Mauricio Osvaldo Moura

A preliminary analysis of insects of medico-legal importance in Curitiba, State of Parana

A survey of the carrion fauna was made at two sites in Curitiba, State of Paraná, with the objective of describing the insects associated with carrion and setting up a preliminary data-base for medico-legal purposes in south Brazil. Vertebrate exclusion experiments were carried out in each season between 1994 and 1995 with a 250 g laboratory-bred rat (Rattus norvegicus). Five stages of decomposition were identified: fresh, bloated, decaying, dry and adipocere-like. Some species showed seasonal and site preference and so could be used to identify the probable place and season where death took place. Sarconesia chlorogaster (Diptera, Calliphoridae) was restricted to an open field site and to cooler months. Hemilucilia semidiaphana (Diptera, Calliphoridae) and Pattonella resona (Diptera, Sarcophagidae) were restricted to the forest site and warmer months. Phaenicia eximia (Diptera, Calliphoridae) and Oxyletrum discicolle (Coleoptera, Silphidae) were present at both sites throughout the year and could be useful for population level analysis. Dissochaetus murray (Coleoptera, Cholevidae) was present throughout the year at the forest site and was associated with the adipocere-like stage. Ants played an important role producing post-mortem injuries to the carcasses. Insects of 32 species are reported as being useful in community level approaches.

Levantamento da fauna de Coleoptera que habita a carcaça de Sus scrofa L., em Curitiba, Paraná

This paper sought to assess the Coleoptera fauna associated with carcasses of Sus scrofa L., 1758, which is usually used as model in Forensic Entomology. The addition and sequential substitution of insect species could be used to estimate the post mortem interval (PMI). The present study took place in Centro Politecnico (UFPR), between september 2005 to september 2006. A pig weighting 15 kg was sacrificed each season and put inside a cage. Sampling is made daily in a tray placed below the carcass and in a Shannon modified trap, and each 14 days in five pit-fall traps. 4,360 beetles were collected, belonging to 112 species of 26 families, 12 were considered of forensic potential. The active collecting made in the tray was responsible for the largest number of beetles (2,023 specimens), followed by the modified Shannon trap (2,016 specimens) and by the pit-fall traps (324 specimens). Staphylinidae was more abundant in the modified Shannon trap, while Silphidae was more abundant in pit-fall traps. The main habits found are predator/parasite (55%) and omnivorous (38,05%), with only a few species considered necrophagous (1,31%).

Allozyme relationships in hypostomines (Teleostei: Loricariidae) from the Itaipu Reservoir, Upper Rio Paraná basin, Brazil

In an allozyme electrophoresis survey of 15 hypostomine species from the Itaipu Hydroelectric Reservoir, 25 loci from 14 enzyme systems were scored. Allozyme data allowed recording diagnostic genetic markers for all species analyzed and for some species groups within Hypostomus, a taxon which is taxonomically still unresolved in the Upper Rio Paraná basin. The mean expected heterozygosity of the species was considerably variable and hypotheses to tentatively explain this variation are discussed. A cladogram based upon the allelic frequencies of the species analyzed was produced by the continuous maximum likelihood method: Rhinelepis aspera and M. parananus were separated from the species of Hypostominae by a long branch length. Pterygoplichthys anisitsi was the sister of all the representatives of the genus Hypostomus. Within Hypostomus, two main clades were produced: in the first, H. cochliodon was the sister of the species comprising the H. plecostomus group, and in the second, the tree showed the following relationships: (H. albopunctatus (H. regani + Hypostomus sp. 3) + (H. margaritifer (H. microstomus (Hypostomus sp. 1 (H. ternetzi + Hypostomus sp. 2))))). Hypostomus ternetzi and Hypostomus sp. 2 are referred to here as representatives of the H. ternetzi group.

Chave para adultos de dípteros (Muscidae, Fanniidae, Anthomyiidae) associados ao ambiente humano no Brasil

Key to adult flies of dipterous species (Muscidae, Fanniidae, Anthomyiidae) associated to human habitats in Brazil. An identification key for the main 33 species of Muscidae, Anthomyiidae and Fanniidae occurring in association to human habitats in Brazil is presented. Most of the characters used for identification of the species are illustrated. Based on literature records, a list of the 65 anthropic species known to Brazil is also included.

Heterotrophic succession in carrion arthropod assemblages

A Sucessao ecologica em comunidades efemeras tem sido tratada como um processo discreto por varios autores, mesmo em detrimento de algumas evidencias que apontam para um processo continuo. Esta controversia aparentemente deve-se ao uso forense dos dados de sucessao. Baseado nestes fatos, nos analisamos dados da sucessao de insetos sobre carcacas em dois locais contrastantes fisionomicamente para determinar a natureza do processo de sucessao: se discreto ou continuo. Os padroes de visitacao demostraram uma complementaridade no padrao de chegada entre especies saprofagas e predadoras e que a taxa de recorrentes variou entre 40% e 46% com excecao do inverno com valores proximos a 95%. A area florestada sempre apresentou maior riqueza que a area urbana e, em ambas as areas, a tendencia ao aumento da riqueza seguiu um padrao exponencial com um declinio posterior. O padrao de chegada indica uma agregacao de especies nos primeiros estagios de decomposicao. Em ambas as areas nao foram encontradas separacoes claras entre as fases que compoem o processo de decomposicao, sugerindo assim, um processo continuo e sugerindo tambem, que a facilitacao nao e o processo que estaria gerando o padrao observado.

Variação espacial como mecanismo promotor da coexistência em comunidades de insetos necrófagos

A coexistencia em comunidades locais pode ser mediada atraves de varios mecanismos tais como competicao interespecifica e heterogeneidade espacial e temporal. Neste trabalho e analisada uma guilda de insetos necrofagos para se determinar o papel desempenhado pela agregacao na estrutura da comunidade. Um experimento foi delineado para se testar as premissas derivadas do modelo de coexistencia por agregacao que, em resumo, requer que no balanco entre a competicao intra e interespecifica, a primeira deva ser maior, para fazer com a coexistencia seja possivel. Os dados coletados compreendem dois protocolos: a coleta de adultos visitando a carcaca e a criacao das larvas coletadas na carcaca. Os resultados demonstram que 21 especies necrofagas foram criadas e 18 especies coletadas. Todas as especies mostraram-se distribuidas de forma agregada sobre os recursos e, embora varios valores negativos foram obtidos nos indice de associacao, a tendencia geral foi de uma covariacao neutra entre as especies. A analise confirma que a agregacao pode manter a diversidade nesta comunidade.

Host characteristics do not affect community structure of ectoparasites on the fishing bat Noctilio leporinus (L., 1758) (Mammalia: Chiroptera)

Patterns of parasite abundance and prevalence are thought to be influenced by several host characteristics such as size, sex, developmental stage, and seasonality. We examined two obligatory ectoparasites of the bat Noctilio leporinus (L.) (Chiroptera, Noctilionidae) to test whether prevalence and abundance of Noctiliostrebla aitkeni Wenzel and Paradyschiria fusca Speiser (Diptera, Streblidae) are influenced by the host characteristics. During this survey, 2110 flies were collected. The total abundance was 1150 N. aitkeni and 950 P. fusca. The prevalence of both species was shown to be superior to 75% and neither host size, sex, reproductive stage nor season influenced significantly the variation of the observed values. N. aitkeni were more abundant than P. fusca in all seasons except winter. Both flies showed a significant seasonal variation in terms of abundance but host biological characteristics (host size, sex, and reproductive stage) did not play a significant role as structuring factors of the batflies component community.

Diversity of the Astyanax scabripinnis species complex (Teleostei: Characidae) in the Atlantic Forest, Brazil: species limits and evolutionary inferences

The Astyanax scabripinnis species complex with its wide geographical distribution is an excellent model for evolutionary studies. Populations are usually geographically isolated but also, in some cases, occur in sympatry. In this study, five allopatric and/or sympatric populations of A. scabripinnis were analysed using geometric morphometry, cytogenetic markers, assays for induced breeding and phylogenetic inferences to draw conclusions on species limits and speciation processes in a natural setting. The morphometry of individuals indicated that the populations were well differentiated from each other. Cytogenetic evidence revealed a more conserved karyotypic macrostructure; however, molecular cytogenetic results obtained by in situ hybridization indicated 5S and 18S rDNA gene probe locations specific to each population. The reproduction tests for three locations suggest isolation between populations and the phylogenetic analyses suggest that the fish evaluated cluster in a monophyletic group. The combined data indicate that individuals are adapted to different environments in a complex evolutionary scenario, with linkage of populations during a recent geological period. However, due to reproductive isolation, the populations are evolving independently, reinforcing the existence of distinct cryptic species.

Diet and trophic niche of Lithobates catesbeianus (Amphibia: Anura)

Lithobates catesbeianus (Shaw, 1802) is an invasive anuran introduced in Brazil that is associated with the displacement and the decline of populations of native species worldwide. There is evidence that biological invasions are facilitated by certain attributes of the invading species, for instance niche breath, and that invasive species have a broader ecological niche with respect to native ones. We designed a study to ascertain the temporal, ontogenetic, and sex differences in the niche dynamics of the American bullfrog. We sampled monthly from June 2008 to May 2009 in the state of Parana, southern Brazil. For each individual, we gathered biometric and stomach content data. We then estimated the niche breath of the juveniles and adults, and compared it between the sexes. A total of 104 females and 77 males were sampled. Lithobates catesbeianus has a generalist diet, preying upon invertebrates and vertebrates. Even though the diet of the studied population varied seasonally, it did not differ between the sexes nor did it respond to biometric variables. Niche breadth was more restricted in the winter than in the autumn. The trophic niche of juveniles and adults did not overlap much when compared with the trophic niche overlap between males and females. Adult males and females had a considerable niche overlap, but females had a broader trophic niche than males in the winter and in the spring. These niche characteristics point to an opportunistic predation strategy that may have facilitated the process of invasion and establishment of this species in the study area.

Coleoptera Associated with Buried Carrion: Potential Forensic Importance and Seasonal Composition

ABSTRACT Burial has a unique effect on carrion fauna and this may affect the decomposition rate, consequently interfering with postmortem estimates. We surveyed the Coleoptera on buried rabbit carcasses between April 2008 and March 2009. The insects of forensic importance totaled 1,502 individuals, constituting 87.12% of the Coleoptera collected. The composition of the beetle community varied seasonally. Exposed carcasses lost weight much faster than buried ones. The weight lost by exposed cadavers in a week was greater than those carcasses buried for 10 wk. The temperature variations of the buried carcasses and the soil were around three times less variable than the environmental temperature. The proximity between the temperature variations of the soil and buried carcasses was because of the absence of larval masses. The results show differences in decomposition and faunal composition, demonstrating their relevance in the interpretation of cases with bodies in a similar situation.