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Featured researches published by Martine Bastien.


Emerging Infectious Diseases | 2008

Human bocavirus infections in hospitalized children and adults.

Jean Longtin; Martine Bastien; Rodica Gilca; Éric Leblanc; Gaston De Serres; Michel G. Bergeron; Guy Boivin

The pathogenic role of this virus in infected children is unclear.


Journal of Clinical Microbiology | 2009

Internal Control for Nucleic Acid Testing Based on the Use of Purified Bacillus atrophaeus subsp. globigii Spores

François J. Picard; Martin Gagnon; Marthe R. Bernier; Nicholas J. Parham; Martine Bastien; Maurice Boissinot; Régis Peytavi; Michel G. Bergeron

Commonly used internal controls (ICs) to monitor the efficiency of nucleic acid testing (NAT) assays do not allow verification of nucleic acid extraction efficiency. Since microbial cells are often difficult to lyse, it is important to ensure that nucleic acids are efficiently extracted from any target organism. For this purpose, we developed a cellular IC based on the use of nonpathogenic Bacillus spores. Purified Bacillus atrophaeus subsp. globigii (referred to hereafter as simply B. atrophaeus) spores were added to vaginal and anal samples, which were then subjected to rapid DNA extraction and subsequent PCR amplification. The proof of concept of this cellular IC was made through the use of both manual and automated DNA extraction methods, using vaginal or anal samples spiked with B. atrophaeus spores, combined with a multiplex real-time PCR assay for the specific detection of group B streptococci (GBS) and B. atrophaeus. The performance of the cellular IC was compared to that of a standard IC plasmid added to PCRs. Approximately 500 B. atrophaeus spores per PCR was found to be optimal since this did not interfere significantly with GBS detection for either DNA extraction method and yielded reproducible amplification and/or detection of B. atrophaeus genomic DNA serving as an IC template. Performance of the cellular IC was comparable to that of the standard IC. This novel IC system using nonpathogenic and hard-to-lyse B. atrophaeus spores allowed validation of both the DNA extraction procedure and the amplification and detection process. Use of a spore-based control also provides a universal control for microbial cell lysis.


Applied and Environmental Microbiology | 2012

Rapid Filtration Separation-Based Sample Preparation Method for Bacillus Spores in Powdery and Environmental Matrices

Sandra Isabel; Maurice Boissinot; Isabelle Charlebois; Chantal M. Fauvel; Lu-E Shi; Julie-Christine Lévesque; Amélie T. Paquin; Martine Bastien; Gale Stewart; Éric Leblanc; Sachiko Sato; Michel G. Bergeron

ABSTRACT Authorities frequently need to analyze suspicious powders and other samples for biothreat agents in order to assess environmental safety. Numerous nucleic acid detection technologies have been developed to detect and identify biowarfare agents in a timely fashion. The extraction of microbial nucleic acids from a wide variety of powdery and environmental samples to obtain a quality level adequate for these technologies still remains a technical challenge. We aimed to develop a rapid and versatile method of separating bacteria from these samples and then extracting their microbial DNA. Bacillus atrophaeus subsp. globigii was used as a simulant of Bacillus anthracis. We studied the effects of a broad variety of powdery and environmental samples on PCR detection and the steps required to alleviate their interference. With a benchmark DNA extraction procedure, 17 of the 23 samples investigated interfered with bacterial lysis and/or PCR-based detection. Therefore, we developed the dual-filter method for applied recovery of microbial particles from environmental and powdery samples (DARE). The DARE procedure allows the separation of bacteria from contaminating matrices that interfere with PCR detection. This procedure required only 2 min, while the DNA extraction process lasted 7 min, for a total of <10 min. This sample preparation procedure allowed the recovery of cleaned bacterial spores and relieved detection interference caused by a wide variety of samples. Our procedure was easily completed in a laboratory facility and is amenable to field application and automation.


Lab on a Chip | 2010

Validation of a centrifugal microfluidic sample lysis and homogenization platform for nucleic acid extraction with clinical samples

Jonathan Siegrist; Robert Gorkin; Martine Bastien; Gale Stewart; Régis Peytavi; Horacio Kido; Michel G. Bergeron; Marc Madou


Archive | 2003

Biological reagents and methods to verify the efficiency of sample preparation and nucleic acid amplification and/or detection

Jean Pierre Gayral; François J. Picard; Maurice Boissinot; Martine Bastien


Archive | 2003

Method for the preparation of reagents for amplification and/or detection of nucleic acids that exhibit no significant contamination by nucleic acids

François J. Picard; Christian Ménard; Martine Bastien; Maurice Boissinot


Microfluidics, BioMEMS, and Medical Microsystems IV | 2006

Rapid and automated sample preparation for nucleic acid extraction on a microfluidic CD (compact disk)

Jitae Kim; Horacio Kido; Jim Zoval; Dominic Gagné; Régis Peytavi; François J. Picard; Martine Bastien; Maurice Boissinot; Michel G. Bergeron; Marc Madou


Archive | 2003

Methods to verify the efficiency of sample preparation and nucleic acid amplification and/or detection

Jean-Pierre Gayral; François J. Picard; Maurice Boissinot; Martine Bastien


Archive | 2003

Reactifs biologiques et procedes permettant de verifier l'efficacite de la preparation d'un echantillon et de l'amplification et/ou de la detection d'un acide nucleique

Martine Bastien; Maurice Boissinot; Jean-Pierre Gayral; François J. Picard


Archive | 2003

Methoden zur überprüfung der effizienz der probenaufbereitung und der vervielfältigung und/oder des nachweises von nukleinsäuren.

Jean-Pierre Gayral; François J. Picard; Maurice Boissinot; Martine Bastien

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Horacio Kido

University of California

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Marc Madou

University of California

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