Márta Erdélyi

Effect of combined treatment with aflatoxin B1 and T-2 toxin and metabolites on some production traits and lipid peroxide status parameters of broiler chickens.

Three groups of cockerels were fed with a control diet, with a diet contaminated with T-2 and HT-2 toxin (0.31 and 0.26 mg/kg) or with that containing a combination of T-2 and HT-2 toxin (0.32 and 0.25 mg/kg) and aflatoxin B1 (AFB1, 0.38 mg/kg) for 21 days. Body weight gain and feed conversion ratio did not differ significantly among the groups. Malondialdehyde concentration of the liver was lower in the group fed the diet contaminated with the combination of T-2 + HT-2 toxin and aflatoxin B1 as compared to the control group or the group fed T-2 + HT-2 toxins. Reduced glutathione (GSH) content of the liver was lower in the T-2 + HT-2 group than in the group fed a combination of T-2, HT-2 and aflatoxin. Reduced glutathione content of the heart was higher in the T-2 + HT-2 group than in the control group. Mycotoxin contamination had no effect on glutathione peroxidase (GSH-Px) activity in comparison to the control, but significantly lower GSH-Px activity was found in the heart of chickens in the T-2 + HT-2 + AFB1 group than in the T-2 + HT-2 group. In this study, T-2 + HT-2 toxin and aflatoxin B1 contamination of the diets did not affect the production traits adversely and did not exert additive effects on lipid peroxidation and on the glutathione redox system.

Effects of ochratoxin a on some production traits, lipid peroxide and glutathione redox status of weaned piglets

The effect of feeding ochratoxin A (OTA) contaminated diet (379.6 and 338.1 microg/kg in starter and grower diets) on production traits, lipid peroxidation and some parameters of the glutathione redox system were investigated in weaned piglets over a seven-week period. Feed intake and feed conversion ratio (FCR) did not differ significantly, but in the first phase (0-28 days) the daily weight gain was significantly lower in the piglets fed the OTA-contaminated diet. Lipid peroxidation, as measured by the amount of malondialdehyde, glutathione content and glutathione peroxidase activity, did not change significantly in the blood plasma and red blood cell haemolysate in the OTA-loaded group, while malondialdehyde content increased significantly in the liver and markedly but not significantly in the kidney of piglets fed OTA-contaminated feed. Glutathione content did not differ significantly in the studied organs of the two groups while glutathione peroxidase activity of the OTA-loaded animals was significantly lower both in the liver and in the kidney. The results suggest that the use of feed-stuffs contaminated with low levels of OTA for seven weeks did not cause marked differences in the production traits or in lipid peroxidation and amount or activity of the glutathione redox system in the blood plasma, red blood cells and kidney, while significant changes occurred in the liver homogenate.

Effect of diets with different inclusion levels of distillers dried grain with solubles combined with lysine and methionine supplementation on the lipid peroxidation and glutathione status of chickens

To study the possible effects of different inclusion levels of distillers dried grain with solubles (DDGS) on the lipid peroxidation and glutathione redox status of chickens, 200 three-week-old Ross 308 cockerels were assigned to four treatment groups of 50 birds each. The groups were fed a control and three experimental, isocaloric and isonitrogenous grower diets containing 15, 20 and 25% DDGS, respectively, combined with lysine (Lys) and methionine (Met) supplementation until 6 weeks of age. It was found that DDGS inclusion increased the ether extract content of the diets which resulted in higher reduced glutathione (GSH) content and elevated glutathione peroxidase activity (GSHPx) in the liver. However, DDGS addition with Lys and Met supplementation did not influence the malondialdehyde content of the blood and the liver. The oleic acid proportion of the diet showed a close positive correlation with GSH content of the liver. A smaller ratio of methionine and cysteine in the diet with DDGS resulted in significantly higher liver GSH content. GSHPx activity increased parallel with the elevated GSH content of the liver homogenate, suggesting that the enzyme is activated by the actual supply of its co-substrate. In conclusion, the results show that DDGS, even at a high inclusion level combined with Lys and Met supplementation, has no initiative effect on lipid peroxidation in the blood and liver of broiler chickens.

Short-term effects of T-2 toxin or deoxynivalenol on glutathione status and expression of its regulatory genes in chicken

Short-term (48-hour) effects of 3.74/1.26 mg kg-1 T-2/HT-2 toxin or 16.12 mg kg-1 DON in feed were investigated in the liver of three-week-old cockerels (body weight: 749.60 ± 90.98 g). Markers of lipid peroxidation showed no significant changes. At hour 24, glutathione content in the T-2/HT-2 toxin group was significantly higher than in the control. Glutathione peroxidase activity was significantly higher than the control at hour 24 in the T-2/H-2 toxin group and at hour 48 in the DON group. In the DON group, expression of the glutathione peroxidase 4 gene (GPX4) was significantly lower than in the control at hours 12 and 14, and higher at hour 48. Expression of the glutathione reductase gene (GSR) was significantly lower than in the control at hour 12 in the T-2/HT-2 toxin group, and at hours 12, 24 and 48 in the DON group. However, at hour 36 higher GSR expression was measured in the DON group. Due to the effect of both trichothecenes, expression of the glutathione synthetase gene (GSS) was significantly lower than in the control at hours 24 and 48. In conclusion, T-2/HT-2 toxin and DON had a moderate short-term effect on free radical formation. T-2/HT-2 toxin induced more pronounced activation of the glutathione redox system than did DON.

Multi-trichothecene mycotoxin exposure activates glutathione-redox system in broiler chicken

ABSTRACT Co‐occurrence of mycotoxin contamination of feeds is a frequent problem, therefore the purpose of this study was to evaluate the combined effect of T‐2 toxin and deoxynivalenol (DON) on lipid peroxidation, parameters and regulation of the glutathione redox system in broiler chickens in a sub‐chronic (7 day) study. The applied doses were: low mix: 0.23mg T‐2 toxin and 4.96mg DON/kg feed; medium mix: 1.21mg T‐2 toxin and 12.38mg DON/kg feed; and high mix: 2.42 T‐2 toxin and 24.86mg DON/kg feed. Liver samples were taken on days 0, 1, 2, 3, and 7 of the feeding trial. Lipid peroxidation decreased significantly as compared to the control on days 3 and 7 as effect of low and high doses, which can be related to the activation of the antioxidant system, which is supported by the elevated glutathione peroxidase activity and reduced glutathione concentration as compared to the control on day 3 in the medium and high dose groups. Gene expression of glutathione peroxidase 4 (GPX4) elevated on day 1 in a dose dependent manner, and showed continuous elevation in the highest dose group thereafter. The results suggested that common exposure of T‐2 toxin and DON induced oxidative stress in the liver of broiler chickens, which activated the enzymatic antioxidant system, and consequently decreased lipid peroxidation. HIGHLIGHTSCommon short‐term exposure to T‐2 toxin and DON decreased their known individual effect on feed refusal.Also, it was induced mild oxidative stress in the liver of chicken and increased expression of glutathione peroxidase 4 gene.Glutathione redox system responded rapidly and firmly.

Role of Glutathione Redox System on the T-2 Toxin Tolerance of Pheasant ( Phasianus colchicus )

The purpose of the present study was to evaluate the effects of different dietary concentrations of T-2 toxin on blood plasma protein content, lipid peroxidation and glutathione redox system of pheasant (Phasianus colchicus). A total of 320 one-day-old female pheasants were randomly assigned to four treatment groups fed with a diet contaminated with different concentrations of T-2 toxin (control, 4 mg/kg, 8 mg/kg and 16 mg/kg). Birds were sacrificed at early (12, 24 and 72 hr) and late (1, 2 and 3 weeks) stages of the experiment to demonstrate the effect of T-2 toxin on lipid peroxidation and glutathione redox status in different tissues. Feed refusal and impaired growth were observed with dose dependent manner. Lipid-peroxidation was not induced in the liver, while the glutathione redox system was activated partly in the liver, but primarily in the blood plasma. Glutathione peroxidase activity has changed parallel with reduced glutathione concentration in all tissues. Based on our results, pheasants seem to have higher tolerance to T-2 toxin than other avian species, and glutathione redox system might contribute in some extent to this higher tolerance, in particular against free-radical mediated oxidative damage of tissues, such as liver.

Az élelmiszerek rosttartalmának antioxidáns hatása

Absztrakt: Az antioxidansok az egeszsegre szamos kedvező hatassal rendelkeznek, amelyek kozul napjainkig keves figyelem iranyult az elelmi rost antioxidans tulajdonsagaira. Az elelmi rost antioxidans hatasa mogott az azt alkoto poliszacharidkomplexhez kapcsolt polifenolvegyuletek allnak, amelyek a belcsatornaban felszabadulva fejtik ki hatasukat. Novenyi sejtfalbol izolaltak egy, az előbbiektől elterő molekulat, a kalcium-fruktoboratot, amelynek szinten van antioxidans kapacitasa, annak aktiv komponense azonban a bor. Szamos olyan elelmiszer es elelmiszeripari mellektermek ismert, amelynek jelentős antioxidans elelmirost-tartalma van, igy ezek is hozzajarulhatnak a belcsatorna, ennek reven a szervezet antioxidans vedelmehez. Ilyenek peldaul a gabonamagvak, a kaposzta, a kavebab es a guava, vagy a mellektermekek kozul a szőlőtorkoly. Az antioxidans elelmi rost polifenolvegyuletei az egyes novenyekben elterőek, igy azok antioxidans kapacitasa is valtozo, de grammonkent hozzavetőlegesen 50–100 mg DL-α-tokofe...

Some chemical and physical characteristics of farmed pheasant hens (Phasianus cholchicus) breast meat

Ring necked pheasant is the most significant game bird in Hungary. Around 300.000 pheasant harvested (hunted) annually and generally these birds are consumed by the hunters. As there are limited data on the quality of pheasant meat, in the present study we aimed to analyze some physical and chemical properties of it. At 20 weeks of age 63 pheasant hens were exterminated by cervical dislocation and meat, liver, spleen and heart samples were taken. The live weight of the birds was 1045±92g (870 to 1300g). The average weight of the liver, spleen and heart was 14.12±2.58, 0.47±0.13 and 4.30±0.49, respectively. The average drip loss was 5.90±2.38% (0.68±0.28g). As was expected the average protein content (26.2±0.7%) of the pheasant breasts was markedly higher than in broiler or turkey. The average fat content (0.4±0.2%) was similar to that in turkey. The unique chemical and physical properties of the pheasant meat make it suitable to fit in the human nutrition.

EFFECT OF SHORT-TERM AFLATOXIN EXPOSURE IN COMBINATION WITH MEDICINAL HERB MIXTURE ON LIPID PEROXIDATION AND GLUTATHIONE REDOX SYSTEM IN LAYING HENS

Aflatoxins are well known hepatotoxic mycotoxins, which mainly contaminate the cereal grains. Those induce lipid peroxidation and impair the antioxidant, including glutathione redox system in long-term studies. The purpose of present study was to investigate the short-term (36-hour) effect of feeding aflatoxin B1 (AFB1) contaminated diet alone or in combination with a medicinal herb mixture on lipid peroxidation (conjugated dienes and trienes, and malondialdehyde), and on parameters of the glutathione system (reduced glutathione and glutathione peroxidase) in blood plasma, red blood cell haemolysate, liver and kidney homogenate of 49-week old Bovans Goldline laying hens. The results revealed that AFB1 (125 m /kg feed) did not have effect on feed intake, body and liver weight, but increased malondialdehyde content was observed in blood plasma and red blood cell haemolysate as effect of feeding AFB1 and medicinal herb mixture at 36th hour of the trial. However, the same diet resulted in lower malondialdehyde content in liver, but not in kidney. Reduced glutathione concentrations showed variance among treatments; thus due to inclusion of medicinal herb mixture in the diet lower values were measured in red blood cell haemolysate. Glutathione peroxidase activity was significantly lower in all treated groups as compared to the control at 36th hour of the trial in blood plasma, but not in other tissues. The results are contradictory with previous findings, probably due to the short-term exposure, and/or to medicinal herb mixture supplementation as it could moderately modify the effect of AFB1.