Masakazu Shimizu
Hiroshima University
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Publication
Featured researches published by Masakazu Shimizu.
Journal of Bone and Mineral Research | 2004
Takehiro Matsubara; Ketut Suardita; Masakazu Ishii; Masaru Sugiyama; Akira Igarashi; Ryo Oda; Masahiro Nishimura; Masahiro Saito; Keigo Nakagawa; Katsuyuki Yamanaka; Kazuko Miyazaki; Masakazu Shimizu; Ujjal K. Bhawal; Koichiro Tsuji; Kozo Nakamura; Yukio Kato
We isolated and expanded BMSCs from human alveolar/jaw bone at a high success rate (70%). These cells had potent osteogenic potential in vitro and in vivo, although their chondrogenic and adipogenic potential was less than that of iliac cells.
Genes to Cells | 2009
Hiroshi Kubo; Masakazu Shimizu; Yuji Taya; Takeshi Kawamoto; Masahiko Michida; Emi Kaneko; Akira Igarashi; Masahiro Nishimura; Kazumi Segoshi; Yoshihito Shimazu; Koichiro Tsuji; Takaaki Aoba; Yukio Kato
Although ex vivo expanded mesenchymal stem cells (MSC) have been used in numerous studies, the molecular signature and in vivo distribution status of MSC remain unknown. To address this matter, we identified numerous human MSC‐characteristic genes—including nine transcription factor genes —using DNA microarray and real‐time RT‐PCR analyses: Most of the MSC‐characteristic genes were down‐regulated 24 h after incubation with osteogenesis‐, chondrogenesis‐ or adipogenesis‐induction medium, or 48–72 h after knockdown of the nine transcription factors. Furthermore, knockdowns of ETV1, ETV5, FOXP1, GATA6, HMGA2, SIM2 or SOX11 suppressed the self‐renewal capacity of MSC, whereas those of FOXP1, SOX11, ETV1, SIM2 or PRDM16 reduced the osteogenic‐ and/or adipogenic potential. In addition, immunohistochemistry using antibodies for the MSC characteristic molecules—including GATA6, TRPC4, FLG and TGM2—revealed that MSC‐like cells were present near the endosteum and in the interior of bone marrow of adult mice. These findings indicate that MSC synthesize a set of MSC markers in vitro and in vivo, and that MSC‐characteristic transcription factors are involved in MSC stemness regulation.
Archive | 2006
Yukio Kato; Takeshi Kawamoto; Koichiro Tsuji; Akira Igarashi; Masakazu Shimizu
Analytical Sciences | 2003
Masakazu Shimizu; Noriyuki Ojima; Hitoshi Ohnishi; Tomoteru Shingaki; Yasuyuki Hirakawa; Tsutomu Masujima
Analytical Sciences | 2002
Masakazu Shimizu; Francesca Levi-Schaffer; Noriyuki Ojima; Tomoteru Shingaki; Tsutomu Masujima
Rapid Communications in Mass Spectrometry | 2007
Anusak Sirikatitham; Takahiro Yamamoto; Masakazu Shimizu; Tomomi Hasegawa; Naohiro Tsuyama; Tsutomu Masujima
Archive | 2004
Tetsuaki Hara; Yukio Kato; Masakazu Shimizu; Kinshiyo Sho; 幸夫 加藤; 鐵晃 原; 正和 清水; 金昌 邵
Bunseki Kagaku | 2004
Yasuyuki Hirakawa; Masakazu Shimizu; Tsutomu Masujima
Archive | 2006
Yukio Kato; Takeshi Kawamoto; Koichiro Tsuji; Akira Igarashi; Masakazu Shimizu
Archive | 2006
Akira Igarashi; Yukio Kato; Takeshi Kawamoto; Masakazu Shimizu; Koichiro Tsuji