Massimo Monteleone

The Red Seaweed Gracilaria gracilis as a Multi Products Source

In recent years seaweeds have increasingly attracted interest in the search for new drugs and have been shown to be a primary source of bioactive natural compounds and biomaterials. In the present investigation, the biochemical composition of the red seaweed Gracilaria gracilis, collected seasonally in the Lesina Lagoon (Southern Adriatic Sea, Lesina, Italy), was assayed by means of advanced analytical techniques, such as gas-chromatography coupled with mass spectrometry and spectrophotometric tests. In particular, analysis of lipids, fatty acids, sterols, proteins, phycobiliproteins and carbohydrates as well as phenolic content, antioxidant and radical scavenging activity were performed. In winter extracts of G. gracilis, a high content of R-phycoerythrin together with other valuable products such as arachidonic acid (PUFA ω-6), proteins and carbohydrates was observed. High antioxidant and radical scavenging activities were also detected in summer extracts of the seaweed together with a high content of total phenols. In conclusion, this study points out the possibility of using Gracilaria gracilis as a multi products source for biotechnological, nutraceutical and pharmaceutical applications even although more investigations are required for separating, purifying and characterizing these bioactive compounds.

Efficient and simple reactive milling preparation of photocatalytically active porous ZnO nanostructures using biomass derived polysaccharides

ZnO nanocrystals of different shapes and sizes have been synthesized using an innovative, simple and efficient dry reactive milling methodology using Zn(NO3)2 and various polysaccharides as sacrificial templates. Optimum results were achieved using extracted agar from the red seaweed Gracilaria gracilis. Upon template removal after calcination at 600 °C, the protocol gave rise to a range of porous metal oxide nanomaterials of different shapes and nanoparticle sizes which were found to have excellent photocatalytic properties in aqueous phenol degradation as compared to commercial P25 Evonik titania.

Cascade approach of red macroalgae Gracilaria gracilis sustainable valorization by extraction of phycobiliproteins and pyrolysis of residue

Phycobiliproteins extraction (primary refining) from Gracilaria gracilis seaweed, harvested in Lesina Lagoon (Italy) and further valorization of the residual algal via pyrolysis (secondary refining), were investigated with a cascade biorefinery approach. R-phycoerythrin (7 mg/g d.w.), allophycocyanin (3.5 mg/g d.w.) and phycocyanin (2 mg/g d.w.) were the main phycobiliproteins extracted. Pyrolysis of G.gracilis residue followed, aiming to investigate the production of bio-oil and biochar within a pyrolysis temperature range of 400-600 °C. Results showed that the bio-oil yield is high (∼65 wt%) at pyrolysis temperature ∼500 °C, but its high content in nitrogenous compounds prevents its use as a biofuel, unless some further de-nitrogenation takes place. Biochar yield ranged between 33 wt% (400 °C) and 26.5 wt% (600 °C). Interestingly, inorganic nutrients including P, K, Ca, Fe and Mg were detected in biochar, suggesting its potential use as recovering system of natural mineral resources from the oceanic reservoir.

Biochar application to peat based growing media for nursery production of broccoli seedlings

Conservation agriculture (CA) can improve sustainable rice production. This work evaluated the effect of different CA practices on rice agronomic system. A four-year experiment (2013-2016) was carried out in Crescentino (VC), North-West Italy, comparing three tillage managements (sod dry seeding, sod wet seeding and ploughing), combined with three N fertilisation levels (0-110160 kg N ha) and two straw management (straw retained and removed). Yield and yield components were influenced by both tillage practices and N fertilisation, but not by straw management. Sod seeding reduced grain yield of about 16% with respect to ploughing, because of the lower panicle density and higher sterility. Rice responded to N fertilisation, but the higher sterility limited the positive effect at high N rates. Moreover, in sod seeding, high N supply increased Apparent Recovery (AR). Straw retention improved soil quality, increasing soil organic carbon concentration.