Mark J. VanGessel

Glyphosate-resistant horseweed from Delaware

Abstract No-tillage corn (Zea mays L.) and soybean [Glycine max (L.) Merr.] production has been widely accepted in the mid-Atlantic region, favoring establishment of horseweed [Conyza canadensis (L.) Cronq.]. Within 3 yr of using only glyphosate for weed control in continuous glyphosate-resistant soybeans, glyphosate failed to control horseweed in some fields. Seedlings originating from seed of one population collected in Delaware were grown in the greenhouse and exhibited 8- to 13-fold glyphosate resistance compared with a susceptible population. There were no differences between the isopropylamine or diammonium salts of glyphosate. Nomenclature: Corn; Zea mays L.; horseweed; Conyza canadensis (L.) Cronq. ERICA; soybean; Glycine max (L.) Merr.; glyphosate.

Predicting weed emergence for eight annual species in the northeastern United States

Abstract A 2-yr experiment assessed the potential for using soil degree days (DD) to predict cumulative weed emergence. Emerged weeds, by species, were monitored every 2 wk in undisturbed plots. Soil DD were calculated at each location using a base temperature of 9 C. Weed emergence was fit with logistic regression for common ragweed, common lambsquarters, velvetleaf, giant foxtail, yellow foxtail, large crabgrass, smooth pigweed, and eastern black nightshade. Coefficients of determination for the logistic models fit to the field data ranged between 0.90 and 0.95 for the eight weed species. Common ragweed and common lambsquarters were among the earliest species to emerge, reaching 10% emergence before 150 DD. Velvetleaf, giant foxtail, and yellow foxtail were next, completing 10% emergence by 180 DD. The last weeds to emerge were large crabgrass, smooth pigweed, and eastern black nightshade, which emerged after 280 DD. The developed models were verified by predicting cumulative weed emergence in adjacent plots. The coefficients of determination for the model verification plots ranged from 0.66 to 0.99 and averaged 0.90 across all eight weed species. These results suggest that soil DD are good predictors for weed emergence. Forecasting weed emergence will help growers make better crop and weed management decisions. Nomenclature: Common lambsquarters, Chenopodium album L. CHEAL; common ragweed, Ambrosia artemisiifolia L. AMBEL; eastern black nightshade, Solanum ptycanthum Dun. SOLPT; giant foxtail, Setaria faberi Herrm. SETFA; large crabgrass, Digitaria sanguinalis (L.) Scop. DIGSA; smooth pigweed, Amaranthus hybridus L. AMACH; velvetleaf, Abutilon theophrasti Medikus ABUTH; yellow foxtail, Setaria glauca (L.) Beauv. SETLU.

Horseweed (Conyza canadensis) seed collected in the planetary boundary layer

Abstract Horseweed is a winter or summer annual plant, native to North America and distributed worldwide in temperate climates. This plant is considered an important agricultural weed because it can reduce agricultural yields by 90% at high densities and becomes problematic under low-tillage agriculture. Seed production is robust with an estimated 200,000 seeds produced per plant, and seed dispersal is wind-assisted. The confirmation of glyphosate-resistant horseweed in Delaware in 2001 and the rapid spread of the resistant biotype, currently covering more than 44,000 ha, has necessitated a change in the discussion about weed dispersal. Large radio-controlled airplanes were used to sample the lower atmosphere for the presence of horseweed seeds during a 3-d period in early September 2005 in southern Delaware. The collection of multiple seeds at heights ranging from 41 to 140 m above ground level strongly suggests that horseweed seeds are entering the Planetary Boundary Layer (PBL) of the atmosphere, where long-ranged transport of aerial biota frequently occurs. With wind speeds in the PBL frequently exceeding 20 m s−1, seed dispersal can easily exceed 500 km in a single dispersal event. Nomenclature: Horseweed, Conyza canadensis (L.) Cronq.

Glyphosate in Double-Crop No-Till Glyphosate-Resistant Soybean: Role of Preplant Applications and Residual Herbicides1

The role of preplant glyphosate applications and residual herbicides in the efficacy of glyphosate for weed management in double-crop no-till glyphosate-resistant soybean (GRS) was investigated in the coastal plains of Mid-Atlantic United States. The experiment had a two- by two- by five-factorial treatment structure laid out in three or four randomized complete blocks at research centers in Delaware and New Jersey. The factors investigated were preplant weed management: preplant or no preplant glyphosate applications; postemergence (POST) herbicide treatments: 0.8 kg ae/ha glyphosate alone or 0.8 kg/ha glyphosate tank-mixed with 0.6 kg ai/ha clomazone plus 0.07 kg ai/ha imazethapyr; and GRS growth stage at herbicide application which ranged from cracking, 5 to 8 d after planting, (DAP) to the V6 stage (35 DAP). Preplant glyphosate applications did not influence the efficacy of POST glyphosate applications alone or with the residual herbicides. Glyphosate alone or with clomazone plus imazethapyr provided excellent control of horseweed and fall panicum irrespective of the time of herbicide application from GRS at cracking to the V6 stage. With other weed species, residual herbicide influence varied with year, weed species, and GRS growth stage at herbicide application. Generally, glyphosate alone was most effective when applied at the V2 to V6 stages (16 to 35 DAP). A tank-mix of glyphosate with clomazone plus imazethapyr extended this window to include applications at GRS cracking and the V1 stage. Herbicide treatments were safe on GRS at all stages of application up to the V6 stage (35 DAP). Nomenclature: Clomazone, glyphosate, imazethapyr, horseweed, Conyza (= Erigeron) canadensis L. #3 ERICA; fall panicum, Panicum dichotomiflorum Michx. # PANDI. Additional index words: Preplant glyphosate applications, critical weed period, integrated weed management, Amaranthus hybridus, Ambrosia artemisiifolia, Chenopodium album, Ipomea hederacea, Xanthium strumarium, AMACH, AMBEL, CHEAL, IPOHE, SETFA, XANST. Abbreviations: DAP, days after planting; GRS, glyphosate-resistant soybean; POST, postemergence; RAREC, Rutgers Agricultural Research and Extension Center; UD-REC, University of Delaware Research and Education Center.

Optimum Glyphosate Timing with or without Residual Herbicides in Glyphosate-Resistant Soybean (Glycine max) under Full-Season Conventional Tillage1

Abstract: Field studies were conducted under full-season conventional tillage in Delaware and New Jersey to determine the critical time to apply glyphosate with or without residual herbicides for optimum weed control in glyphosate-resistant soybean (GRS). The residual herbicides tank-mixed with glyphosate (0.84 kg/ha) were clomazone (0.55 kg/ha) and imazethapyr (0.063 kg/ha). Herbicide application was made at cracking, unifoliate, and one- to six-trifoliate stages of GRS. Weeds varied in growth stages from preemergence (PRE) at cracking to an average height of 30 cm at the six-trifoliate stage of GRS. Herbicide activity varied by year and weed species. Herbicidal action was better under high (>125 mm/mo) than low (<100 mm/mo) rainfall regime. Glyphosate application without residual herbicides was less effective at cracking and unifoliate than at one- to three-trifoliate leaf stages. Mixing residual herbicides with glyphosate at cracking and unifoliate stages enhanced weed control but made no difference when application was delayed until one- to three-trifoliate stages. For optimum weed control in GRS, the window of application for glyphosate alone was between the one- and three-trifoliate leaf stages, approximately 18 to 28 days after planting (DAP). If glyphosate was tank-mixed with residual herbicides, the window of application extended from cracking until the four-trifoliate stage; and weed interference until the four-trifoliate stage (approximately 32 DAP) did not depress GRS yield. Nomenclature: Clomazone, 2-[(2-chlorophenyl)methyl]-4,4-dimethyl-3-isoxazolidinone; glyphosate, N-(phosphonomethyl)glycine; imazethapyr, 2-[4,5-dihydro-4-methyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl]-5-ethyl-3-pyridinecarboxylic acid. Additional index words: Critical weed removal period, glyphosate ± residuals, Amaranthus hybridus, Ambrosia artemisiifolia, Chenopodium album, Ipomoea hederacea, Panicum dichotomiflorum, AMACH, AMBEL, CHEAL, IPOHE, PANDI. Abbreviations: DAP, days after planting; GRS, glyphosate-resistant soybean; POST, postemergence; PRE, preemergence; RAREC, Rutgers Agricultural Research and Extension Center; UD-REC, University of Delaware Research and Education Center.

Effect of postemergence glyphosate application timing on weed control and grain yield in glyphosate-resistant corn: Results of a 2-yr multistate study

Field studies were conducted at 35 sites throughout the north-central United States in 1998 and 1999 to determine the effect of postemergence glyphosate application timing on weed control and grain yield in glyphosate-resistant corn. Glyphosate was applied at various timings based on the height of the most dominant weed species. Weed control and corn grain yields were considerably more variable when glyphosate was applied only once. The most effective and consistent season-long annual grass and broadleaf weed control occurred when a single glyphosate application was delayed until weeds were 15 cm or taller. Two glyphosate applications provided more consistent weed control when weeds were 10 cm tall or less and higher corn grain yields when weeds were 5 cm tall or less, compared with a single application. Weed control averaged at least 94 and 97% across all sites in 1998 and 1999, respectively, with two glyphosate applications but was occasionally less than 70% because of late emergence of annual grass and Amaranthus spp. or reduced control of Ipomoea spp. With a single application of glyphosate, corn grain yield was most often reduced when the application was delayed until weeds were 23 cm or taller. Averaged across all sites in 1998 and 1999, corn grain yields from a single glyphosate application at the 5-, 10-, 15-, 23-, and 30-cm timings were 93, 94, 93, 91, and 79% of the weed-free control, respectively. There was a significant effect of herbicide treatment on corn grain yield in 23 of the 35 sites when weed reinfestation was prevented with a second glyphosate application. When weed reinfestation was prevented, corn grain yield at the 5-, 10-, and 15-cm application timings was 101, 97, and 93% of the weed-free control, respectively, averaged across all sites. Results of this study suggested that the optimum timing for initial glyphosate application to avoid corn grain yield loss was when weeds were less than 10 cm in height, no more than 23 d after corn planting, and when corn growth was not more advanced than the V4 stage. Nomenclature: Glyphosate; Amaranthus spp. #3 AMASS; Ipomoea spp. # IPOSS; corn, Zea mays L. ‘Roundup Ready®’ # SETFA. Additional index words: Herbicide-resistant crops, weed interference. Abbreviation: POST, postemergence.

Herbicides for Potential Use in Lima Bean (Phaseolus lunatus) Production

Abstract: Herbicides registered for lima bean (Phaseolus lunatus L.) do not consistently control many troublesome weeds. Some herbicides registered for soybean (Glycine max) will control these weeds, but tolerance to lima bean is not known. Two field and two greenhouse studies were conducted to evaluate recently registered soybean herbicides for lima bean tolerance. Field studies were conducted in Delaware from 1996 to 1998, and in North Carolina during 1997 and 1998. The first field study evaluated the preemergence (PRE) herbicides cloransulam at 0.01, 0.02, 0.03, and 0.04 kg ai/ha; flumetsulam at 0.04, 0.05, 0.06, and 0.07 plus metolachlor at 1.3, 1.6, 1.8, and 2.1 kg ai/ha; sulfentrazone at 0.1, 0.15, 0.2, and 0.25 kg ai/ha; lactofen at 0.2 and 0.25 kg ai/ha; and the commercial standard treatment of imazethapyr plus metolachlor at 0.05 and 1.7 kg ai/ha, respectively. Lima bean injury 5 to 8 wk after emergence was lowest for imazethapyr plus metolachlor (standard treatment) and all four rates of cloransulam. Crop injury with flumetsulam plus metolachlor ranged from 0 to 18% and sulfentrazone ranged from 3 to 75% depending on location and rate. Lactofen treatments caused unacceptable lima bean injury. Yield in plots treated with cloransulam were consistently greater than in the plots treated with other herbicides. The second field study examined the postemergence (POST) herbicides cloransulam (0.013 or 0.02 kg ai/ha), bentazon (1.1 kg ai/ha), imazethapyr (0.035 or 0.053 kg ai/ha), and imazamox (0.018 or 0.036 kg ai/ha), applied when the crop was at the first trifoliolate stage. Cloransulam caused 0 to 13% crop injury and imazamox caused 3 to 25% injury depending on rate and location. In greenhouse studies, no differences were observed among eight common processing lima bean cultivars in tolerance to sulfentrazone applied PRE or to cloransulam, imazamox, imazethapyr, or bentazon applied POST. Nomenclature: Bentazon, 3-(1-methylethyl)-(1H)-2,1,3-benzothiadiazin-4(3H)-one 2,2-dioxide; cloransulam, 3-chloro-2-[[(5-ethoxy-7-fluoro[1,2,4]triazolo[1,5-c]pyrimidine-2yl)sulfonyl]amino]benzoic acid; flumetsulam, N-(2,6-difluorophenyl)-5-methyl[1,2,4]triazolo[1,5-α]pyrimidine-2-sulfonamide; imazamox, 2-[4,5-dihydro-4-methyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl]-5-(methoxymethyl)-3-pyridinecarboxylic acid; imazethapyr, 2-[4,5-dihydro-4-methyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl]-5-ethyl-3-pyridinecarboxylic acid; lactofen, (±)-2-ethoxy-1-methyl-2-oxoethyl-5-[2-chloro-4-(trifluoromethyl)phenoxy]-2-nitrobenzoate; metolachlor, 2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-1-methylethyl)acetamide; sulfentrazone, N-[2,4-dichloro-5-[4-(difluoromethyl)-4,5-dihydro-3-methyl-5-oxo-1H-1,2,4-triazol-1-yl]phenyl]methanesulfonamide; lima bean, Phaseolus lunatus L., ‘M-15’, ‘F1072’, ‘M-408’, ‘Packers’, ‘Concentrated Fordhook’, ‘8-78’, ‘Eastland’; soybean, Glycine max (L.) Merr. Additional index words: Crop tolerance; varietal sensitivity. Abbreviations: COC, crop oil concentrate; NIS, nonionic surfactant; POST, postemergence; PRE, preemergence; WAT, weeks after treatment.

Transfer of glyphosate resistance: evidence of hybridization in Conyza (Asteraceae)

Transfer of herbicide resistance genes between crops and weeds is relatively well documented; however, far less information exists for weed-to-weed interactions. The hybridization between the weedy diploids Conyza canadensis (2n = 18) and C. ramosissima (2n = 18) was investigated by monitoring transmission of the allele conferring resistance to N-phosphonomethyl glycine (glyphosate). In a multivariate quantitative trait analysis, we described the phylogenic relationship of the plants, whereas we tested seed viability to assess potential postzygotic reproductive barriers (PZRB) thus affecting the potential establishment of hybrid populations in the wild. When inflorescences were allowed to interact freely, approximately 3% of C. ramosissima or C. canadensis ova were fertilized by pollen of the opposing species and produced viable seeds; >95% of the ova were fertilized under no-pollen competition conditions (emasculation). The interspecific Conyza hybrid ( ) demonstrated an intermediate phenotype between the parents but superior resistance to glyphosate compared to the resistant C. canadensis parent. Inheritance of glyphosate resistance in the selfed ( ) followed the partially dominant nuclear, single-gene model; backcrosses confirmed successful introgression of the resistance allele to either parent. Negligible PZRB were observed in the hybrid progenies, confirming fertility of the C. canadensis × C. ramosissima nothotaxa. The implications of introgressive hybridization for herbicide resistance management and taxonomy of Conyza are discussed.

Influence of Glyphosate-Resistant Horseweed (Conyza Canadensis) Growth Stage on Response to Glyphosate Applications

Abstract Infestations of glyphosate-resistant (GR) horseweed have become widespread in the eastern United States. This biotype is problematic in no-tillage production that relies extensively on glyphosate for weed control. Because horseweed is treated at various stages of growth, a greenhouse study explored rate response of glyphosate-resistant and -susceptible horseweed at three growth stages. GR horseweed was more responsive to glyphosate at the seedling stage than at the large rosette or bolting stages. A field study evaluated GR horseweed response when treated with glyphosate at soybean planting time, POST in-crop (about 45 d after planting), or both at planting and POST in-crop. There was a cumulative effect of the at-planting followed by POST in-crop glyphosate applications. When evaluating single glyphosate applications, the at-planting application was more effective at suppressing GR horseweed than a POST in-crop application. Because glyphosate cannot control GR horseweed, this biotype should be controlled with an herbicide with an alternate mode of action and applied at the most effective timing. Nomenclature: Glyphosate; horseweed, Conyza canadensis (L.) Cronq.; soybean, Glycine max (L.) Merr

The Effect of Weed Density and Application Timing on Weed Control and Corn Grain Yield1

A 2-yr experiment repeated at five locations across the northeastern United States evaluated the effect of weed density and time of glyphosate application on weed control and corn grain yield using a single postemergence (POST) application. Three weed densities, designed to reduce corn yields by 10, 25, and 50%, were established across the locations, using forage sorghum as a surrogate weed. At each weed density, a single application of glyphosate at 1.12 kg ai/ha was applied to glyphosate-resistant corn at the V2, V4, V6, and V8 growth stages. At low and medium weed densities, the V4 through V8 applications provided nearly complete weed control and yields equivalent to the weed-free treatment. Weed biomass and the potential for weed seed production from subsequent weed emergence made the V2 timing less effective. At high weed densities, the V4 followed by the V6 timing provided the most effective weed control, while maintaining corn yield. Weed competition from subsequent weed emergence in the V2 application and the duration of weed competition in the V8 timing reduced yield on average by 12 and 15%, respectively. This research shows that single POST applications can be successful but weed density and herbicide timing are key elements. Nomenclature: Glyphosate; corn, Zea mays L.; forage sorghum, Sorghum bicolor (L.) Moench. Additional index words: Glyphosate-resistant corn, herbicide application timing, postemergence weed control, weed competition, weed density. Abbreviations: POST, postemergence; WAP, weeks after planting.