerotic lesions were observed in two out of five. The results indicate that long standing insulinopenia leads to metabolic derangements characterized by hyperglycemia, ketonemia and hyperlipidemia. Elevation of LDL-cholesterol and VLDL TG with an increase of apo B is a characteristic of lipoprotein disorder. Morphologically, early to moderately advanced lesions of atherosclerosis were observed in aorta, renal and coronary arteries as a result of metabolic derangement due to insulin deficiency.


Clinica Chimica Acta | 1990

Apolipoprotein B-48 analysis by high-performance liquid chromatography in VLDL : a sensitive and rapid method

Hideki Hidaka; Hideto Kojima; Yuzuru Nakajima; Takahiko Aoki; Takaaki Nakamura; Toshihiro Kawabata; Takamitsu Nakano; Yutaka Harano; Yukio Shigeta

Apolipoprotein (apo) B subspecies were separated by high-performance liquid chromatography (HPLC) to analyze Apo B-48 contents in very-low-density lipoproteins (VLDL) more easily. Apo B-100 and B-48 were eluted through two connected column of Shim-pack Diol-300 at a retention time of 24 min and 32.3 min, respectively. The molecular masses estimated by this method were approximately 600 kDa in apo B-100, 220 kDa in apo B-48. % apo B-48 in total apo B (% B-48) determined by measuring peak area of HPLC-separated apo B in a healthy subject was 76% in chylomicrons, 13% in fasting VLDL, and 20% in 2 h-postprandial VLDL. No peak of apo B-48 was detected in LDL. Recovery of Apo B determined by re-chromatography of HPLC-separated sample was 91 +/- 4.0% and 95 +/- 3.6% in Apo B-100 and apo B-48, respectively. 125I-labeled apo B in VLDL were also analyzed by both HPLC and SDS-PAGE. Percent radioactivity of apo B-48 fraction in the total apo B determined by the HPLC (17.3%) was similar to the value obtained through the measurement of radioactivity separated by the SDS-PAGE (17.6%). Coefficient of variation in % B-48 determined by the peak area was 2.5%. Percent B-48 determined by the HPLC method was significantly correlated with % B-48 by SDS-PAGE, but 6-8 times higher, which might be accounted for in part by the reported difference of chromogenicity between apo B-100 and apo B-48. % B-48 in VLDL separated from fasting plasma were 17.1 +/- 5.6% in 14 healthy subjects, and positively related to VLDL triglyceride and cholesterol concentrations. Apo B isoprotein analysis by the HPLC method is reliable and easy to perform for studying apo B metabolism in triglyceride-rich lipoproteins in physiological as well as pathological conditions.


FEBS Letters | 1986

A suppressive role of c-kinase for the stimulation of hepatic ketogenesis by glucagon and epinephrine

Hideto Kojima; Yutaka Harano; Keisuke Kosugi; Takamitsu Nakano; Yukio Shigeta

Long‐chain fatty acid oxidation Ketogenesis c‐Kinase Glucagon Epinephrine Carnitine palmitoyltransferase


Diabetes Research and Clinical Practice | 1991

In vitro stimulation of glucose utilization by insulin in primary cultures of rat hepatocytes

Masaaki Suzuki; Hideto Kojima; Takamitsu Nakano; Atsunori Kashiwagi; Hideki Hidaka; Keisuke Kosugi; Yukio Shigeta; Yutaka Harano

The effect of glucose concentration and insulin on glucose incorporation was studied in primary cultures of rat hepatocytes. The rate of glucose incorporation into hepatocytes was proportional to the medium glucose concentration from 100 to 800 mg/dl. At 800 mg/dl glucose the rate reached a plateau. Of the glucose taken up by hepatocytes, 16 and 18% was incorporated into glycogen and lipid, respectively, and 58% into the nucleotide fraction after incubation for 4 h. In the medium, lactate was the major product found. Insulin stimulates glucose incorporation by 20-112% into all the above pathways at glucose concentrations between 100 and 800 mg/dl. The insulin effect was noted as early as 2-4 h (early effect) and up to 24 h (delayed effect). This effect of insulin was observed to be dose dependent from 5 to 200 ng/ml insulin. While the delayed insulin effect was abolished by cycloheximide, the early effect of insulin was not affected. With respect to the key enzyme activities of glucose utilization, activation of glycogen synthase (increase of I-activity/total activity) and pyruvate kinase (activation at 0.2 mM phosphoenolpyruvate) was noted 4 h after insulin addition, and these effects were not abolished by cycloheximide. These two enzymes increased in total activity after 24 h. Both glucokinase and glucose-6-phosphate dehydrogenase activities increased by 30-35% and 65-93% at 4 and 24 h, respectively. The results indicate that hepatocytes directly utilize glucose in a dose-dependent manner with respect to glucose and insulin. A major early and delayed effect of insulin appeared due to the activation and induction of the key hepatic enzymes of glucose utilization, respectively.


Endocrine Journal | 2006

Development of Cookie Test for the Simultaneous Determination of Glucose Intolerance, Hyperinsulinemia, Insulin Resistance and Postprandial Dyslipidemia

Yutaka Harano; Takeshi Miyawaki; Junko Nabiki; Miki Shibachi; Tomomi Adachi; Michiko Ikeda; Fukuhiro Ueda; Takamitsu Nakano


Archive | 1984

Reagents for enzyme immunoassay for apolipoprotein B

Yukio Shigeta; Yutaka Harano; Takamitsu Nakano; Hideo Nishikawa


Journal of Clinical Biochemistry and Nutrition | 1988

Abnormalities in the Membrane of Erythrocytes from Two Patients Homozygous for Familial Hypobetalipoproteinemia

Akira Tamura; Keiji Kanda; Tatsuzo Fujii; Yutaka Harano; Mariko Harada; Takamitsu Nakano; Hideki Hidaka; Yukio Shigeta; Teruyasu Suzuki; Tsuyoshi Ohtaka


Biomedical Research-tokyo | 1980

A new somatostatin analog, des(Ala 1 . Gly 2 )[D-Trp 8 , D-Asu 3-14 }-somatostatin with relative specificity for glucagon suppression

Yutaka Harano; Hideki Hidaka; Takamitsu Nakano; Junji Emura; Terutoshi Kimura; Syunpei Sakakibara; Yukio Shigeta


The journal of Japan Atherosclerosis Society | 1986

Enzyme Immunoassay of Apo B and its Clinical Significance

Yutaka Harano; Takamitsu Nakano; Hideto Kojima; Yasuo Kida; Yoshihiro Kuriyama; Toru Sawada; Sugao Fukui; Yukio Shigeta

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Yukio Shigeta

Shiga University of Medical Science

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Hideki Hidaka

Shiga University of Medical Science

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Hideto Kojima

Shiga University of Medical Science

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Mariko Harada

Shiga University of Medical Science

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Atsunori Kashiwagi

Shiga University of Medical Science

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Masaaki Suzuki

Shiga University of Medical Science

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Takahiko Aoki

Shiga University of Medical Science

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Akira Tamura

Kyoto Pharmaceutical University

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