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Dive into the research topics where Masafumi Shimada is active.

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Featured researches published by Masafumi Shimada.


Applied and Environmental Microbiology | 2001

Distribution and Diversity of Symbiotic Thermophiles, Symbiobacterium thermophilum and Related Bacteria, in Natural Environments

Kenji Ueda; Michiyo Ohno; Kaori Yamamoto; Hanae Nara; Yujiro Mori; Masafumi Shimada; Masahiko Hayashi; Hanako Oida; Yuko Terashima; Mitsuyo Nagata; Teruhiko Beppu

ABSTRACT Symbiobacterium thermophilum is a tryptophanase-positive thermophile which shows normal growth only in coculture with its supporting bacteria. Analysis of the 16S rRNA gene (rDNA) indicated that the bacterium belongs to a novel phylogenetic branch at the outermost position of the gram-positive bacterial group without clustering to any other known genus. Here we describe the distribution and diversity of S. thermophilum and related bacteria in the environment. Thermostable tryptophanase activity and amplification of the specific 16S rDNA fragment were effectively employed to detect the presence of Symbiobacterium. Enrichment with kanamycin raised detection sensitivity. Mixed cultures of thermophiles containing Symbiobacterium species were frequently obtained from compost, soil, animal feces, and contents in the intestinal tracts, as well as feeds. Phylogenetic analysis and denaturing gradient gel electrophoresis of the specific 16S rDNA amplicons revealed a diversity of this group of bacteria in the environment.


Fisheries Science | 2009

Phylogeography of the Japanese eight-barbel loach Lefua echigonia from the Yamagata area of the Tohoku district, Japan

Hiroshi Aiki; Katsuhiko Takayama; Takashi Tamaru; Nobuhiro Mano; Masafumi Shimada; Hiroshi Komaki; Hitomi Hirose

The Japanese eight-barbel loach Lefua echigonia, which is a freshwater fish native to Japan, is distributed from the Tohoku to Kinki districts and is divided into six regional populations according to mtDNA analysis. In this study, we investigated L. echigonia collected from several locations in Yamagata Prefecture and neighboring prefectures using mtDNA control region sequences and confirmed the spatial distribution pattern among the new regional population (Yamagata population). The new population was limited to the Mogami river system in the inland area of Yamagata Prefecture and is distinguished from other regional populations by high sequence divergences.


Journal of the Japanese Institute of Landscape Architecture | 1989

The Current Status and Tasks for the Future of Environmental Education in the Tama River

Yorikazu Maruta; Masafumi Shimada; Osamu Murakami; Hisako Ito

都市環境の保全や改良にあたっては, 様々な場面に応じた環境教育の必要性が論じられている。本報においては, 大都市圏に位置し, 上流から下流にかけて各種の環境を有する多摩川を事例として取り上げ, また, 環境教育の実態については, その体系上, 最も基礎的な部分を担う小・中学校の環境教育を対象にアンケート調査を行い, 活動の内容や実施上の問題点等について把握するとともに今後の環境教育の課題等について考察を加えた。


Journal of the Japanese Institute of Landscape Architecture | 1988

Fundamental Study on the Planning of Nature Conservation for Inhabitant Small Animals and Insects in Tokyo Ward

Yorikazu Maruta; Masafumi Shimada; Shigeto Yanai

自然の小動物が生息しうる潤いのある都市環境の形成を図っていくことは, 重要な課題と言える。本研究は, 東京都杉並区を研究対象地とし, 緑の分布特性や住民意識調査を通じて, 小動物の生息からみた緑の保全等の方向性を探ってみた。その結果, 緑被地の状況と小動物の生息状況については4タイプに分類され, 生息にあたっての核的存在となる公園緑地の確保やそのネットワーク化, さらには, 地域ぐるみの緑化の必要性等が把握された。


Journal of the Japanese Institute of Landscape Architecture | 1983

Studies on Landscape Planning by Greens

Yorikazu Maruta; Masafumi Shimada; Kunio Abe

都市景観を構成する緑の配置のあり方に関して, 視覚的アプローチから検討したものである。特に, 市街地の中景の対象物となる高木等の視覚的影響範囲を把握するために, 公園等4箇所のモデル地区を選び踏査した結果概ね200~250mが, これら緑の視覚的影響範囲としてとらえられた。さらに, この結果に加え, 以前行われた諸文献との対応性を図り, 今後の都市景観計画との関連性において, 緑の配置方法を論及したものてある。


Nucleic Acids Research | 2004

Genome sequence of Symbiobacterium thermophilum, an uncultivable bacterium that depends on microbial commensalism

Kenji Ueda; Atsushi Yamashita; Jun Ishikawa; Masafumi Shimada; Tomo-o Watsuji; Kohji Morimura; Haruo Ikeda; Masahira Hattori; Teruhiko Beppu


Journal of the Japanese Institute of Landscape Architecture | 1990

Studies on lnhabitant Environment of lnsects in Parks and Open Spaces in Urban Area

Masafumi Shimada; Tetsuo Takahashi; Yorikazu Maruta


Journal of the Japanese Institute of Landscape Architecture | 1987

Studies on Inhabitant Insects in Parks and Open Spaces, covered with Substitution Forest, in Suburban Areas

Masafumi Shimada; Yorikazu Maruta


Aquaculture Science | 1999

Parasites of Aquatic Animals and Water Quality in an Urban Reservoir

Hitomi Hirose; Nobuhiro Mano; Masafumi Shimada


Journal of the Japanese Institute of Landscape Architecture | 1986

Fundamental Study of Avifauna and Land-use in the Urban Area

Namiko Shibuya; Masafumi Shimada; Yorikazu Maruta

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