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Publication
Featured researches published by Masao Sugiyama.
Insect Biochemistry and Molecular Biology | 1994
Yusuke Kato; Yoshiko Motoi; Kiyoko Taniai; Keiko Kadono-Okuda; Masanori Yamamoto; Yoshiaki Higashino; Michio Shimabukuro; Subrata Chowdhury; Jinhua Xu; Masao Sugiyama; Miyako Hiramatsu; Minoru Yamakawa
The formation of the lipophorin-lipopolysaccharide (LPS) complex in Bombyx mori hemolymph and its role in LPS detoxification were explored. LPS, an antibacterial protein inducer in insects, was injected into B. mori larvae. Analytical density gradient ultracentrifugation revealed that after injection the LPS peak shifts to a zone of lower density with time. The shifted peak was identified as the lipophorin-LPS complex. This complex formation was also achieved in an in vitro mixture of cell-free hemolymph and LPS at 25 degrees C but not at 1 degree C. The lipophorin-LPS complex had a significantly lower capacity to elicit the mRNA of cecropin B, an antibacterial protein. The biological activity of reextracted LPS from the complex was slightly reduced in the Limulus test and no structural modification was observed in sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE). These results suggested that the formation of lipophorin-LPS strikingly reduces the cecropin inducibility of LPS without any structural change in LPS. Similar serum lipoprotein-LPS complex formation and reduction of biological activities of LPS were also observed in mammals. We, therefore, suggest that the formation of the serum lipoprotein-LPS complex is a common pathway to inactivate LPS both in insects and in mammals.
Insect Biochemistry and Molecular Biology | 1995
Masao Sugiyama; Hisato Kuniyoshi; Eiji Kotani; Kiyoko Taniai; Keiko Kadono-Okuda; Yusuke Kato; Masanori Yamamoto; Michio Shimabukuro; Subrata Chowdhury; Jinhua Xu; Su Kyung Choi; Hiroshi Kataoka; Akinori Suzuki; Minoru Yamakawa
A Bombyx mori cDNA was cloned that hybridized with Hyalophora cecropia attacin probe and its nucleotide sequence was determined. This cDNA consisted of 846 nucleotides and the deduced amino acid sequence showed that the cDNA encodes an attacin precursor protein. The putative mature protein of B. mori attacin had 70.4, 68.3 and 18.8% identity in amino acid sequences with that of H. cecropia acidic and basic attacins and Sarcophaga peregrina sarcotoxin IIA, respectively. B. mori and H. cecropia attacins and S. peregrina sarcotoxin IIA had two subdomains in each G domain, suggesting that common amino acid residues in the subdomains are conserved during evolution and plays an important role in the activity of the antibacterial proteins. Expression of B. mori attacin gene was rapidly induced by the injection of Escherichia coli cells into B. mori larvae and continued at least for 48 h mainly in fat bodies and hemocytes.
Biochemical and Biophysical Research Communications | 1996
Akihiro Miyanoshita; Masao Sugiyama; Ai Asaoka; Kiyoko Taniai; Fumiko Yukuhiro; Minoru Yamakawa
Biochemical and Biophysical Research Communications | 1995
Subrata Chowdhury; Kiyoko Taniai; S. Hara; Keiko Kadono-Okuda; Yusuke Kato; Masanori Yamamoto; Jinhua Xu; Su Kyung Choi; Nitish C. Debnath; Hong Kyu Choi; Akihiro Miyanoshita; Masao Sugiyama; Ai Asaoka; Minoru Yamakawa
Biochemical and Biophysical Research Communications | 1995
Keiko Kadono-Okuda; Masanori Yamamoto; Y. Higashino; Kiyoko Taniai; Yusuke Kato; Subrata Chowdhury; Jinhua Xu; Su Kyung Choi; Masao Sugiyama; K. Nakashima; S. Maeda; Minoru Yamakawa
Biochemical and Biophysical Research Communications | 1996
Kiyoko Taniai; Takaaki Ishii; Masao Sugiyama; Akihiro Miyanoshita; Minoru Yamakawa
Applied Entomology and Zoology | 1996
Michio Shimabukuro; Jinhua Xu; Masao Sugiyama; Kiyoko Taniai; Keiko Kadono-Okuda; Yusuke Kato; Masanori Yamamoto; Subrata Chowdhury; Kyung Su Choi; Kyu Hong Choi; Akihiro Miyanoshita; C. Nitish Debnath; Minoru Yamakawa
The journal of sericultural science of Japan | 1995
Hong Kyu Choi; Kiyoko Taniai; Yusuke Kato; Keiko Kadono-Okuda; Masanori Yamamoto; Subrata Chowdhury; Jinhua Xu; Masao Sugiyama; Su Kyung Choi; Akihiro Miyanoshita; Nitish C. Debnath; Ai Asaoka; Takaaki Ishii; Minoru Yamakawa
Applied Entomology and Zoology | 2002
Toru Arakawa; Masao Sugiyama
The journal of sericultural science of Japan | 1996
Hong Kyu Choi; Su Kyung Choi; Kiyoko Taniai; Keiko Kadono-Okuda; Yusuke Kato; Masanori Yamamoto; Subrata Chowdhury; Jinhua Xu; Masao Sugiyama; Akihiro Miyanoshita; Nitish C. Debnath; Ai Asaoka; Minoru Yamakawa