Masanobu Fujii

A comparative study of the ability of ferric nitriloacetate and other iron chelators to assist membrane lipid peroxidation by superoxide radicals

Abstract This study examined some of the variables determining the efficiency of lipid peroxidation in egg yolk phosphatidylcholine liposomes and in microsomes exposed to enzymatically-generated superoxide radicals. The initiation of peroxidation required the presence of preformed lipid peroxides and a chelated metal catalyst. Comparison of the relative effectiveness of four iron chelating agents showed that the chelate must bind to the membrane by coulombic attraction between the charged membrane and a chelate carrying an opposite net charge. Of the chelates tested, only the carcinogenic ferric nitriloacetate (Fe3+-NTA) was an effective catalyst of oxidation of all membranes, whether carrying a net charge, or not. We postulate that the unique catalytic capacity of the ferric nitriloacetate (Fe3+-NTA) can be explained by its existence in two forms at neutral pH, each binding to oppositely charged membranes and initiating their peroxidation. This gives the complex the unique ability to bind to any membrane, which may be a factor in its carcinogenicity.

Copper-catalyzed oxidative coupling of diphenylmethanimine promoted by some monodentate pyridines

Abstract Aerial oxidation of diphenylmethanimine to benzophenone azine was carried out at room temperature in the presence of cuprous chloride and a monodentate pyridine, such as pyridine, γ-picoline, isoquinoline, or quinoline. Cupric chloride, hydroxide, and nitrate were inactive in the reaction, whereas CuCl2KOH and Cu(OH)2HCl were active in the presence of excess pyridine. Bidentate ligands, such as ethylenediamine and monoethanolamine, inhibited the oxidative coupling of imine. Using isoquinoline for the direct synthesis of azine from benzophenone, ammonia and molecular oxygen, the reaction temperature can be lowered and the catalysts recycled.

Residue analysis of oleic acid in cucumber fruits after spraying sodium oleate solution

アブラムシ防除剤として開発中のオレイン酸ナトリウム液剤 (主成分としてシス-9-オクタデセン酸ナトリウムを20%含む) のキュウリ散布後のシス-9-オクタデセン酸 (ODA) の残留量を調べるため, ガスクロマトグラフによる分析を行なった. 二品種のキュウリ中の内因性ODA量は, それぞれ0.33, 0.37ppmであった. 二品種のキュウリ中のODA残留量は, 最終散布後1日目はそれぞれ1.81, 3.57ppmであり, 3日目においては, それぞれ0.88, 1.01ppmへと減少した. 未処理のキュウリにODAを2.5ppm添加し回収率試験を行なったところ, 回収率は102.0～108.4%の範囲にあった.

Study on the serum enzyme activity and nutrient intakes. Application of canonical correlation analysis.

The purpose of this study is to make clear a comprehensive relationship between two groups of serum enzyme activity and nutrient intakes. The first group of measurement include serum GOT, GPT, ALP activity and the second group of measurements include protein, fat, carbohydorate, vitamin A, B1, B2, C, animal protein, animal fat, age, and weight.These figures are taken from the epidemiological survey of Tokushima prefecture, including 252 males and 383 females, investigated from 1975 to 1980.Appling the canonical correlation analysis, we found the following results.1) In male, only two canonical correlations were statistically significant. The serum GOT and GPT activity related to body weight, further serum GOT activity was effected quality of food intakes.2) In Female, three canonical correlations were statistically significant. Both serum GOT and GPT activity related to protein intakes. High correlations were observed between serum GOT activty and vitamin B1, between serum GPT activity and vitamin C, between serum ALP activity and age.