Md. Abdul Alim

Refrigeration of Riptortus clavatus (Hemiptera: Alydidae) eggs for the parasitization by Gryon japonicum (Hymenoptera: Scelionidae)

Abstract Gryon japonicum (Ashmead) (Hymenoptera: Scelionidae) is a solitary egg parasitoid of Riptortus clavatus (Thunberg) (Hemiptera: Alydidae) one of the most important pests in soybean in Korean and Japan. Refrigeration of R. clavatus eggs for up to 120 days was evaluated as a method of mass production of G. japonicum. The refrigeration reduced the eclosion of eggs, thus no nymph emerged after 30 days of egg refrigeration. Increased duration of host acceptance behaviors by G. japonicum and some detrimental effects on parasitism rate, developmental time, longevity, and adult size of the parasitoid were found in refrigerated host eggs. However, all the biological parameters of G. japonicum were unaffected by the refrigeration of up to 30 days. Gryon japonicum parasitized 16 and 14 host eggs daily that were refrigerated for 15 and 30 days, respectively, which did not differ from parasitization of fresh host eggs. Furthermore, refrigeration of host eggs did not reduce the reproduction of the emerged adult parasitoids and emergence and sex ratio of their progeny. Gryon japonicum also parasitized 14 and 13 refrigerated host eggs per day kept at 26.3°C and 78.7% RH for 2 and 4 days of post-refrigeration without significant reduction, respectively. These results show that refrigeration of R. clavatus eggs can be a good method for mass rearing of the parasitoid, and the host eggs killed by cold storage can be supplemented in the field to boost field parasitism.

Refrigerated Eggs of Riptortus pedestris (Hemiptera: Alydidae) Added to Aggregation Pheromone Traps Increase Field Parasitism in Soybean

ABSTRACT Riptortus pedestris (F.) (Hemiptera: Alydidae) is a key pest of soybean [Glycine max (L.) Merr.] in Korea and Japan. Aggregation pheromone traps have been used to attract R. pedestris. An aggregation pheromone, produced by males of R. pedestris, also attracts the egg parasitoid, Ooencyrtus nezarae Ishii (Hymenoptera: Encyrtidae). As a new method of pest management, we propose the use of nonviable host eggs in the pheromone trap together to both trap R. pedestris and propagate parasitoids on host eggs attached to the trap. ≈Ten-thousand nonviable refrigerated host eggs were placed outside pheromone traps in three soybean fields (0.167–0.284 ha.). During the sampling period from 7 August to 8 October in 2008, statistical significance of treatment was not found in any life stage of R. pedestris population. However, significantly higher parasitism of both natural (78–91%) and refrigerated eggs (36–59%) occurred in the treatment sites, compared with control sites where parasitism was 0–62% and 16-34% on natural and refrigerated eggs, respectively. The proportion of bean pods with stink bug feeding damage was significantly reduced by 47% in the treatment sites. This study is the first trial of an integrated pest management (IPM) strategy using both nonviable host eggs and trap that simultaneously targets both the pest and its natural enemy.

Interspecific larval competition between two egg parasitoids in refrigerated host eggs of Riptortus pedestris (Hemiptera: Alydidae)

In a previous study, we found that soybean fields could be supplemented with refrigerated eggs of Riptortus pedestris (Fabricius) (Hemiptera: Alydidae) to enhance parasitism. As a part of a study to evaluate the effect of host egg refrigeration on parasitism, host acceptance behavior and interspecific larval competition between Gryon japonicum (Ashmead) (Hymenoptera: Scelionidae) and Ooencyrtus nezarae Ishii (Hymenoptera: Encyrtidae) were studied in multiparasitized unrefrigerated and refrigerated eggs. O. nezarae showed complete host acceptance behavior when offered refrigerated host eggs that were preparasitized by G. japonicum. Adult emergence rate of O. nezarae was 43 and 74% when the interval between the first and second oviposition was 0 and 4 days, respectively, and was not different between refrigerated and unrefrigerated eggs. Refrigeration did not change host acceptance behavior of G. japonicum, but adult emergence declined from 80% in unrefrigerated eggs to 37% in refrigerated eggs that were pre-parasitized by O. nezarae on the same day. No negative effects of refrigeration on sex ratio, adult longevity, and adult size of the both parasitoids were found. Generally host egg refrigeration did not negatively affect host acceptance behavior of the both parasitoids on preparasitized eggs or larval competition between the two parasitoids in multiparasitized host eggs with exceptions in the development time and emergence rate of G. japonicum. Therefore, host egg refrigeration may not interrupt interactions between the parasitoid populations in the field.

Ecotoxicological effect of insecticides on Ooencyrtus nezarae (Hymenoptera: Encyrtidae) reared from refrigerated and unrefrigerated Riptortus pedestris (Hemiptera: Alydidae) host

Due to increased field occurrence of Riptortus pedestris (Fabricius) (Hemiptera: Alydidae) on various crops including soybean, persimmon and apple in recent years in Korea, demand for insecticide applications to control the stink bug has increased. Acute toxicity of eight pesticides on Ooencyrtus nezarae Ishii (Hymenoptera: Encyrtidae), a major egg parasitoid of R. pedestris, was compared in the laboratory. Fenitrothion, spinosad, cyfluthrin, etofenprox and carbosulfan caused 100% mortality of O. nezarae within 24 hours by topical application or exposure to residue. Fenitrothion was also highly toxic to the parasitoid when ingested orally. In a previous study, release of refrigerated inviable eggs of R. pedestris was found to increase field parasitism; therefore, we evaluated the sublethal effect of fenitrothion when O. nezarae parasitised refrigerated or unrefrigerated host eggs. Although parasitism rates on both kinds of eggs significantly decreased when O. nezarae were provided with host eggs sprayed with fenitrothion, no difference in parasitism rate, adult emergence, sex ratio, development time and longevity of O. nezarae was found between the refrigerated or unrefrigerated host eggs when the insecticide was treated either before or after oviposition. There was no significant sublethal effect when parasitised host eggs were treated with the insecticide. From these results, all the insecticides tested showed high, acute toxicity against O. nezarae with relatively lower sublethal effects. Refrigeration of host eggs did not affect the susceptibility of O. nezarae to insecticides.

Bioassay of Plant Extracts Against Aleurodicus dispersus (Hemiptera: Aleyrodidae)

Abstract The spiraling whitefly, Aleurodicus dispersus Russell (Hemiptera: Aleyrodidae), is a destructive invasive pest in many parts of the world. Topical spray and dry film contact assays were conducted to measure the toxicity of 8 plant extracts and their mixtures traditionally used as insecticides in South Asian countries such as Bangladesh, India, and Nepal. The highest mortality (100%) of adults was recorded for neem (Azadirachta indica A. Juss.; Meliaceae) (ethanol) extract (500 mg/L) at 6 h after topical spray. This was followed by 5-leaved chaste tree (Vitex negundo L.; Lamiaceae) (ethanol), sweet sop (Annona squamosa L.; Annonaceae) (acetone), water pepper (Polygonum hydropiper L.; Polygonaceae) (acetone), banyan (Ficus benghalensis L.; Moraceae) (ethanol), banyan (acetone), and crown flower (Calotropis gigantea [L.] W. T. Aiton; Apocynaceae) (ethanol) extracts at 500 mg/L at 12 h after the spray. For the dry film method, the highest mortality (100%) of adults was also recorded for neem (ethanol) extract (500 mg/L) at 18 h after the treatment. Bioassay results indicate that neem (ethanol) extract mixed with crown flower (acetone), oleander (Nerium indicum Mill.; Apocynaceae) (acetone), or sweet sop (ethanol) (in the ratio of 1:1, 1:2, and 1:3 for each plant extract) showed synergism. Neem (ethanol) extract also showed the highest mean repellency rate (93%). In conclusion, neem, 5-leaved chaste tree, sweet sop, water pepper, banyan, and crown flower extracts showed good potential to control A. dispersus, and the mixtures of these plant extracts showed synergistic activity against A. dispersus.

Monitoring thrips species with yellow sticky traps in astringent persimmon orchards in Korea

Thrips are one of the insect pests of persimmon (Diospyros kaki Thunb.) in the major production areas of astringent persimmon in Korea. We surveyed astringent persimmon orchards in the Damyang, Sangju and Cheongdo regions of Korea to determine thrips species composition and abundance. Orchards sprayed with either organic or conventional pesticides were sampled over the course of one flowering season, using yellow sticky traps to determine if this is a suitable method for monitoring thrips populations, and to determine thrips species composition and abundance. Eight thrips species were captured on yellow sticky traps in both the tree canopy and ground cover: Ponticulothrips diospyrosi Haga et Okajima, Scirtothrips dorsalis (Hood), Frankliniella occidentalis (Pergande), F. intonsa (Trybom), Thrips tabaci (Lindeman), T. hawaiiensis (Morgan), T. coloratus (Schmutz) and T. palmi (Karny). In all regions, F. occidentalis and F. intonsa dominated in both organic and conventional orchards. S. dorsalis, F. occidentalis, F. intonsa and T. hawaiiensis were found in persimmon flowers, with S. dorsalis the dominant thrips. Significantly more S. dorsalis were captured from flowers in the lower and middle canopy than in flowers from the upper canopy. Fruit damage was also significantly higher in fruit from the lower canopy than in fruit from the middle and upper canopy.